This wouldn’t have been possible without the great cooperation with the
@linderlab.bsky.social and the MarlovitsLab

The result: over 100 new candidate proteins—many of which are known actin regulators, others previously unlinked to actin.
This SLiM offers a framework to study modular actin interactions and identify novel cytoskeletal players.
#Cytoskeleton #MotifDiscovery

We aligned Lifeact and F-tractin bound to F-actin and discovered a structurally conserved motif.
Using this as a seed, we built a pipeline combining PSSM scoring, disorder prediction, and secondary structure filters to scan the human proteome.
#Lifeact #Ftractin

Discovery of a new evolutionarily conserved short linear F-actin binding motif Regulation of the actin cytoskeleton by actin binding proteins (ABPs) is essential for cellular homeostasis, and the mode of actin binding determines the activity of ABPs. Here, we discovered a novel ...

Lifeact and F-tractin are widely used actin markers—but did you know they bind F-actin via the same short linear motif?
We define a conserved F-actin-binding SLiM and uncover >100 proteins predicted to bind actin via this mechanism. biorxiv.org/content/10.1...
#Actin #SLiM