What if you could cut out sub cellular structures and measure the proteome? SPEx: Compartment-Resolved Proteomics via Expansion Microscopy–Guided Microdissection
www.biorxiv.org/content/10.6...
awesome work by Curdin Franziscus, @imcf-lex.bsky.social,
@biehli.bsky.social, Alexander Schmidt!

A surface rendered view of an endosome. The endosome surface is rendered in blue with dimples on it. In specific regions of the endosomal surface, clusters of proteins are seen as aggregates of blobs, coloured in yellow and red.

🚨New paper out today in @acs.org ACS Nano, showing how expansion microscopy can visually quantify the cell surface receptor turnover mediated by signalling & receptor organising machineries on the surfaces of endosomes, some of the smallest organelles in cells. 🧪 🔬 1/ pubs.acs.org/doi/10.1021/...

How is expansion microscopy pushing beyond the original ~4× expansion limit, to unlock new ways to visualise biological structure at increasingly fine detail? Check out our review article "𝐁𝐞𝐲𝐨𝐧𝐝 𝟰×: 𝐩𝐚𝐭𝐡𝐰𝐚𝐲𝐬 𝐭𝐨 𝐡𝐢𝐠𝐡𝐞𝐫 𝐞𝐱𝐩𝐚𝐧𝐬𝐢𝐨𝐧 𝐟𝐚𝐜𝐭𝐨𝐫𝐬 𝐢𝐧 𝐞𝐱𝐩𝐚𝐧𝐬𝐢𝐨𝐧 𝐦𝐢𝐜𝐫𝐨𝐬𝐜𝐨𝐩𝐲" www.degruyterbrill.com/document/doi...

Expansion Microscopy Has Transformed How We See the Cellular World | Quanta Magazine How physically magnifying objects using a key ingredient in diapers has opened an unprecedented view of the microbial world.

Even with the largest zoom on a classic compound optical system, scientists struggle to make sense of finer details. Microbiologists are turning to an unexpected source to clear things up: a moisture-absorbing material found in diapers. www.quantamagazine.org/expansion-mi...

Comparison of the cytoskeletal architectures of Toxoplasma and Chromera flagellates using ExM.

Isadonna Tengganu and Ke Hu develop transfection and ExM protocols for Chromera velia, enabling direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondi.
journals.biologists.com/jcs/article/...

But none of our existing imaging methods (SoRa, STORM…) could really visualize integration within the bundled microtubules in neurites. So Ciarán set up U-ExM expansion microscopy, then performed it on microinjected neurons - now we could see plus-end growth and in-lattice renewal everywhere! 🧵 7/9

Introducing Cyclically Multiplexed Expansion Microscopy (Cy-ExM): a workflow for 3D nanoscale, high-plex imaging in whole cells. Cryo-preserved ultrastructure + iterative labeling + expansion microscopy → 20 targets in one dataset with ~70 nm lateral resolution.

www.biorxiv.org/content/10.6...

Last chance to apply for 'Ultrastructure expansion microscopy: from cells to tissue' yet? #EMBOExM

🔬 Advance your microscopy skills by joining us to explore U-ExM techniques and gain hands-on expertise in applying this powerful approach to mammalian cells.

Apply now: s.embl.org/uex26-01-bl

The images shows an array of 6 Expansion microscopy images of cell nuclei (three columns and 2 rows). each image was taken with a different SPY-DNA probe to stain the nuclear DNA and is pseudocolored to the fluorescence wavelength of the SPY-DNA probe. Trom top left to bottom right: SPY505-DNA (green); SPY555-DNA (magenta); SPY595-DNA (yellow); SPY620-DNA (orange); SPY650-DNA (red); SPY700-DNA (darker red). a white scale bar of 50 micrometers is given for each image to get an impression of the scale of each nuclei.

Are you staining DNA in your ExM samples?

We have awesome news for you! All our 6 SPY-DNA probes work beautifully well with the U-ExM protocol.

You can now tune the color of the DNA channel to your needs, according to the fluorophores on your preferred antibodies.

spirochrome.com/products/?_c...