Check out our new release on BioRxiv
co-lead by @pm-mueller.bsky.social

👉 rb.gy/lnrizk

special🙏 to
Severine Kunz #MDC
@leventallab.bsky.social
@andimicroscopy.bsky.social
@ewerslab.bsky.social and Kedar Narayan @NCI CCR VolumeEM

Are the days of discovering new cellular structures over?? /1

A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...

Our paper is now out in Science! Super excited to share our discovery that #mitochondria #pearling is the elusive mechanism driving the regular distribution and inheritance of #mtDNA nucleoids 🧬 [1/6]

Very cool. In many cell types it doesn’t quite circle. Just sort of hops from cluster of mito to neighboring cluster. Has a a lot to do with how spread out the cell is.

Ooh that looks familiar. What’s the cell type?

Not something you see in textbooks very often: tripolar mitosis.

Yup. This is EB3 fused to mNeonGreen

I’ll have a look at the raw data tonight but I think you’re in the right ballpark.

Thanks!

Of course - let me know if you need any additional files.

An intracellular meteor shower. EB3 comets tracking growing microtubule plus-ends in a cultured cell.

Dynamic actin waves in a zebrafish embryo. Credit to @aaandmoore.bsky.social & Dvir Gur. #ZebrafishZunday 🧪

🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!

Image of an animal cell showcases web-like fibers called vimentin — a protein that provides mechanical stability to cells and tissues

Created using fluorescence microscopy, this stunning image of an animal cell showcases thin, web-like fibers called vimentin — a protein that provides mechanical stability to cells and tissues, despite a single vimentin being ~7000x smaller than the width of a human hair. 📸 @aaandmoore.bsky.social

Direct labeling of microtubule turnover reveals in-lattice repair and stabilization patterns in developing neurons The microtubule cytoskeleton is the backbone of neuronal morphogenesis, driving the development of the dendrites and axon, and supporting trafficking to distant compartments. How neuronal microtubules...

🚨The Neurocyto lab is branching out in our latest preprint! We used tubulin microinjection to directly visualize microtubule turnover in developing hippocampal neurons, demonstrating the presence of in-lattice repair and a selective stabilization in the nascent axon. Check below, or read on 🧵 1/9

It typically localizes to the endoplasmic reticulum.

Call it what you want but it’s technically still just the Golgi Apparatus until you get an act of Congress.

I don't always get pretty, isolated, AND transfected primary neurons in culture, but when I do I take advantage.... Rat hippocampal neuron overexpressing ThymosinB4-mScarlet and imaged for 16hr on a @zeiss-microscopy.bsky.social LSM880 with Airyscan. #FluorescenceFriday #Microscopy

This movie shows lysosomes (orange) and keratin (gray) in a cultured cell over 10 minutes.

I like this movie, but a friend of mine likes to complain about the obvious stitching artifacts. I'll try harder next time, Michael. Vimentin (orange) and ER (blue) in an overnight acquisition.

What's up Bluesky....here's some live-cell imaging taken in my lab

is there a direct crosstalk between actin and vimentin intermediate filaments? Our work shows that vimentin promotes actin assembly by stabilizing ATP-subunits at the barbed end.
Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social

www.biorxiv.org/content/10.1...

it’s a little transparent, but that’s the scale bar in the bottom left corner. 5 micrometers.

Still posting cytoskeleton videos, it seems. Actin this time.

Sample: Lifeact-eGFP in HeLa cells.
Modality: Airyscan confocal

Timestamp is mm:ss and the scale bar is 5 µm.

24FPS_VimentinCos7GIF_4320p_ultraHQ This is "24FPS_VimentinCos7GIF_4320p_ultraHQ" by Andy Moore on Vimeo, the home for high quality videos and the people who love them.

vimeo.com/1139537563?s...

I uploaded a version of that vimentin IF movie to my vimeo page. It's still compressed, but looks a lot better. Best results when you set the quality to 4k.

Yup - imaged it yesterday on a laser scanning confocal.

Yup!

Fun fact - this protein belongs to the same family as the hard keratins found in all hair (including the hair you might find in a shower drain).

We should promote IFs and septins to position 1 and 2. Actin filaments and microtubules have had their day in the sun. We can just start calling them the third and fourth components 😂

Sure - these are vimentin intermediate filaments which are part of the cell’s internal scaffolding. They confer mechanical resilience to cells and , among other things, help to anchor and position organelles like mitochondria. These filaments are part of the same family that includes keratins.