Please share: the FMI is hiring a tenure-track Group Leader in Multicellular Systems to explore the molecular and cellular mechanisms driving the formation, maintenance, or destabilization of tissues, organs and organisms. Apply at: www.fmi.ch/education-ca...

How to rebuild your network on @bsky.app and improve your experience here?

I made a short starter guide with resources.
www.neuronaldynamics.eu/resources/bl...

Also, a thread 🧵 below.

Many people have asked me what's the deal with "SIO" in our Addgene vectors (www.addgene.org/Ofer_Yizhar/) - we use these in most of our Cre-dependent AAVs - so here's a brief explanation of how it works.

Thanks for the shout out :)

Site‐specific integration of DNA into wild‐type and mutant lox sites placed in the plant genome The bacteriophage P1 Cre—lox site-specific recombination system has been used to integrate DNA specifically at lox sites previously placed in the tobacco genome. As integrated molecules flanked by wi....

Lutz's lab showed that directional lox sites work in vertebrate cells. Originally, the generation of the mutant lox sites was done by Albert et al. 1995: doi.org/10.1046/j.13... to allow for the integration of a single gene copy into the plant chromosome.

One difference is that the DIO approach leads to the permanent removal of DNA, making it irreversible. For SIO an equilibrium between forward and reverse recombinantion is created. However the reverse probability is low, creating a bias to the activated state. In practice expression levels are good.

Let me in please 🙏

stGtACR & eOPN3

Got one trangene expressing IPSC clone after weeks of trying, finally grew enough to split it - sucked it right into the liquid waste 🙈

Broken Twitter bird with x-ed out eyes. Butterfly emerging from the wreckage.

Do you want to be my "new sciencetwitter" friend? 🤗