🚨It's finally out!🥂 | A few years after solving the structure of human cis-PT, we now tackle its dynamics in
@NatureComms
, revealing that a conserved interfacial loop serves as an allosteric hub, communicating with the active site to regulate catalysis. rdcu.be/eShjl
Proud of this collaboration - our third paper on cisPT! Loved this one the most: a beautiful mix of high-res, low-res, and computational approaches. Grateful for this long-standing, truly fruitful team effort. Congrats to everyone!
Day 1 at the Structural MS Workshop in Biocev!
Straight into the lab! Hands-on HDX-MS, cross-linking, and native top-down MS.
🧪 ⚡ ❤️
Thanks to @spidocs.bsky.social, #OPAJK #Photomachines, @brukercorporation.bsky.social @ciisbinstructcz.bsky.social for the support!
#Biocev @mbuavcr.bsky.social
🎉Congratulations to the SPIDoc's teams and colleagues for the recent publication
"X-ray spectroscopy meets native mass spectrometry: probing gas-phase protein complexes"
👉https://doi.org/10.1039/D5CP00604J
🔥Selected as 2025 Physical Chemistry Chemical Physics HOT article🔥 @rsc.org
Great collaboration (as always)! And very funny story how HDX and native MS complement each other and reveal details about ions, substrates and mutations. We enjoyed this ride!
Free structural MS hands-on workshop @ BIOCEV (CZ)
📅 Sept 15–19, 2025
For PhDs, postdocs & undergrads (CZ/SK)
HDX-MS, native MS, cross-linking, high-res MS, data processing
Apply by July 31 → bit.ly/3HNyzIg
@spidocs.bsky.social @mbuavcr.bsky.social @ciisbinstructcz.bsky.social
Whenever we get a bioRxiv alert, we'are always curious to see what it brings. This time, the timing couldn’t have been better - a very pleasant surprise! 😄
biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
This was literally cool work. ❄️ We locked ourselves in the cold room to slow down the HDX and catch even the fastest dynamics. Bonus: It turns out you can manually capture a 2-second exchange reproducibly (next time we set up the quench flow!).
Get ready to take the next step in your career journey! 🚀 Join us for the SPIDoc's Career Day, connect with professionals and discover diverse career pathways.
When: May 16, 2025.
Where: Biocev, CZ or online.
For details, see the flyer peterslab.org/downloads/SP...
Utilization of high-resolution mass spectrometry and data-independent analysis to track the monoclonal antibody spatial stability
Expert Rev Proteomics. 2025. Prochazkova V, Rosulek M, Karpisek M, Matousek V, Novak P, Kukacka Z. @peterslab1.bsky.social
doi.org/10.1080/1478...
#MassSpec #Charlesuni
And it is done. Congratulations Drs. Marek, Michal and Lukas!
What a lovely day to close three great PhD chapters from our lab. Fingers crossed, guys!
Recommendations for Automating Hydrogen/Deuterium Exchange Mass Spectrometry Measurements using Data-Independent Acquisition Methods www.biorxiv.org/content/10.1101/2025.01....
Great collaboration. This time with some very nice HDX-MS and native MS. Thank you guys!
ProAlanase is AnPEP. So any ToleraseG, GlutenRid, Brewers Clarex, Clarity Ferm will work as well. 😉
This is actually what we did. Dietary supplement or a solution for beer brewing (Clarity Ferm). 😀
pubs.acs.org/doi/10.1021/...
10th Informal Proteomic Meeting #neformal2024
Czech Proteomic section of CSBMB
Nov 28 - 29, 2024, Olomouc
has just started
czproteo.cz/nps_aktualni/
Long overlooked but significant!
This is the post-Cys specificity in post-proline cleaving enzymes like AnPEP or neprosin. Good work Zuza
and the team!🎉🔥
pubs.acs.org/doi/10.1021/...
(illustration IG:kuba_draws_science)