🌀 How do spirochete bacteria swim through thick fluids like champions?

We solved T. denticola flagella structure - asymmetric proteins expand one side, compress the other for perfect corkscrew motion!

@debnathghosal.bsky.social

🔗 doi.org/10.64898/202...

#StructuralBiology #CryoEM #Microbiology

📣 New paper alert! This is a story of molecular tricks that let spirochetes (spiral-shaped bacteria) drill through tissue. www.biorxiv.org/content/10.6...

Wonderful spirochete asymmetric flagellar structural work by @lucatroman.bsky.social Grateful to be part of the story supported by @debnathghosal.bsky.social

I noticed something similar, especially with membrane proteins. Dynamo worked fine, but I always got strange reconstructions with RELION.

An Asgard archaeon from a modern analog of ancient microbial mats It has been proposed that eukaryotic cells evolved via symbiosis between sulfate-reducing bacteria and hydrogen-producing archaea. Here we describe a highly enriched culture of a novel Asgard archaeon...

📣 New paper alert!
One of the most exciting projects we've done in recent years is now out on BioRxiv: "An Asgard archaeon from a modern analog of ancient microbial mats".

Glimpse into early complex life! ❄️🦠 🔬🧬

www.biorxiv.org/content/10.1.... A thread...

End-to-end localized deep learning for Cryo-ET Cryo-electron tomography (cryo-ET) enables 3D visualization of cellular environments. Accurate reconstruction of high-resolution volumes is complicated by the very low signal-to-noise ratio and a rest...

I have been using CryoLithe recently as DeepDeWedge and cryoCARE erase many of the periplasmic complexes that I want to pick.
arxiv.org/abs/2501.15246

If you are unfortunate and have to manually pick particles. I have had the most success using the WARP deconvolution filter.

My total reliance on Nvidia GPUs is a bit concerning.

Has anyone encountered the issue of DM3 crashing and taking TIA with it during long tomo collections? If so, did you find a solution?

#Teamtomo #CryoET

It seems like, no matter what technique you use, it's Fourier transforms all the way down.

For tomography and STA, I feel like EMAN2 was ahead of its time. I am still confused as to why it isn't used by more people.