That camera view is good when they're on the perch or the roof of the box. There's a second camera view below that shows the inside of the box. The female is there at the moment incubating hey eggs
If you want a second stream to maximise the baby dinosaur action in a few weeks time....
peregrine.sites.sheffield.ac.uk
Ah, another day another game of "is it a cold or is it hay fever?"
#microsky Tour de force from the @pmoyniha.bsky.social @lislowe.bsky.social & @proftracypalmer.bsky.social labs on the Type VII secretion in Mycobacteria 🎉👏👏👏
Mine was a minuscule contribution, flattered to feature.
www.biorxiv.org/content/10.6...
Stylized M logo with gradient blue and green colors next to bold text reading Microbiology Society on a white background.
Just one day to go until the JMM seminar with Dr Robert Fagan! Be sure to catch their discussion on ‘Clostridioides difficile - an evolving AMR threat’. Register here: https://microb.io/4l58vI6 #JMM
Oh that is sad news. RIP Jeff
Find the hot-off-the-press plasmids here!
www.addgene.org/browse/artic...
Huge thanks to everyone involved! Hannah Fisher, Laurence de Lussy Kubisa, @funnirudh.bsky.social, Eloise Walker, Joseph Kirk, @peteroatley.bsky.social, Roy Chaudhuri, Gill Douce and @michaeljormsby.bsky.social
All of these plasmids use a consistent set of restriction sites so a single cloning strategy can be used to evaluate multiple promoters or tags and all cassettes are compatible with the knock-in plasmids for complementation or stable single copy expression
These can be used for identification of strains in mixed populations - particularly useful for competitive index experiments. You can potentially use 12 times fewer mice in infection studies.
Figure 4 from https://doi.org/10.1099/mic.0.001665 - showing the use of genetic barcodes to distinguish strains in mixed populations during competitive growth in vitro or in the mouse gut
Finally we generated a set of knock-in plasmids, including 12 with genetic barcodes that allow the C. difficile genome to be tagged with a 9bp sequence that allows unambiguous identification in sequencing datasets
Figure 2 from https://doi.org/10.1099/mic.0.001665 - showingWestern blot detection of 3xHA and 6xHis tagged proteins, and purification of a StrepTagged protein
Figure 3 from https://doi.org/10.1099/mic.0.001665 - showing fluorescence microscopy of C. difficile and C. sporogenes expressing a range of red fluorescent fusions
To the cloned gene you can add a number of different tags for purification, Western blot detection and fluorescence microscopy - His, HA, StrepTag, mCherry, mScarlet, SNAP, CLIP
Figure 1 from https://doi.org/10.1099/mic.0.001665 - showing promoter activity from a panel of constitutive and inducible promoters in three Clostridia species
We have created a set of modular plasmids with a selection of 10 constitutive and 2 inducible promoters - all of which have been benchmarked in C. difficile, C. sporogenes and C saccharoperbutylacetonicum
New paper from us.
If you're interested in genetic manipulation of Clostridia, we have a few new tools for you! 36 new plasmids - all available from @addgene.bsky.social
www.microbiologyresearch.org/content/jour...
#microsky
Ah! Sorry to hear that mate. I think MRC started sending the rejections today too
MRC?
She's been lead restricted for 3 weeks after spay - that wasn't fun. A lot happier now that she's a forest dog again. I think she is starting to settle a bit. We definitely had a bad few weeks around the 9 month point!
Yellow Labrador Pip asleep with her head on my shoulder. Just visible is the grey wet robe she's wearing
Our puppy turned 1 today. I think I managed to tire her out on a muddy walk!
They've separately said:
Our transition to new ways of working also means we unfortunately expect to make a reduced number of awards to applications currently being assessed by our four research boards and developmental pathway funding scheme.
www.ukri.org/blog/mrc-fun...
So despite Chapman acknowledging poor comms, they're still trying to spin this. "slightly lower" just isn't accurate, not even close. Discussing only 20% of submitted grants and funding at most 20% of these is not a *slightly* lower funding rate
Sir Ian McKellen performing a monologue from Shakespeare’s Sir Thomas More on the Stephen Colbert show. Never have I heard this monologue performed with such a keen sense of prescience. Nor have I ever been in this exact historical moment.TY Sir Ian, for reaching us once again.
#Pinks #ProudBlue
Colleagues of mine @naomilpollock.bsky.social and @alice-rothnie.bsky.social are at a BBSRC event today and have been told that the RM call is paused, until maybe March, to allow them to transition to a open call rather than 3 deadlines a year. Nice that this is widely communicated 😉
A master class on how not to do comms.... Thanks for the info Alan!
That's much more positive than the news from MRC - first good news I've heard in the last week
Have you seen anything to suggest when assessments will restart? That's what's missing from the absolutely appalling communication from UKRI. Even a few months delay is going to leave a huge number of postdocs and finishing PhDs high and dry
Absolutely spot on Ali - I think your quotes are excellent
These are the life-blood of science. All that lab knowledge lost, careers ended. All the investment in their training to this point lost to academic research. That's the human cost of UKRI's "review period to develop implementation"
I know what they're getting at with the "could put jobs at risk" but can we focus on the immediate impact? Each suspended round at MRC and BBSRC means about 80 fewer postdoc jobs and the retrospective cuts at MRC add another 30 or so
Leaks everywhere and finally we get an official statement, one that somehow manages to say a lot while; No timelines. No clarity. No acknowledgement of impact on ECRs whose careers depend UKRI funding.
“Later this year” isn’t a plan. And the sector deserves better than reassurance without detail.
