Polard lab - Toulouse (@polardlab) Bsky

Pleased to share a collaorative study into the mode of action of the novel bacteriocin RumC1 - led in the lab by Anne Boyeldieu. This study reveals that RumC1 targets nascent peptidoglycan to kill monoderm bacteria.

www.biorxiv.org/content/10.6...

1 month ago 2 2 0 0

Two D-loop resolution systems enable natural genetic transformation in bacteria Natural transformation is a widespread mechanism driving genetic exchanges in bacteria. It proceeds by the capture and internalization of exogenous DNA in linear single strands, ultimately integrated in the genome by homologous recombination. It is unknown how the RecA-directed D-loop intermediate of this dedicated recombination pathway is processed. We report that resolution of the transformation D-loop depends on two endonucleases of opposing phylogenetic distribution in bacteria. One is YraN, which has co-evolved and interacts with the ComM helicase, known to extend DNA recombination at the transformation D-loop. The other is CoiA, which is restricted to the Bacillota. CoiA is shown to be a resolvase of the transformation D-loop, extended by the RadA helicase in these species. We demonstrate that both YraN and CoiA act synergistically with their cognate helicases. These findings reveal that bacteria have evolved two helicase/nuclease pairs for the maturation and recombination extension of the transformation D-loop. ### Competing Interest Statement The authors have declared no competing interest. Agence Nationale de la Recherche, https://ror.org/00rbzpz17, ANR-20-CE12-0004, ANR-10-BLAN-1331, ANR-17-CE13-0031, ANR-22-CE44-0044, ANR-10-LABX-62-IBEID, PIA/ANR-16-CONV-0005 Fondation pour la Recherche Médicale, https://ror.org/04w6kn183, FDT202001010890 European Union's Horizon research and innovation programme. Marie Skłodowska-Curie Postdoctoral Fellowships, 101208987 National Institute of Health, USA, R35GM128674

#microsky
Massive update of preprint with @polardlab.bsky.social!
Bacteria have evolved two systems to recombine extracellular DNA
www.biorxiv.org/content/10.1...
Kudos to lead authors Léo Hardy, Violette Morales and Clothilde Rousseau, and to outstanding Dalia's lab and @epcrocha.bsky.social
🧵⬇️

3 months ago 18 8 1 0

A strong and fruitful collaboration with Patricia Bordes in the team of @genevauxpierre.bsky.social

3 months ago 1 0 0 0

A toxin/antitoxin system targeting the replication sliding-clamp induces competence in Streptococcus pneumoniae Author summary The environment in which bacteria live puts them under a great deal of stress, forcing them to adapt constantly, either temporarily or permanently. Streptococcus pneumoniae, a pathogeni...

Delighted to share a new paper from our lab, a study led by Mathieu Bergé looking at how an endogenous toxin targets replication to induce competence in the pneumococcus. dx.plos.org/10.1371/jour...

3 months ago 15 10 1 1

New paper from our lab up on Biorxiv, showing how a toxin targets replication to induce competence. 👇

7 months ago 2 3 0 0

Here's a new preprint from our lab on the mechanism of DNA uptake during transformation. Collaboration with Guerois and Radicella labs.

11 months ago 4 3 0 0

Happy to share a recent paper from our lab in collaboration with Rémi and his team 👇

1 year ago 0 0 0 0

Hello Bluesky! As a first post on this platform, we are happy to share the latest paper from our lab exporing links between competence, homologous recombination and genotoxic stress.

Please like/repost/follow to get us going on this platform!

1 year ago 5 3 0 0

Posts by Polard lab - Toulouse