Find the full paper here: authors.elsevier.com/c/1mS0e5TA51...

👏 Big thank you to all co-authors (especially co-first authors Boya Wang and George Wright), reviewers/editors, colleagues, and institutions involved, especially at the NCI. Additional thanks to funding support from @dfg.de, Deutsche Krebshilfe and SFB 1530!

🚀 There is more to come building on these exciting findings and this data-rich resource.

-Expanding the epigenetic analysis and modeling GRNs present in normal and malignant B cells highlighted that transcriptional states of DLBCL genetic subtypes vary along three principal differentation. axes – GC B cell, memory B cell, and PC.

-Investigating the functional consequences of genetic alterations among the gen. subtypes revealed REL amp as a mechanism to block terminal memory B-cell diff.

-By integrating single-cell RNA and ATAC from tonsillar B cells, we learned gene-regulatory networks (GRN) defining normal B-cell states.

-Inference of DNA CNV revealed that each DLBCL tumor comprised up to 5 distinct malignant subclones.

-Analyzing the gene expression profiles of these subclones resulted in signature themes describing B-cell diff., cell proliferation, and cell growth that are distinctive across the subclones.

-DLBCL genetic subtypes differ strikingly regarding their tumor microenvironment.

-Gene expression in malignant B cells yielded predicted signatures for each DLBCL genetic subtype and highlighted their phenotypic diversity.

The genetic and gene expression subtypes of DLBCL have been defined using bulk tumor profiling. To study their biology in depth, we performed single-cell RNA and ATAC sequencing on 103 tumor biopsies. This revealed multiple fascinating findings:

First post here, but very happy to share our most recent findings from studying DLBCL at single-cell granularity (www.cell.com/cancer-cell/.... This was a great collaborative effort, and I am honored to have contributed as co-first author. Here's a short recap of the highlights. 🧵👇

This is amazing work! congrats🎉

This is such a cool work! Congrats 🎉