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Posts by Dr Benjamin Sharpe

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📅 12 Oct | 10:00–17:00
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#Bioimaging

3 weeks ago 19 8 1 1

I often use methyl green and excite with 633 laser instead if on fixed cells, if I don't have any other far red dye on that channel. I really hate how broad DAPI's emission spectrum is!

1 month ago 5 0 0 0
Introduction to CellProfiler - by a biologist, for biologists!
Introduction to CellProfiler - by a biologist, for biologists! YouTube video by CellMorphoJSero

If you or your trainees are just getting started with image analysis of cells & are #CellProfiler -curious, here is a fairly thorough walk-through demo I did recently. Loading files, extracting metadata (fun with Regex!), identifying objects, measuring, filtering, QC, exporting:
youtu.be/fO5nJdhj_7I

4 months ago 82 33 4 0

Even if HPV only caused cancer in people with cervixes, it would be essential to vaccinate people without cervixes because they can carry and spread the virus. You don't control infectious diseases by only vaccinating the people who might die, you control them by vaccinating everyone.

4 months ago 572 113 11 4
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SWBio DTP PhD Project: Mapping the epigenetic landscape in cells under compressive forces at University of Bath on FindAPhD.com PhD Project - SWBio DTP PhD Project: Mapping the epigenetic landscape in cells under compressive forces at University of Bath, listed on FindAPhD.com

We have a multidisciplinary PhD position on offer, through a BBSRC funded doctoral training program, open to UK and International students at Bath Uni. Microfluidics, microscopy, mechanobiology and epigenetics!
www.findaphd.com/phds/project...

5 months ago 5 4 0 0

Always shocking to see these kinds of data collected together. "[publishers made]US$12 billion in profits between 2019 and 2024" - That's $12 billion diverted away from actual research or materially supporting the community - these are disgusting numbers.

5 months ago 22 13 0 0
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James Watson in his own words “Some anti-Semitism is justified” “Whenever you interview fat people, you feel bad, because you know you’re not going to hire them” “Japan should be bombed for d…

If you're a scientist our journalist writing a piece about Watson and his "complex and nuanced character", you're deliberately ignoring the fact that his views made him one of the most extreme racist and sexist scientific "leaders" of his time.
liorpachter.wordpress.com/2018/05/18/j...

5 months ago 17 5 0 0

I've heard it called a "pedigree" before at conferences and the whole idea of it makes me sad to be honest. We're supposed to be smart people, you would think we would know better!

5 months ago 0 0 1 1
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Please re-post!

5 months ago 7 5 1 0
Screenshot of LinkedIn’s menu: “How LinkedIn uses your data”. Near the bottom, is the option to turn off “Data for Generative AI improvement”, shown with a red arrow.

Screenshot of LinkedIn’s menu: “How LinkedIn uses your data”. Near the bottom, is the option to turn off “Data for Generative AI improvement”, shown with a red arrow.

🤖 If you use LinkedIn, please be aware that they automatically use your profile to train their GenAI. To turn off, go to Settings > Data Privacy > Data for Gen AI improvements.

6 months ago 121 90 7 4

Besides the fact that "unfunded" research often yields the greatest breakthroughs and generates necessary preliminary results for "funded" research, this idea is analogous to the FA proposing to cease supporting football outside the Premier League. Chip away at a pyramid and the pinnacle also sinks.

7 months ago 6 4 2 1
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Application of the DNA-Specific Stain Methyl Green in the Fluorescent Labeling of Embryos Methyl green has long been known as a histological stain with a specific affinity for DNA, although its fluorescent properties have remained unexplored until recently. In this article, we illustrate t...

pmc.ncbi.nlm.nih.gov/articles/PMC...
The dye is a pain to prepare with serial extraction in chloroform but once you've done it, you'll have enough for several lifetimes!

7 months ago 2 0 0 0

Honestly I'm really loving methyl green. You don't have to use much, it's really bright and you can excite it with 633nm laser so it doesn't bleed through into 488 as badly as DAPI can

7 months ago 2 0 1 0

We’re launching @thebacr.bsky.social webinars! Register to attend for free via MS Teams using the QR code below.

1 year ago 2 2 0 0

Happy Christmas Tim 🥰

1 year ago 1 0 0 0