For more details, check out the chapter 👇
onlinelibrary.wiley.com/doi/10.1002/...

And to see the method in action 👇
www.science.org/doi/10.1126/...

We then pushed it further
🦠 Tracking M. marinum-induced damage in Dicty
🧫 Monitoring Gal3 recruitment after LLOMe in mammalian cells

As a proof of concept ✨
We used Dictyostelium cells expressing two ESCRT reporters: Alix & Vps32.
→ Our approach tracks their kinetics after LLOMe-induced damage and confirms earlier recruitment of Alix after damage

To monitor membrane damage & repair in real time, we developed a robust high-throughput approach combining:
📸 live high-content imaging
📊 automated quantification
🧬 stable, homogeneous reporter cell lines

🧵 Tiny organisms, big advantages!

Ca²⁺ leakage is a conserved signal for non-canonical ATG8/LC3 lipidation and membrane repair - The EMBO Journal Endomembrane damage of intracellular vesicles triggers signals that activate membrane repair in mammalian cells to restore homeostasis. However, the signals that drive diverse membrane repair recruitm...

💥Our latest @embojournal.org on membrane damage and repair. Di @dichen02.bsky.social @crick.ac.uk found that calcium is a conserved signal for ATG8/LC3 lipidation on membranes using novel probes to record calcium leakage. Congratulations Di et al!
link.springer.com/article/10.1...

Very excited to share our recently published work on NTM and their response to acid stress!

As my first paper, I’m especially grateful for the support and guidance from @luizcarvalholab.bsky.social , Rachel and @maxgg.bsky.social . More to come!

🚨 New preprint!

Together with @carobarisch.bsky.social lab, we investigated how intracellular compartmentalization shapes lipid acquisition in mycobacteria

Interestingly, deleting one FACL enzyme (FACL6) revealed striking compartment-dependent phenotypes 🔬

Check it out: doi.org/10.64898/202...

🎉 Big news! Last Saturday, Thierry was awarded the Egon Naef Foundation Prize for in vitro research 🏆 A wonderful recognition of his innovative work throughout his career developing alternatives to animal testing 👏

www.unige.ch/sciences/chi...

@sciencesunige.bsky.social

🚨 Preprint alert 🚨

In this study, we reveal how a calcium-sensing protein coordinates membrane damage sensing with repair pathways in response to damage from intracellular mycobacteria

Huge congrats to @guallar-garrido.bsky.social for leading this project! 🤩👏

www.biorxiv.org/content/10.6...

Thanks a lot Pierre ☺️✨

Membrane microdomains are crucial for Mycobacterium marinum EsxA-dependent membrane damage, escape to the cytosol, and infection Sterol-rich microdomain accumulation at the MCV is crucial for Mm-induced damage and infection in D. discoideum and BV-2 cells.

🚨 Want to know how we demonstrated the importance of sterol-rich membrane microdomains for M. marinum infection?

Check out our latest paper published in Science Advances 👇
www.science.org/doi/10.1126/...

✨ Big thanks to everyone, especially Cristina Boehm-Bosmani, @soldatilab.bsky.social ! ✨

New paper out! 🚨

We uncover how Mycobacterium marinum (red) exploits host sterol-rich membrane microdomains (green) to damage and escape from its phagosome

Huge shoutout to @angieprrt.bsky.social and Cristina Bosmani for their very elegant work! 👏
www.science.org/doi/10.1126/...

Thank you ☺️

FEBS Press CRISPRI-mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI-med...

🚀 Latest paper form the lab is now out at @febsopenbio.bsky.social
@febspress.bsky.social 🔵⚪🔴

We use CRISPRi tools from the @rocklabtb.bsky.social to target essential genes and control smooth to rough transition in M.smegmatis & M.abscessus 🦠🧫👇👇👇

febs.onlinelibrary.wiley.com/doi/10.1002/...

Really honoured and grateful to have received the Laemmli Prize ✨ A wonderful recognition of my PhD work @sciencesunige.bsky.social in the @soldatilab.bsky.social

🏆 It's a double win for the Soldati Lab! Last Friday, our former PhD students @angieprrt.bsky.social and Jahn Nitschke each received an award for their doctoral work: the Laemmli Prize for Angélique and Best Thesis in Life Sciences for Jahn. So proud of both for these well-deserved honors! 🤩

Molecular basis for cellular compartmentalization by an ancient membrane fission mechanism The emergence of cell compartmentalization depends on membrane fission to create the endomembrane compartments. In eukaryotes, membrane fission is commonly executed by ESCRT-III, a protein complex con...

New preprint from the lab!!🎉
We show that Asgard archaea ESCRT-III proteins can trigger membrane fission and reveal its molecular mechanism, offering clues to how these cells may have built internal compartments. But do these organisms even have these compartments?
www.biorxiv.org/content/10.1...

Why would Mycobacterium shed its surface right after entering a host cell? Host phagocytes rapidly strip its outer layer, releasing components that alert neighbors and trigger defense responses

Our preprint is out, curious to hear your take! 👀
www.biorxiv.org/content/10.1...

Yeast cells utilize a TORC2 feedback loop for plasma membrane adaptation to mechanical stress, with increased sterol transport from plasma membrane to endoplasmic reticulum activating TORC2 signaling
Robbie Loewith, Aurélien Roux @rouxlab.bsky.social and coworkers
www.embopress.org/doi/full/10....

🇻🇳 What a great week at the BPHCI Conference in Vietnam! @j-toinon.bsky.social and @angieprrt.bsky.social enjoyed sharing their results on M. marinum–Dicty interactions. A small crowd, but packed with brilliant science and great vibes! 🥂

Frequency dependence of fast-lifetime FLIM and fits for C2C12 cells.

Fluorescence lifetime estimation: a practical approach using Flipper-TR FLIM by Juan Manuel Garcia-Arcos and team: www.biorxiv.org/content/10.1...

Inorganic sulfate is critical for Mycobacterium tuberculosis lung tissue colonization and redox balance | PNAS Tuberculosis remains the deadliest infectious disease caused by a single pathogen, highlighting the urgent need for novel therapies. A deeper under...

Excited to share Wendy Le Mouëllic’s PhD work, now published in @pnas.org!
It reveals that M. tuberculosis depends on inorganic sulfate import to survive inside host cells—fueling essential processes such as redox balance and stress resistance.
Huge congrats to Wendy & colleagues!
shorturl.at/WbFQC

Hello microscopy and imaging experts and enthusiasts!
We are looking for a staff scientist to join us here in Geneva to work on microscopy and image analysis projects! See the link for the job announcement (in French and in English).

Please apply and/or repost!

jobs.unige.ch/www/wd_porta...

Merci 😊

Paramoeba atlantica as a Natural Intracellular Niche for Vibrios in Marine Ecosystems Various vibrios can colonise a large vacuole called VCV (Vibrio-containing-vacuole) within Paramoeba atlantica. The VCVs acidify and the vibrios multiply and remain alive inside the VCVs for hours. T...

Our last paper just came out ! A great piece of work from the talented Laura Onillon and teammates with the pleasure to share this adventure with friendly collaborators @vincentdlft.bsky.social and @soldatilab.bsky.social !

Structural dissection of ergosterol metabolism reveals a pathway optimized for membrane phase separation The metabolism of ergosterol shapes this lipid to allow the formation of fluid membrane domains in yeast cells.

Out today, we report how small chemical modifications in the metabolism of ergosterol are needed for yeast membranes to phase seperate into ordered yet fluid domains www.science.org/doi/10.1126/... (1/n)

Paramoeba atlantica as a Natural Intracellular Niche for Vibrios in Marine Ecosystems Various vibrios can colonise a large vacuole called VCV (Vibrio-containing-vacuole) within Paramoeba atlantica. The VCVs acidify and the vibrios multiply and remain alive inside the VCVs for hours. T....

🚨 Great story released by @gm-charriere.bsky.social and co.!
They identified and characterised a natural interaction between amoebae and Vibrio, pointing to Paramoeba as a potential reservoir of oyster pathogens 🦪
Congrats! 🥂
enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/...

🤩 Last week I successfully defended my PhD!
It was such a special day, made even better by having friends, family, and colleagues there with me. Thanks for all the support and kind words!
Huge thanks to my jury members for the thoughtful feedback and conversations.

@soldatilab.bsky.social

Did you know the cell wall of mycobacteria isn’t just for protection? 🧱👀 Here, Mycobacterium marinum sheds its outer capsule right after being eaten by Dicty! A sneaky move that affects even nearby cells… #FluorescenceFriday #Mycobacteria #CellBiology #Infection #3Rmodels #Phagocytosis