A series of spontaneously blinking dyes for super-resolution microscopy - Nature Methods A series of spontaneously blinking dyes in the far-red range facilitate single-molecule localization microscopy. These dyes vary in their blinking properties and can be matched to the applications and...

Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...

GitHub - saalfeldlab/lens-correct: Correcting lens-distortion in confocal stacks Correcting lens-distortion in confocal stacks. Contribute to saalfeldlab/lens-correct development by creating an account on GitHub.

If your imaging system has poorly characterized constant distortions leading to chromatic aberration, shoddy stitching, or false measurements (the answer is likely yes), you may want to give this tool a try. Native installers for Linux, Mac, Win under Releases github.com/saalfeldlab/...

So many great conferences @hhmijanelia.bsky.social . Don't miss the opportunity to participate in one of these unique experiences! www.janelia.org/you-janelia/...

Are you working at the interface of #neuroscience and ML/AI? Share your research at Janelia in Nov! 🧠 🤖 janelia.news/NAI26

We’re excited to announce the next Fluorescent Proteins & Biological Sensors conference! Abstract submissions are now open—hope to see you there.
www.janelia.org/you-janelia/...

🧪 We are excited to share this novel open access paper on Phasor Mixing Coefficient to analyze colocalization, developed by folks @i2janelia.bsky.social and @aicjanelia.bsky.social. This is also the first technical paper jointly published with our sister imaging center @malacridalab.bsky.social!

Wow, @erturklab.bsky.social team did it again! Bringing science fiction to the real world 👋👋

really cool, congrats! #microscopy

How do I get started with image analysis? - FocalPlane How do I get started with image analysis? - AIC at HHMI Janelia

Getting started with analysis can be daunting, especially given the many tools available. In our latest @focalplane.bsky.social post, @scientistrachel.bsky.social & Owen Puls discuss framing analysis questions to extract informative biological information. focalplane.biologists.com/2025/12/15/h...

Pretty cool tool! Would have been extremely useful during my academic career. #Microscopy

AIC at HHMI Janelia Introduction to the AIC at HHMI Janelia blog on FocalPlane

📢Did you know that researchers from around the world can apply for access to the latest microscopy technology & expert advice @aicjanelia.bsky.social?

In this blog series, the AIC team will give us an insight into how the Center works & their global impact.
focalplane.biologists.com/2025/11/20/a...

Chemical Tools for Complex Biological Systems IV Organizers Luke Lavis, HHMI/Janelia Research Campus Matthew Bogyo, Stanford University Zev Gartner, University of California, San Francisco Jennifer Prescher, University of California, Irvine

ahem, ONE of THE chemical biology conferences of 2026:) www.janelia.org/you-janelia/...

Another #FluorescenceFriday contribution for #Halloween a dive inside zebrafish vasculature

For #SpookyMicroscopyFriday I present the mugshot of the head of the zebrafish mafia

A microscopy image of a cell stained for actin (magneta color) and focal adhesions (green color).

Our team is hard at work preparing for #InsightFromImaging 2025! We can't wait to welcome HHMI #HannaGrayFellows to @hhmijanelia.bsky.social next week for lectures, hands-on labs, and activities focused on designing informative and quantitative bioimaging experiments 🔬

and the food...... It was raining like crazy the only time I went to the Monod conference but I still remember the food

For #MicroscopyMonday a cleared mouse embryos labeled with an antibody targeting neurofilaments (2H3, DSHB) @hhmijanelia.bsky.social @aicjanelia.bsky.social

Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning Optical nanoscopy of intact biological specimens has been transformed by recent advancements in hydrogel-based tissue clearing and expansion, enabling the imaging of cellular and subcellular structure...

for #FluorescenceFriday : is your ExM sample too large to be imaged with high NA objectives? VIPS it! Very clever way to tackle one of the biggest challenge in large volumetric ExM www.science.org/doi/10.1126/...

very cool! What imaging modality did you use to spy on these cells?

the scalewiew-A2 (25x) .95NA 8mmWD is probably your best bet right now. Between 2-3mmWD I go with Nikon for >1NA

The WB-ExM protocol described here works with.... every sample we tested! Here a 3do quail embryo (white= pan-protein; red=MF20) www.biorxiv.org/content/10.1...

Today was a super fun first day of imaging @aicjanelia.bsky.social with @vanderspuy.bsky.social and @samarar.bsky.social.
All hail the quail!🧪🔬#DevBio

Research Specialist - Tavakoli Lab Primary Work Address: 19700 Helix Drive, Ashburn, VA, 20147 Current HHMI Employees, click here to apply via your Workday account. The Howard Hughes Medical Institute’s Janelia Research Campus is a pio...

🧠 Excited by synapse-level brain mapping? Join us at a world-class institute to build molecular tools based on our new #LICONN work — and push the frontier of #MolecularConnectomics. Let’s decode the brain, one synapse at a time.

hhmi.wd1.myworkdayjobs.com/en-US/Extern...

Just over a week left to apply for this round of the AIC Call for Proposals! Submit at www.aicjanelia.org/apply by Monday, June 16 (5 pm US ET).

GEF25-Homepage - rizzoli-lab Program Location & Travel About Home GEF25, organized in the 10th anniversary year of ExM, focuses on cutting-edge advancements in expansion microscopy, as a part of global initiatives to democratize ...

Is this amazing ExM conference still happening? www.rizzoli-lab.de/gef25/

New technique expands tissues so hundreds of biomolecules can be seen inside cells A new tissue expansion method developed by Janelia and University of Wisconsin-Madison researchers enables scientists to use mass spectrometry imaging to simultaneously detect hundreds of molecules

🥼A new tissue expansion method developed by researchers in the Wang & Tillberg labs & collaborators @uwmadison.bsky.social enables scientists to use mass spectrometry imaging to simultaneously detect hundreds of molecules at the single cell level in their native locations ➡️ hhmi.news/4ixHOZZ

A highly stable monomeric red fluorescent protein for advanced microscopy - Nature Methods The red fluorescent protein mScarlet3-H is bright, photostable and very robust to high temperature, chaotropic conditions and oxidative environments. mScarlet3-H works well in correlative light and el...

I guess they renamed their protein from mYongHong to mScarlet3H www.nature.com/articles/s41...

A new call for proposals just opened with @aicjanelia.bsky.social . Do not miss this unique opportunity to collaborate with microscopy experts to advance your project!

Amazing opportunity!! #microscopy #bioImaging

Looks impressive! Check it out!