Felix Pahmeier (@fpahmeier) Bsky

Dengue Virus NS1 Binds Ephrin B1 to Trigger Endothelial Dysfunction Dengue virus (DENV), a member of the Flaviviridae family, is the most prevalent and medically important mosquito-borne viral pathogen. Infected cells secrete the viral non-structural protein 1 (NS1) into the bloodstream, where it can interact with endothelial cells to induce vascular leak. Host factors involved in NS1-mediated endothelial dysfunction are incompletely understood, leading us to investigate the host interactome of DENV NS1 in endothelial cells via a comparative mass spectrometry approach. We identified ephrin B1 (EFNB1) as a critical host factor in NS1-mediated endothelial barrier dysfunction and show that phosphorylation of EFNB1 is necessary for induction of barrier dysfunction. Further, we map the interface of the EFNB1-NS1 complex through biochemical and computational approaches, and we show that EFNB1-Fc-fusion proteins can act as decoys to block NS1-induced barrier dysfunction in vitro and in vivo. This study provides insights into the mechanism of flavivirus NS1-mediated endothelial barrier dysfunction and new avenues to target vascular leak.

Dengue Virus NS1 Binds Ephrin B1 to Trigger Endothelial Dysfunction bioRxivpreprint

5 months ago 0 1 0 0

Check out our latest study, led by grad student Ryan McCool @rmccool.bsky.social‬, in collaboration with Vaccine Company. We were able to stabilize EBV gB in the prefusion conformation, determine its cryo-EM structure to 3.1 A, and assess its immunogenicity in mice. www.biorxiv.org/cgi/content/...

11 months ago 60 20 1 1

Antibodies targeting Crimean-Congo hemorrhagic fever virus GP38 limit vascular leak and viral spread Glycoprotein GP38-specific antibodies reduce viral dissemination and GP38-mediated vascular leak in Crimean-Congo hemorrhagic fever virus infection.

Happy to finally see this study published, a great collaboration between academic, government and industry labs! We show that CCHFV GP38 can induce vascular leak and that this can be prevented by protective but not virus-neutralizing antibodies! www.science.org/doi/10.1126/...

1 year ago 9 5 0 0

Engineering and structures of Crimean-Congo hemorrhagic fever virus glycoprotein complexes Crimean-Congo hemorrhagic fever virus is a widespread tickborne virus that infects humans. Engineering-based structural studies of the viral glycoprotein complexes GP38-Gn and GP38-Gn-Gc provide insights to their function in the viral life cycle. GP38-Gn-Gc confers partial protection against lethal viral challenge in mice, showing promise as a vaccine antigen for further development.

I'm happy to announce that our paper is now online in Cell! This study was the result of a highly collaborative effort from many scientists. Please read it here: www.cell.com/cell/fulltex...
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1 year ago 16 11 1 1

A trivalent mucosal vaccine encoding phylogenetically inferred ancestral RBD sequences confers pan-Sarbecovirus protection in mice The continued emergence of SARS-CoV-2 variants and the threat of future Sarbecovirus zoonoses have spurred the design of vaccines that can induce broa…

"A trivalent mucosal vaccine encoding phylogenetically inferred ancestral RBD sequences confers pan-Sarbecovirus protection in mice"

www.sciencedirect.com/science/arti...

1 year ago 16 7 0 0

Posts by Felix Pahmeier