Finally, I can be proud of my country!
❤️🤍💚

Before this day ends!

Have spent a day and a half on something that will save me around 2 hours every month. Worth it. xkcd.com/1205/

Under Review with Dr Elisabeth Bik - Research fraud detection, papermills and that rat Spotify video

In this episode of the Under Review podcast, I talk to @andytattersall.bsky.social about research fraud detection, papermills, and that rat.

open.spotify.com/episode/0pYD...

Beautiful ☀️y day, finally.

Standardized measurements for monitoring and comparing multiphoton microscope systems - Nature Protocols A set of procedures is described here for standardizing the evaluation of multiphoton microscope performance, covering laser power, pulse width optimizations, field of view, resolution and photomultip...

Fortunately, there's a resource to improve benchmarking of multiphoton systems. So things can get better. Here's the paper www.nature.com/articles/s41... And here's the free PMC version PDF (pmc.ncbi.nlm.nih.gov/articles/PMC...) (3/3)

Comparing multiphoton (2p/3p) imaging systems is often done poorly. labrigger.com/blog/2026/01... E.g., PSFs can be measured differently by different labs, staining quality is by far the biggest determinate on how multiphoton images will look at depth, and so forth. (1/3)

Spectacular views today!

We spend way too much time manually analyzing #microtubules 🔬

Together with Mario, Justus, Dominik, Felix & Erik we used synthetic data and foundation models to build a fully automated segmentation pipeline.

#microscopy, #imageanalysis & #AI

Check out our preprint:
www.biorxiv.org/content/10.6...

Building blocks! 🙂

The images shows an array of 6 Expansion microscopy images of cell nuclei (three columns and 2 rows). each image was taken with a different SPY-DNA probe to stain the nuclear DNA and is pseudocolored to the fluorescence wavelength of the SPY-DNA probe. Trom top left to bottom right: SPY505-DNA (green); SPY555-DNA (magenta); SPY595-DNA (yellow); SPY620-DNA (orange); SPY650-DNA (red); SPY700-DNA (darker red). a white scale bar of 50 micrometers is given for each image to get an impression of the scale of each nuclei.

Are you staining DNA in your ExM samples?

We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.

You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.

spirochrome.com/products/?_c...

Believe me when I say that I'm overlooking Barcelona. 🙂

The compulsory Christmas day ride! ✅ 🙂🎄🌟

Wuhuuu, preprints using the #RxivMaker engine are popping 🍾

Our annual PhD call is closing at the end of this week on 30 November. If you're interested in carrying out world-class scientific research in Barcelona, you still have a few days left to submit your application! www.crg.eu/en/content/t...

We just released a new major version of TrackMate (v8), the cell and organelle tracking plugin of Fiji.

It ships many new features, detailed below, but that are articulated around the following:

My two favorite CUBEs 🙂

In science a misconduct is a misconduct, whether accidental or deliberate. Ignorance is not an excuse.

FPbase Fluorescence Spectra Viewer An interactive fluorescence spectra viewer to evaluate the spectral properties of fluorescent proteins, organic dyes, filters, and detectors.

The FPbase spectra viewer just had it's biggest software refactor in 6 years. It *should* be largely invisible to the end user (hopefully just faster and less buggy). Please let me know if you find any issues as you use it!

www.fpbase.org/spectra/

I have two open positions @ALMU @crg.eu:

1. Imaging Scientist (permanent position. Deadline 11th Nov.) recruitment.crg.eu/content/jobs...

2. Entry-Level Imaging Scientist (12 months fixed-term position. Deadline 18th Nov.) recruitment.crg.eu/content/jobs...

Reposts appreciated.

Interested in intracellular organization and membrane trafficking? 🥞 Join us at @melisupf.bsky.social in Barcelona! We’re looking for postdocs to apply for a Juan de la Cierva fellowship (PhD between Jan’24–Dec ’25). If you’d like to work with us, get in touch! www.aei.gob.es/en/announcem...

Diada Montserratina ✅
In memoriam duorum patrum.

I did not know that the info I was looking for is right next to the canteen, just needed to walk the other direction. In exploring mode...

Thanks for sharing! 🙂

Episode 17 - perhaps my favorite yet. How do you write a forum.image.sc post that will get you the answers you need without revealing information you don't want to tell? @erinweisbart.bsky.social and I go through what the experts need to know to help you. Post your sci q's today on forum.image.sc !

Are you looking for a PhD? Join us in Barcelona! You'll dive into a community of >100 PhD students from 30 countries exploring the frontiers of biology. You can also join an online workshop on 6 November (15:00 CET) to learn how to find the right lab for you.

More info: www.crg.eu/en/content/t...

"The world has changed.
...
Much that once was is lost,
For none now live who remember it.”
― J.R.R. Tolkien

Cerdanyola del Vallès

🔬 Community Workshop #1: "Intro to Instrumentation QC in Light Microscopy"
🔹 Learn to monitor laser power
🔹 Record & inspect PSFs
🔹 Analyze data using FIJI + MetroloJ_QC

👩‍🔬Facilitators from @gimmfoundation.bsky.social, @crg.eu & Nova Medical School (NMS).

Let’s build better #microscopy!

#SPAOM2025

The inside of an SP8! Bringing it's 633 nm laser back to life from SP5 parts!

Once is an exception, twice is a trend, thrice is a tradition!

Save the date for our now-traditional annual OME-NGFF extravaganza in Zurich!

Whether you’ve just heard about OME-NGFF and are curious to learn more or if you are working with it every day, there is something for everyone this year!