Really proud to have contributed to this paper! If you have the time, please, give it a read! 😊🌱 www.biorxiv.org/content/10.1...

Cell cycle follows pause and play mechanism in environment stress recovery in diverse plant species www.biorxiv.org/content/10.1101/2025.04....

(1/17) We are excited to share the Ashraf and Gallagher labs collaboration on cell cycle regulation during environmental stress recovery in Arabidopsis, Brachypodium, and ryegrass.

I'm so fortunate to be attending UBC and working in an awesome lab! 😆 Thanks so much for the endless support! yippee!

My student, Thea, got the @aspbofficial.bsky.social ASPB SURF for summer 2025 to work with us at @ubcbotany.bsky.social @ubcbotany.bsky.social UBC! She also got NSERC USRA this year! Thanks to Olivia @ohazel.bsky.social for mentoring Thea.

Congratulations 🍀🔬😊
What an achievement!

Fig. 1: Vacuoles respond to salt stress. Confocal images of root cells from Col-0 YFP-VAMP711 treated with 0 mM or 75 mM NaCl for 24 h, showing YFP fluorescence. (b’-b’’) Magnification of (Fig1.a), highlighting vacuole convolution in the medial plane. (c-c "”) 3D projections of YFP-VAMP711 at 0 mM (c") or 75 mM NaCl (c""), showing how a full reconstruction can mask vacuole convolution.

🧪🌱Preprint: "Regulation of vacuole fusion, a pivotal mechanism mitigating salt-induced inhibition of root cell growth"

It shows that low-salt stress inhibits vacuole fusion, but may serve as a protective mechanism!

📜Betz et al., 2025 dx.doi.org/10.21203/rs....

#PlantSciences

🔬☘️

Congratulations to Cecilia Rodriguez-Furlan's lab, @adrienheymans.bsky.social, and @srobertgroup.bsky.social for the recent preprint.

@ohazel.bsky.social and I are very grateful to contribute to the article from our lab.

www.researchsquare.com/article/rs-5...

oh my gosh!! so cute 🥺 if i had seen this in high school, i would have instantly decided to study biology in college haha

Thank you so much Dr. Fisher ☺️ yeah they're pretty great!

Thank you so much Min Ya! 😊 Yes, I'm looking forward to it!! 😆🌼

I am happy to share that I've began my PhD program with
@aribidopsis.bsky.social at @ubcbotany.bsky.social ! 😊🌱

thank you so much Héctor! 😄

thank you so much! 😊

thank you so much Minya!! That means a lot coming from you! 😄

the Ashraf Lab!!!

(5/5) – We would like to give a very special thanks to @minyaaa.bsky.social for inviting us to write for this special issue! We are also very happy and grateful that this paper was handled by Minako Ueda sensei!
Article: doi.org/10.1017/qpb....
Lab website: www.ashraflab.com/publications...

Proposed plant cell cycle marker line combining proAtPCNA1::AtPCNA1-mNeonGreen and proUBQ10::mScarlet-TUB6 to visualize distinct cell cycle stages (G1, early S, late S, G2, prophase, metaphase, anaphase and telophase) and mitotic structures

(4/5) – But there is still always room for improvement in plant cell cycle markers. We proposed the combination of two existing marker lines (PCNA-GFP and tubulin marker) to visualize all stages of the cell cycle, including each stage of mitosis.

Visualization of plant cell cycle marker lines (CYCB1;1-GUS, Cytrap, proAtPCNA1::AtPCNA1-sGFP and PlaCCI) throughout the distinct cell cycle stages (G1, early S, late S, G2, prophase, metaphase, anaphase and telophase).

(3/5) – Luckily, plant cell biologists have become very creative to circumvent the challenges of developing a FUCCI equivalent marker. This figure summarizes the four markers that have helped visualize each stage of the cell cycle so far. Feel free to use this illustration.

Graphical summary of cell cycle marker lines and each stage they visualize

(2/5) – You may already know that cell cycle marker lines have progressed much further in animals compared to plants. But why? It’s because in plants, there are challenges associated with developing a FUCCI equivalent marker. We go into more detail regarding this in the review.

(1/5) – I am very excited to share our publication in QPB with @aribidopsis.bsky.social ! Before working with Arif, I thought I knew at least the basics about the plant cell cycle, but shortly I realized that I knew almost nothing. If this sounds relatable at all, then this review is for you!

I have to participate (show off) - introducing my Mimulus mutant seedling #mimuluspropaganda

Another successful semester of organizing Microscopy Monday! This semester, the heavy lifting was done by Olivia @ohazel.bsky.social for co-organizing each sessions.

We are grateful to amazing speakers and cell biologists to contribute to the Microscopy Monday workshop series.

Yesterday was our last Microscopy Monday of the fall semester! We were very thankful and lucky to have
@fisherke.bsky.social lead a wonderful session covering considerations for electron microscopy 🔬🦠🩷
#Microscopy_Monday_Howard_University