Thanks Tanja! I would think it is similar in humans based on the cryo-EM structures. What have you tried to capture hTAF2 on promoters?

High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo TFIID is instrumental in recognizing promoter sequences and initiating transcription, yet a cohesive understanding of how this complex interacts with and functions at different promoter types in vivo ...

The @zeitlingerlab.bsky.social is pleased to announce @sergio-gma91.bsky.social’s preprint “High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo” (www.biorxiv.org/content/10.6...).

TLDR; TFIID behaves differently depending on promoter type. More below:

Widespread low-affinity motifs enhance chromatin accessibility and regulatory potential in mESCs Low-affinity transcription factor (TF) motifs are an important element of the cis-regulatory code, yet they are notoriously difficult to map and mechanistically incompletely understood, limiting our a...

We are pleased to announce a new preprint by @mlweilert.bsky.social: “Widespread low-affinity motifs enhance chromatin accessibility and regulatory potential in mESCs” (www.biorxiv.org/content/10.1...). See summary and longer recap below:

(TLDR; low-affinity motifs matter as pioneers!)

High-resolution binding data of TFIID and cofactors show promoter-specific differences in vivo www.biorxiv.org/content/10.64898/2025.12...