Unveiling the Molecular Secrets: A Comprehensive Review of Raman Spectroscopy in Biological Research Raman spectroscopy has been proven to be a fast, convenient, and nondestructive technique for advancing our understanding of biological systems. The Raman effect originates from the inelastic scatteri...

pubs.acs.org/doi/10.1021/...

Development of a candidate reference sample for the characterization of tip-enhanced Raman spectroscopy spatial resolution Tip-Enhanced Raman Spectroscopy (TERS) is a topographic and chemical analysis technique with nanoscale resolution, consisting of the combination of Scanning Probe Microscopy (SPM) and Localized Surfac...

Important work towards reproducible spatial analysis using SERS.

doi.org/10.1039/C8RA...

Real‐Time Monitoring of In Vitro Transcription for Production of mRNA Using Raman Spectroscopy Schematic depicting the workflow for model building and validation using Raman spectroscopy for real-time monitoring of IVT.

analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...

Invitro-transcription studied using Raman spectroscopy. Very interesting !

#mRNA
#Raman

Present and Future of Surface-Enhanced Raman Scattering The discovery of the enhancement of Raman scattering by molecules adsorbed on nanostructured metal surfaces is a landmark in the history of spectroscopic and analytical techniques. Significant experim...

pubs.acs.org/doi/10.1021/...

Fundamental aspects !
Temperature Dependence of Coherent versus Spontaneous Raman Scattering

journals.aps.org/prl/abstract...

Fundamentals and Applications of Raman‐Based Techniques for the Design and Development of Active Biomedical Materials The potential and benefits of Raman spectroscopy as an analytical method for non-invasive biomaterial characterization are presented. After a comprehensive description of the theoretical and practica...

[Review] Raman for design and development of active biomedical materials.

advanced.onlinelibrary.wiley.com/doi/10.1002/...

Raman Techniques: Fundamentals and Frontiers - Discover Nano Driven by applications in chemical sensing, biological imaging and material characterisation, Raman spectroscopies are attracting growing interest from a variety of scientific disciplines. The Raman e...

Core Raman foundations: Jones et al. explain fundamental Raman physics, experimental setups for spontaneous and enhanced Raman, and their main strengths and limits
link.springer.com/article/10.1...

A Novel Two-Step Method for the Detection of Organic Gunshot Residue for Forensic Purposes: Fast Fluorescence Imaging Followed by Raman Microspectroscopic Identification Gunshot residue (GSR) is potentially key evidence during a criminal investigation of a shooting accident. Current standardized forensic science methods target the detection of inorganic GSR (IGSR). In...

Proof-of-concept two-step method combining fast fluorescence imaging with confirmatory Raman microspectroscopy to reliably detect and identify organic gunshot residue.
doi.org/10.1021/acs....

A review of Raman spectroscopy of vanadium oxides We review the available data on the structures and Raman spectra of vanadium oxides, VOx. We also present our own results concerning such phases as α- and β-vanadium, V14O6, VO, V2O3, V3O5, several p....

Noteworthy early works on metal oxides studied using Raman spectroscopy

Titanium dixode : doi.org/10.1002/jrs....
Vanadium oxide : doi.org/10.1002/

🚀 Excited to share my VAMAS project, within TWA 42 – Raman Spectroscopy and Microscopy! Focussing on Raman intensity calibration, strengthening materials metrology and comparability across laboratories worldwide 🌍⚙️ #Raman #RamanSpectroscopy #calibration [https://www.vamas.org/twa42/index.html]

Artificial Intelligence-Powered Raman Spectroscopy through Open Science and FAIR Principles Raman spectroscopy is a fast-growing and increasingly powerful analytical technique applied across diverse disciplines such as materials science, chemistry, biology and medicine. This growth is driven...

doi.org/10.1021/acsn...

Enhancement Factors: A Central Concept during 50 Years of Surface-Enhanced Raman Spectroscopy In this Perspective, we provide an overview of the core concepts around surface-enhanced Raman spectroscopy (SERS) enhancement factors (EFs), including both theoretical and experimental considerations...

Perspective article discussing enhancement factors relevant to SERS. doi.org/10.1021/acsn...

Posthandling Spectral Information Enhancement for Single Cell Raman Molecular Mapping Analysis Biochemical analysis of living systems such as single cells benefits greatly from the label-free and low-invasive molecular mapping with Raman microspectroscopy. Sets of Raman spectra at different spatial points are analyzed to generate Raman molecular maps corresponding to specific chemical species. However, human error and subjective data analysis can be technical issues that limit interpretation and its validity. Here, we present an objective data analysis scheme for postprocessing large data sets of Raman spectra for molecular mapping of living cells. The process comprises three steps: (i) Denoising the spectral data set using low-rank approximation; (ii) obtaining an objective background from data points outside the target cell; (iii) subtracting the thus obtained background using Hypothetical Addition Multivariate Analysis with Numerical Differentiation (HAMAND) via an automatically determined coefficient. Through the present analysis, minor Raman peaks, as indiscernible as they are, can be identified and precisely mapped. We demonstrate a quantitative discussion of cellular components after extracting contributions only from a single target cell from a Raman mapping image where multiple cells or parts of other cells are present. This work opens an improved analysis workflow for accurate spectroscopic analysis of living cells with the advantage of identifying minor Raman peaks unambiguously.

By refining how we process Raman information, the study transforms every cell from a blur of chemistry into a clearly textured molecular landscape.

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Isotope labeling for cellular dynamics !

www.sciencedirect.com/science/arti...

Direct imaging of intracellular RNA, DNA, and liquid–liquid phase separated membraneless organelles with Raman microspectroscopy - Communications Biology Raman spectroscopy is employed to show simultaneous, label-free imaging of RNA, DNA, and proteins, revealing secondary structural features of intracellular LLPS organelles, and condensed chromosome.

Liquid liquid phase separation resolved in cells
doi.org/10.1038/s420...

Exploring feature extraction methods for Raman spectroscopy: A comparative study Raman spectroscopy is a robust, non-destructive analytical technique that offers detailed insights into the chemical composition, molecular structure,…

www.sciencedirect.com/science/arti...

Measurement of Lateral Resolution in Confocal Raman Spectroscopy Using Indium Arsenide Nanowires Lateral resolution is a key performance parameter for confocal Raman spectroscopy. We propose a protocol using an indium arsenide nanowire reference sample to quantitatively measure the resolution. T....

analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/...

Quantification of titanium dioxide (TiO2) anatase and rutile polymorphs in binary mixtures by Raman spectroscopy: an interlaboratory comparison - IOPscienceSearch Quantification of titanium dioxide (TiO2) anatase and rutile polymorphs in binary mixtures by Raman spectroscopy: an interlaboratory comparison, Sacco, Alessio, Mandrile, Luisa, Tay, Li-Lin, Itoh, Nobuyasu, Raj, Ankit, Moure, Alberto, Del Campo, Adolfo, Fernandez, Jose F, Paton, Keith R, Wood, Sebastian, Kwon, Hyuksang, Adel, Tehseen, Hight Walker, Angela R, Martins Ferreira, Erlon Henrique, Theissmann, Ralf, Koch, Thomas, Giovannozzi, Andrea Mario, Portesi, Chiara, Rossi, Andrea Mario

Bringing Raman Metrology for the analysis of titanium dioxide, a useful material for industrial and catalysis.

iopscience.iop.org/article/10.1...

doi.org/10.1002/jrs.... "Application of Raman spectroscopy for the detection and identification of microorganisms"

Raman spectroscopy in cell biology and microbiology This review describes current progresses of Raman spectroscopy in characterizing and imaging living eukaryotic cells, bacteria, and viruses. The Raman spectrum, specific to different types of unicell...

New perspectives in cell biology (identification, classification and more) studied via Raman spectroscopy. Check out this review work :

doi.org/10.1002/jrs....

A Short Review of Cavity-Enhanced Raman Spectroscopy for Gas Analysis The market of gas sensors is mainly governed by electrochemical, semiconductor, and non-dispersive infrared absorption (NDIR)-based optical sensors. Despite offering a wide range of detectable gases, ...

doi.org/10.3390/s210...

From Genotype to Phenotype: Raman Spectroscopy and Machine Learning for Label-Free Single-Cell Analysis Raman spectroscopy has made significant progress in biosensing and clinical research. Here, we describe how surface-enhanced Raman spectroscopy (SERS) assisted with machine learning (ML) can expand its capabilities to enable interpretable insights into the transcriptome, proteome, and metabolome at the single-cell level. We first review how advances in nanophotonics–including plasmonics, metamaterials, and metasurfaces–enhance Raman scattering for rapid, strong label-free spectroscopy. We then discuss ML approaches for precise and interpretable spectral analysis, including neural networks, perturbation and gradient algorithms, and transfer learning. We provide illustrative examples of single-cell Raman phenotyping using nanophotonics and ML, including bacterial antibiotic susceptibility predictions, stem cell expression profiles, cancer diagnostics, and immunotherapy efficacy and toxicity predictions. Lastly, we discuss exciting prospects for the future of single-cell Raman spectroscopy, including Raman instrumentation, self-driving laboratories, Raman data banks, and machine learning for uncovering biological insights.

doi.org/10.1021/acsn...

RamanSPy: An Open-Source Python Package for Integrative Raman Spectroscopy Data Analysis Raman spectroscopy is a nondestructive and label-free chemical analysis technique, which plays a key role in the analysis and discovery cycle of various branches of science. Nonetheless, progress in Raman spectroscopic analysis is still impeded by the lack of software, methodological and data standardization, and the ensuing fragmentation and lack of reproducibility of analysis workflows thereof. To address these issues, we introduce RamanSPy, an open-source Python package for Raman spectroscopic research and analysis. RamanSPy provides a comprehensive library of tools for spectroscopic analysis that supports day-to-day tasks, integrative analyses, the development of methods and protocols, and the integration of advanced data analytics. RamanSPy is modular and open source, not tied to a particular technology or data format, and can be readily interfaced with the burgeoning ecosystem for data science, statistical analysis, and machine learning in Python. RamanSPy is hosted at https://github.com/barahona-research-group/RamanSPy, supplemented with extended online documentation, available at https://ramanspy.readthedocs.io, that includes tutorials, example applications, and details about the real-world research applications presented in this paper.

pubs.acs.org/doi/10.1021/...

Raman ConvMSANet: A High-Accuracy Neural Network for Raman Spectroscopy Blood and Semen Identification Animal blood and semen analysis plays a significant role in national biological resource management, wildlife conservation, and customs security quarantine. Traditional blood analysis methods have dis...

Today we bring AI and ML applications with Raman spectroscopy.

pubs.acs.org/doi/10.1021/...

Graphene edge method for three‐dimensional probing of Raman microscopes focal volumes A novel method for the accurate measurement of the dimensions of the focal volumes of Raman microscopes, using the edge of a graphene flake, was devised. In this work, it is explained in detail and a...

Translating graphene to determine the confocal volume in a confocal Raman setup !

doi.org/10.1002/jrs....

When cells divide: Label-free multimodal spectral imaging for exploratory molecular investigation of living cells during cytokinesis - Scientific Reports Scientific Reports - When cells divide: Label-free multimodal spectral imaging for exploratory molecular investigation of living cells during cytokinesis

Living cells studied with focus on cell cycle utilizing Raman spectroscopy. A case of S. pombe

doi.org/10.1038/srep...

Probing the Nature of Defects in Graphene by Raman Spectroscopy Raman spectroscopy is able to probe disorder in graphene through defect-activated peaks. It is of great interest to link these features to the nature of disorder. Here we present a detailed analysis o...

Adding few more papers here : doi.org/10.1021/nl30...

Probing the Nature of Defects in Graphene by Raman Spectroscopy Raman spectroscopy is able to probe disorder in graphene through defect-activated peaks. It is of great interest to link these features to the nature of disorder. Here we present a detailed analysis o...

doi.org/10.1021/nl30...

Probing the Nature of Defects in Graphene by Raman Spectroscopy Raman spectroscopy is able to probe disorder in graphene through defect-activated peaks. It is of great interest to link these features to the nature of disorder. Here we present a detailed analysis o...

doi.org/10.1021/nl30...

Raman spectroscopy as a versatile tool for studying the properties of graphene - Nature Nanotechnology Advances in the understanding of Raman processes in graphene have made it an essential tool for studying the properties of this one-atom-thick carbon material.

Graphene which has many applications is well characterized using Raman spectroscopy. Check out these papers.

doi.org/10.1038/nnan...