Delighted to see our last work published, showing that Wadjet-I SMC hinges allow the selective bypass of obstacles on DNA!
At the @gruberlab.bsky.social, with @drhonsworth.bsky.social and @taschner.bsky.social, and with James & @dnatopologylab.bsky.social

Happy that the final version of our Lamassu work @yli18smc.bsky.social is now out:

www.nature.com/articles/s41...

Thanks again to our awesome collaborators @mblokesch.bsky.social and David and co and Mark Szczelkun and @steven-shaw.bsky.social and the DCI Lausanne @fbm-unil.bsky.social

Very nice collaboration with the BSI platform at @igbmc.bsky.social. Streptavidin grids were instrumental to solve a rather drastic anisotropy problem!

Thank you Biswajit 😊

Many thanks to the reviewers for their thoughtful and constructive comments on our manuscript.

Delighted to announce our latest work on how Wadjet loads onto and establishes topological entrapment of plasmid DNA prior to initiating loop extrusion. At @gruberlab.bsky.social, Dubochet Center for Imaging and BSI @igbmc.bsky.social

www.cell.com/molecular-ce...

Our Wadjet-II paper by @roisnehamelinf.bsky.social is out in polished form: www.cell.com/structure/fu...

With a great preview by @kevincorbett.bsky.social & @amardeeep.bsky.social highlighting the potential of Wadjets:

Wadjet—Keeping a watchful eye on circular DNA.

www.cell.com/structure/fu...

Telomeres stall DNA loop extrusion by condensin Analikwu et al. show that dense linear arrays of the telomeric protein Rap1 on DNA block DNA loop extrusion by condensin. Rap1-induced stalling depends on array length and protein density and leads to a local chromatin decompaction during anaphase, revealing how protein arrays can regulate SMC-driven genome organization.

Telomeres stall DNA loop extrusion by condensin

This reveals parallels with DNA end protection in eukaryotes and highlights the challenges of having linear chromosomes as well as functional repair of DNA double strand break.

www.biorxiv.org/cgi/content/...

New tenure track assistant professor position in molecular microbiology in our department @dmf-unil.bsky.social @unil.bsky.social! We are casting a wide net for an experimental molecular microbiologist. Apply here: wwwfbm.unil.ch/releve/appli...

Fantastic teamwork with @drhonsworth.bsky.social, @taschner.bsky.social, in the @gruberlab.bsky.social with James at @dnatopologylab.bsky.social

Happy to share our latest work. Wadjet-I SMC hinges are gates allowing selective bypass of obstacles connected through ssDNA BUT are dispensable for extrusion of naked DNA.

www.biorxiv.org/content/10.1...

The SMC Hinge is a Selective Gate for Obstacle Bypass www.biorxiv.org/content/10.1101/2025.03....

Screenshot of 2d ligand environment view, 1x8b 4dfr, 2vtq, 2p4y

Coot 1.1.14 has been released!

Coming to homebrew and flatpak soon!

- Clark & Labute comes to Coot 1, Moorhen & chapi!
(svg, 2D ligand environment view)
- Solid Symmetry (symmetry that you can see!)
- PAE plot for AlphaFold models
#coot
#molmatinf

Alt : Structure of the apo Lamassu protein complex. Schematic model of the active LmuA tetramer nuclease (top right). Lamassu icon (top left).

Glad to share the work of @yli18smc.bsky.social and co on Lamassu, a bacterial defense system related to Rad50/Mre11 (RM). While RM carefully trims DNA ends for repair, Lamassu chops up the host chromosome. Our study reveals how it is regulated to minimize damage, activating only during infection.

Side view of the structure of a monomer of type II Wadjet.

Happy to share the beautiful structure of a Wadjet SMC complex by @roisnehamelinf.bsky.social, with support from the DCI and @drhonsworth.bsky.social ! This is a type II Wadjet with unique characteristics including a tandem KITE subunit, distinct coiled coil architecture and a deviant hinge.

Structure of a Type II SMC Wadjet Complex from Neobacillus vireti www.biorxiv.org/content/10.1101/2025.03....

Soutien aux projets en cryo-microscopie électronique: découvrez les 4 récipiendaires de la FBM Pour cette toute première mise au concours en cryo-microscopie électronique, la Commission de la Recherche de la FBM a retenu 4 projets innovants qui seront menés au sein des Départements de microbiol...

Soutien aux projets en cryo-microscopie électronique: découvrez les 4 récipiendaires @fbm-unil.bsky.social
➡️ www.unil.ch/news/1740986...

👏 Félicitations à Paula Navarro, Cyrielle Hou, Violeta Block, Florian Roisné-Hamelin 👏

@unil.bsky.social @dmf-unil.bsky.social @dib-unil.bsky.social

Pleased to announce the publication of our latest work on how Wadjet senses DNA circularity prior to cleavage. Collaborative effort with @drhonsworth.bsky.social at @gruberlab.bsky.social & Dubochet Center for Imaging. Deep thanks to our advisors and reviewers.

authors.elsevier.com/c/1iXM53vVUP...

Structures of JetABCD complex on kinked plasmid DNA.

First preprint 📢 on bsky: New work on the Wadjet defence system by @roisnehamelinf.bsky.social & @drhonsworth.bsky.social revealing beautiful & exciting structures of JET on plasmid DNA. With loop extrusion, obstacles, DNA bending, kinking, cleavage 🦠🧫 🧪
t.co/RaO7IO3zDk 
With help from @DCI_Lausanne