A comprehensive review on covalent chemical strategies for protein self-labeling from @kjohnsson.bsky.social's lab: HaloTag, SNAP-tag et al. www.annualreviews.org/content/jour...

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Bluetorial: Mentors: The long-serving dean

Art Levine was a great leader, first at NIH, and then at the University of Pittsburgh

He is a great mentor to me and others and a scientist's scientist

(A long palate cleanser for today)

🎉 30 Years of Single Molecule Science🎉 Join us for the 30th Single Molecule Workshop with Nobel Laureates Stefan Hell and W. E. Moerner. Present your research and connect.
📅 Abstracts: April 16, 2025
📅 Workshop: Sept 23–26, 2025 | Berlin
🔗 www.single-molecules.org

#️⃣ #singlemolecules #microscopy

A wonderful meeting to share the latest developments on single-molecule research, network, and generate new ideas! Great invited speakers @sulianamanley.bsky.social @jungmannlab.bsky.social, David Rueda, Sebastian Diendl! Send us your Abstracts by *March 7th*!

Thank you to the fantastic speakers who presented today at our first meeting!
We are now accepting submissions for our second meeting on April 7th.

Submission link : forms.gle/SBeQeAp9MR2U...

Here's a set of three for #FluorescenceFriday. Same image, progressively higher zoom. FFPE human tonsil tissue labeled with primary antibodies against Cytokeratin, PCNA, C20, CD45RB, CD68, eCadherin, and SMA. 🧪🔬

Every scientist, long before they were a scientist, was a little kid who stood in front of a fish tank or stared up at the stars or turned over a rock to look at the bugs underneath and said “wow”. On the best days, working in science still feels like that.

DNA-PAINT imaging with hydrogel imprinting and clearing www.biorxiv.org/content/10.1101/2025.02....

Congrats- just added to my reading list!

Improvements in LM (top) and EM (bottom) of our micro-sam model (finetuned) compared to the default SAM model.

After a long journey, Segment Anything for Microscopy is now published in Nature Methods! We significantly improve SAM for interactive and automatic segmentation in light and electron microscopy and build a user-friendly tool.
www.nature.com/articles/s41...

Wonderful piece of work- congratulations!

ÅNGSTRÖM-RESOLUTION IMAGING OF CELL-SURFACE GLYCANS 🧬🎨🍬

The glycocalyx, our cells' sugar coat, holds secrets in immunology, cancer, viral infections, and more. Visualizing its molecular architecture was impossible… until now. #glycotime #microscopy

www.biorxiv.org/content/10.1...

For those of you running system quality control measurements: New publication from QUAREP-LiMi for illumination power, stability and linearity measurements
www.protocols.io/view/illumin...

Cutting unfunded research ‘hits Newcastle Russell Group standing’ Questions raised about institution’s research reputation as it seeks to reduce academics’ research hours to plug £20 million black hole

More details on Newcastle University redundancies. Of the 300 FTEs to be lost, a proposed 50/50 split between academic and administrative posts. This is 5% of the workforce. 40 posts to be lost from Academic Hub (student study skills and advice). 1/3

Highly multiplexed spatial transcriptomics in bacteria Single-cell decisions made in complex environments underlie many bacterial phenomena. Image-based transcriptomics approaches offer an avenue to study such behaviors, yet these approaches have been hin...

🅲🅾🅾🅻 🆃🅴🅲🅷🅽🅾🅻🅾🅶🆈

“Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo

Super resolution confocal image of a lateral line neuromast in a 5-day-old zebrafish - it looks like an orange and purple flower with green dots

Super resolution confocal image of a lateral line neuromast in a 5-day-old zebrafish 🐟
The green dots are amazing specialized presynaptic structures called synaptic ribbons 🎀
Hair cell actin labeled in orange, acetylated tubulin in neurons & hair cells labeled in purple #FluorescenceFriday

I’ve said “right” about 10 separate times this morning in a bid to get myself going. It’s the British person version of repeatedly yanking the starter cord on an old lawnmower.

A pair of COS cells, one in interphase and one in mitosis for
#FluorescenceFriday. Tricky 4-channel overlay made possible by @kwolbachia.bsky.social LUTs! Microtubules 🟣 vimentin 🟢 mitochondria 🟠 clathrin 🔵, deconvolved Apotome.

MPicker: visualizing and picking membrane proteins for cryo-electron tomography - Nature Communications Visualization and picking of membrane proteins in cryoET tomogram is often challenging. Here, the authors developed a software MPicker based on membrane flattening to simplify the visualization, picki...

Paper of interest for the #CryoET folks.

www.nature.com/articles/s41...

The cover of Biophysical Reports, showing an influenza virus particle partially disassembling to release lipid molecules and expose the interior

Delighted to hear that Andrew McMahon and Nicole Robb's study 'Engineering stress as a motivation for filamentous virus morphology,' which we and other @cvrinfo.bsky.social authors collaborated in, is now a beautiful cover image. You can read the paper here: www.sciencedirect.com/science/arti...

a cartoon says hey everybody an old man 's talking while bart simpson looks on ALT: a cartoon says hey everybody an old man 's talking while bart simpson looks on

Bluesky: Revising my PhD thesis, 41 years later.

Just before the holidays, I discovered a paper I had missed (from 25 years ago) that showed that a major interpretation from my dissertation was wrong. I now have things sorted and the results are quite interesting, maybe better than the original.

May your holidays sparkle as much as Alexa647 💖

Britain has seen an alarming rise in poetry sales --The Economist, 15 Jan 2024

2024 Headline of the Year nominee (January)

The 2025 EMBL Annual Poster, listing all of the courses and conferences taking place in 2025 at EMBL

Hi Bluesky community! 👋

Are you looking forward to 2025 as much as we are? 👀

We just updated our 2025 Annual Poster to include even more events for you to join, so go and have a look!

➡️ s.embl.org/poster-bl

#EMBLEvents #molecularbiology #lifesciencetraining

Line of zebrabow embryos that have different colors based on the cell lineage. They are sorted by color to form a rainbow.

Full spectrum of ‘zebrabow’ embryos. Credit to @jhenninger.bsky.social. #ZebrafishZunday 🧪

A purple and green frog embryo. The green is labeling hair cells, while the purple is labeling musculature near the spine.

For today's #FluorescenceFriday it's a frog embryo!

A great pet peeve of mine is when clothing companies refer to plastic garments as "vegan leather." Throwing a virtuous health halo around a petroleum product is wild. Next it'll be all-natural organic cyanide

A long read from 2017 but well worth the time for any scientist who both loves their job and and often finds themselves questioning their life decisions. www.danielnettle.org.uk/wp-content/u...

A microRNA is the effector gene of a classic evolutionary hotspot locus In Lepidoptera (butterflies and moths), the genomic region around the gene cortex is a “hotspot” locus, repeatedly implicated in generating intraspecific melanic wing color polymorphisms across 100 mi...

Our miRNA story is now in @science.org ! We found a microRNA, not a protein, that finally solved a long-standing evolutionary mystery of wing coloration in butterflies and moths. (1/n)
www.science.org/doi/10.1126/...

🚨 PhD opportunity 🚨 The Whelan lab, along w @brockhurstlab.bsky.social, are looking for a PhD student w bioinformatic experience/interest to investigate the evolution of transmissibility in bacterial pathogens, with a focus on P. aeruginosa. More info & link to apply below.

Please spread the word!