I illustrated my way through the @bsdb.bsky.social Spring Meeting a couple weeks ago - here's my illustrated summary of the conference! I sketched (almost) all the speakers and glimpses of the science that made it such a great few days.
Amazing!
Specification of tuberal neurogenic progenitors at early neural plate stages (A–I) Maximum intensity projections of hemi-views (A-C, D-H') or ventral view of isolated neuroectoderm (I) of HH6 (A-C, I) or HH17–18 (D–H') embryos after HCR. (A–C) Hemi-views labelled for SHH and NKX2-1 (A); SHH and PTCH1 (B); FOXG1 and BMP2 (C). White bracket marks HypFP cells. (D-E') Fate mapping of HypFP cells at HH6. (D) Mid-HypFP cells (dorsal view, inset in D) give rise to tuberal gliogenic cells, identified by their position relative to the mammillary pouch (mp arrowhead in D). (E) HH17 embryo after anterior-most HypFP cells labelled at HH6 with DiI, and HCR analysis. DiI is located in the SHH+ neurogenic hypothalamus, the SHH- gliogenic hypothalamus, but not the FOXG1+ telencephalon. Boxed regions in (F, G) shown at high power in (F′, G′). (H′) shows double channel view of FGF10 and FOXA2. Arrowhead points to mammillary pouch (mp) and optic stalk (os). (I) HH6 isolated neuroectoderm after HCR to detect SHH/PTCH1. White bracket marks HypFP cells; white lines represent positions of slices shown in I’. (I′) Explants of regions 1–4, after HCR to detect SHH/PTCH1 confirms accuracy of dissection. (J) Midline sub-dissection from a position 4 explant (yellow boxed region in I′), showing position of DiI-labelling (red circles). MIP shows SHH and PTCH1 are expressed through the explant at 0 h. (K–Q) MIPs of wholemount views of HH6 Hyp explants (regions 3 or 4 in (I′)), cultured for 72 h, then analysed by HCR (right hand panels show single or double channel views) showing expression of SHH/PTCH (L); NKX2-1/POMC/FOXA2 (M); SHH/PTCH1/NKX2-1 (N); ISL1/POMC (O); FGF10 (P); TBX2 (Q). Scale bar = 100 μM.
#DBfeature 🧠
FGF10 operates with SHH and BMP to contribute to neurogenic and gliogenic fates in the puberal hypothalamus
By K Chinnaiya, I Groves, E Manning, E Place, C Furley, SE Strawbridge, AG Fletcher, M Placzek
tinyurl.com/muee82sm
Government-Funded infomercial from Norway striking back at US corporations and the tech Bros for filling the Internet with slop.
youtu.be/T4Upf_B9RLQ?...
Excited to finally share the final/final.doc version of our paper. It's been a journey, but very proud of the result. Well done to all involved, especially @elsieplace.bsky.social
@kchinnaiya.bsky.social , @thomasdwkim.bsky.social, @sethblackshaw.bsky.social 👏👏
www.nature.com/articles/s41...
Very happy to share our latest article in @natcomms.nature.com !
Also very pleased to be a featured article (beautiful artwork from @elsieplace.bsky.social depicting early chick neurectoderms at increasing stages of development. (waiting patiently in space for the right moment to attack the Earth?)
A very good, entertaining and frankly terrifying look at neural networks and AI
www.youtube.com/watch?v=jrK3...
“Developmental biology is fundamentally beautiful. We are no less beautiful for our variation. Instead, perhaps we are more so. Perhaps we are remarkable. Perhaps we are full of wonder.”
Read this insightful post by Bethan on the Node.
thenode.biologists.com/developmenta...
#Disability
Movie S4
Movie S3
Movie S2
Movie S1.2: Temporal progression from HH stage 10 to HH Stage 20 (multi angle).
Movie S1: Fate map of HH Stage 10 to HH Stage 20 chick neuroepithelium
Our preprint (www.biorxiv.org/content/10.1...) describes how we have fate mapped the chick neural tube and created the most complete forebrain fate map to date. In this thread, I’ll post our movies describing forebrain morphogenesis
Looking forward to the #biologists100 conference in Liverpool next week!
I’m creating a thread below to link to my poster (in collaboration with @elsieplace.bsky.social) so we can share our 4D model of forebrain development.
Come visit us: Poster 200, Session 2 on Wednesday.
A still image of a stage HH4 gastrulating chick embryo electroporated with 3 different fluorescent reporter genes shown in magenta, yellow and cyan.
Need to electroporate and/or live image avian embryos? We hope our new preprint will help you get started:
www.biorxiv.org/content/10.1...
Open EGGbox: an open-source 3D-printed embryonic Gallus gallus toolbox for electroporation and culture/live imaging of avian embryos ex ovo
🧪🐣 #devbio
This is a profoundly evil betrayal and a direct reflection of Trump's soul—a disgusting, small, and shameful ghoul who gets off on harming the powerless.
www.reuters.com/world/us/tru...
Planar polarity in the Drosophila pupal wing is oriented by tissue stress but the mechanisms remain unclear. This study uncovers a crucial role for cell flow gradients in promoting planar polarity protein turnover and aligning proximodistal polarity.
New! 15y after the classic Aigouy et al "Cell Flow Reorients the Axis of Planar Polarity in the Wing Epithelium..." from lab of much missed Suzanne Eaton, we provide our take from post-doc Sara Tan "Tissue shear as a cue for aligning planar polarity in the developing Drosophila wing".
rdcu.be/d81Vn
Sunrise over Sheffield
sneak preview of our animation...
Brought to life through the magic of Blender...
For more details and more movies go to our preprint
www.biorxiv.org/content/10.1...
Check out our fabulous 4D model of forebrain development, created in Blender from imported light sheet scans of HH10-20 dissected chick neuroepithelium, and painstakingly linked together by @elsieplace.bsky.social by combining published fate maps with 100's of our own samples.
Excited to present our new preprint clarifying forebrain developmental organisation. Complex growth patterns shape the forebrain but distort the A-P + D-V axes, hence prior confusion over brain layout. Big love for chicken embryos, simpler to interpret than mouse 🐣1/3 www.biorxiv.org/content/10.1...
Great work from @thomasdwkim.bsky.social and @sethblackshaw.bsky.social labs
I haven't seen that experiment - I'll have to look it up. Thanks!
Phenomenal effort by @elsieplace.bsky.social for her fist (of many) last author paper!
We begin the New Year with two preprints on hypothalamic development. The first, part of a long-term collaboration with Marysia Placzek’s group, investigates the organization of the developing forebrain, and show that current models are (mostly) incorrect./1
www.biorxiv.org/content/10.1...
Excited to share our new preprint:
