Pre-run FPLC checks YouTube video by the bumbling biochemist

Before you press start, here are some things to check on your AKTA (or other brand of FPLC) youtube.com/shorts/wJbu3...

Scientific research posters – what they are & advice for preparing and presenting them Research poster guidance https://www.youtube.com/watch?v=24qtTQCS5NM https://youtu.be/24qtTQCS5NM Posters are one of the main ways in which scientists (often scientists in training) present “ho...

Here's a guide I hope can help people make sense of things and prepare their own awesome posters! thebumblingbiochemist.com/365-days-of-...

Scientific research posters – what they are & advice for preparing and presenting them YouTube video by the bumbling biochemist

Research posters and their presentations can be another one of those things students all too often seem to be expected to know how to do. And, complicating matters, there's no one "right way" to make them. . . youtu.be/24qtTQCS5NM

Batch binding with gravity flow chromatography As for the unsticking, here too I like to take advantage of the gravity flow format to try to get my protein to come off in a more concentrated form (though I do want to try seeing if I can recreate s...

thebumblingbiochemist.com/365-days-of-...

Batch binding with gravity flow chromatography YouTube video by the bumbling biochemist

I love FPLC (Fast Protein Liquid Chromatography) - but sometimes, I still feel gravity flow is the way to go! I often prefer to do affinity chromatography using good ‘ole gravity flow (with batch binding which I’ll talk about below) before moving on to the fancy machine. youtu.be/1PN90h5o_3U

Today my mom got to meet part of the Bibel lab and see me in my element – Huge thanks to Liam, Ryan, Korina, & Brooke for coming to hang with us and to all my colleagues who took time to visit and help my mom see how supported I am here. I’m so grateful to have landed at LMU!

Gravity flow was definitely the way to go . . . we have an impractically large protein yield! Next week - figuring out how to run SEC on 90 mg of protein . . .

Yesterday was purification day - the old school way! (which I sometimes think works better for affinity anyway...)

Satellite colonies with ampicillin selection YouTube video by the bumbling biochemist

Satellite colonies are bacterial free loaders - they’re tagging along for the antibiotic selection ride even though they don’t have our plasmid inside!  youtu.be/xENBDYsdFBQ bit.ly/satellitecol...

bit.ly/nativepageov...

Blue Native PAGE YouTube video by the bumbling biochemist

When's it good to feel blue? When you're running a blue native PAGE! youtu.be/T0eYmTDX1bc

Got an AKTA loop valve :) YouTube video by the bumbling biochemist

We're quite excited about getting a loop valve for our AKTA so we can have multiple sample loops hooked up at once youtube.com/shorts/RIu0O...

Does anyone know of anyone who might be able to give a meeting talk about the environmental costs of AI, ideally in the context of education &/or biochemistry? Thanks in advance

More here: bit.ly/biologics_etc & bit.ly/antibodytype...

Biomolecular conjugates YouTube video by the bumbling biochemist

Take cool molecule 1 and chemically glue it cool molecule 2. Now you've got a single conjugate molecule combining the coolness of the 2! youtu.be/ArW3B4pgY2k

SDS-PAGE - the protein ruler to rule them all I showed you how we could use X-ray crystallography to “look” at proteins at the atomic level, where we can see how the different carbon, nitrogen, hydrogen, oxygen, and sometimes sulfur atoms link to...

And bit.ly/sdspageruler

more: bit.ly/proteinclean..., bit.ly/gel_vs_blot

Interpreting SDS-PAGE gels & using them to assess protein purification scheme efficiency & efficacy YouTube video by the bumbling biochemist

Interpreting SDS-PAGE gels & using them to assess protein purification scheme efficiency & efficacy youtu.be/ifDJUt8Qz7I

bit.ly/cbbgelstaining

Colloidal Coomassie Brilliant Blue stains – the way "Instant" protein gel stains work YouTube video by the bumbling biochemist

Colloidal Coomassie Brilliant Blue stains – the way "Instant" protein gel stains work youtu.be/WlFrYxqGD64

More random practical lab tips & tricks: bit.ly/lab_tricks_p...

Tips for getting a publication-pretty PAGE (SDS-PAGE, urea-PAGE, etc.) youtu.be/8MCfiSiJDNU

PAGE tip – use a gel loading tip to straighten well walls before loading your samples YouTube video by the bumbling biochemist

PAGE tip – use a gel loading tip to straighten well walls before loading your samples. This will help your lanes run unwarped and ensure you are able to get your tip in the well without disrupting the neighboring ones and/or having your sample jump out.

youtube.com/shorts/a7waW...

Preventing leaks in SDS-PAGE (emphasis on setting up Biorad TetraCell cassette modules) YouTube video by the bumbling biochemist

Preventing leaks in SDS-PAGE (emphasis on setting up Biorad TetraCell cassette modules) youtube.com/shorts/kS0rH...

Cool chemical crosslinking gel (protein-protein with DSS) – explained by Alice very well! YouTube video by the bumbling biochemist

Cool chemical crosslinking gel (protein-protein with DSS) – explained by Alice very well! youtube.com/shorts/xXvB0...

Peeps demonstrating the ideal gas laws Happy Easter peeps! Did you know Peeps are ideal for demonstrating ideal gas laws? https://www.youtube.com/watch?v=KE7RTXz4Piw https://youtu.be/KE7RTXz4Piw https://www.youtube.com/watch?v=rkQ...

bit.ly/peepsidealga...

Peeps Ideal Gas Law demo YouTube video by the bumbling biochemist

Looking for something to do with your Peeps? Might I suggest demonstrating ideal gas laws?  youtu.be/KE7RTXz4Piw

Book rec: My life, my science: Pursuing a Cure for Huntington's Disease by Nancy Sabin Wexler YouTube video by the bumbling biochemist

New (literally!) book recommendation: My life, my science: Pursuing a Cure for Huntington's Disease by Nancy Sabin Wexler (in collaboration with Mark Hampton and Alice R. Wexler), 2026
youtu.be/sgB5If8WyJE

Leavening agents & fermentation Why’s matzoh flat, but matzoh balls are fluffy? Fluffiness comes from gas generation in dough that causes it to expand (leaven), which can come from biological fermenters (yeast) or chemical leavening...

Matzoh is cooked before yeast have a chance to leaven it. This kills the yeast so they can’t ferment any more, but if you crush that matzoh up into “matzoh meal” you can make a dough with that and you can make it fluffy with chemicals (baking soda) instead of yeast. bit.ly/leaveningage...