Microparticle-enabled single cell multiparameter electronic immunophenotyping for selective electroporation Electroporation (EP) is one of the leading non-viral intracellular delivery methods used in various applications across research and cell therapy development and manufacturing. Currently widely used b...

Next chapter of our selective/targeted single cell electroporation mediated gene delivery story is out now👇led by Madeline Hoyle & co-authors @labonachip.rsc.org @gtresearch.bsky.social
pubs.rsc.org/en/content/a...

Electronic Detection of Functional Cellular Immunity Using Enzymatic Metallization Accurate detection of immune cell function at the single-cell level is needed for diagnostic and research applications, yet conventional methods such as flow cytometry require complex instrumentation and fluorescent labeling which limits their use in resource-poor settings. To address this limitation, we developed Electronic Phenotyping using Impedance Cytometry (EPIC), a platform that combines antibody-directed enzymatic metallization on the cell surface with multifrequency impedance analysis, in a microscale 3D-printed plastic aperture, to electronically detect surface marker-based metallization and cytokine secretion. Using CD45-targeted metallization, EPIC generated distinct impedance signatures in Jurkat cells and primary human peripheral blood mononuclear cells, with impedance changes correlating to surface metallization. A bispecific capture strategy coupled to the metallization was then used for detection of IFN-γ secretion of cells, with impedance readouts matching flow cytometry. These findings demonstrate the ability of the EPIC system for sensitive electronic immune profiling and support its potential as a scalable, low-cost alternative to fluorescence-based assays for point-of-care cellular immunity based diagnostics.

Electronic Detection of Functional Cellular Immunity Using Enzymatic Metallization | ACS Omega @gatechengineers.bsky.social @gtresearch.bsky.social pubs.acs.org/doi/10.1021/...

Cover art showing metallized cell

Desert rose

New cover art for a new paper. One thing this particular chemistry does very reliably - beyond making electronic detection of all kinds of things possible - is generating pretty images under the electron microscope. 😀That's a cell btw - not a desert rose crystal. Paper linked 👇 @pubs.acs.org

Led by Madeline Hoyle (1st 1st author paper for her - yay!) & co-authored with Julio Arroyo and electroporation guru Rafael Davalos @gatechengineers.bsky.social @gtresearch.bsky.social

A critical review of microfluidic electroporation for therapeutic cell engineering Microfluidic electroporation (MFEP) is an emerging, scalable, non-viral approach for engineering therapeutic cells, enabling high-throughput continuous processing, single-cell manipulation, reduced el...

Microfluidic electroporation has existed as a virus-free delivery alternative for decades now (since pre-Jesse Gelsinger gene therapy era almost) & yet even in cell therapy era why no translational use yet? We review (& opine 😉) here. 1st review article from the lab. www.cell.com/cell-reports...

New preprint from our lab 👇

Microscale Multiplexed Antigen-Specific Antibody Fc Profiling for Point-of-Care Diagnosis of Tuberculosis Accurate, affordable tuberculosis (TB) diagnostics that do not require sputum samples are urgently needed for TB control and elimination. Prior serological TB tests have failed due to low accuracy in ...

Freshly uploaded preprint: A new approach to TB diagnostics - from a drop of blood instead of sputum.
www.medrxiv.org/content/10.1...

Happens in 5mins! I know right after such a short lunch break is a tough slot so just reminding folks 👇

Thu Oct 9 1.15pm Room 26A

Friday Oct 10 5.15pm Room 26A

If you're in sunny San Diego for #BMES2025 do check out the talks from our lab on Thu & Fri👇

Thank you @labonachip.rsc.org for highlighting our work!

NOA

New NOA (phew!😀): MPI R21 with Dr. Lisa Marie Cranmer at Emory to use our antibody-omics platform for TB in children affected by HIV. Grateful for the support to continue and extend this important work. @niaidnews.bsky.social @gtresearchnews.bsky.social

New paper from the lab👇Congrats to Spencer Zhang
(@gatechengineers.bsky.social PhD student) and co-authors. Here we port our antibody-omics workflow to a easy-to-make (i.e. lithography-free) and easy-to-use (i.e. gravity/capillary flow driven) microfluidic chip. pubs.rsc.org/en/content/a...

PhD Hooding at Georgia Tech

PhD Hooding at Georgia Tech

Passing the baton - or the PhD hood in this case - to the first PhD from our lab @gatechengineers.bsky.social. Congratulations to the newly minted Dr Rudge! Reminded me of a rainy summer day many years back @mit.edu but thankfully weather gods here relented after just a slight shower yesterday.

Happening later this afternoon 👇. I know this venue has felt like a maze to me but we'll be in Hall I just past the poster hall (which is also Hall I but not the one we're in 😀) & just before the badge pickup counters.

Abstract 8267: Schisto morbidity biomarker

Abstract 7472: Schisto/TB crosstalk

Abstract 6575: Leprosy biomarker

Abstract 6081: HIV/TB co-infection biomarker

Excited to be at #TropMed24 in beautiful New Orleans! If you're here, do come see us at our presentations on antibody-omics in various diseases: Sarah Ali's talk on HIV/TB co-infection (today @ 10.15!) & Pedro Marcal's 3 posters on Leprosy, Schistosomiasis & Schisto/TB crosstalk.

If you're at ASTMH #TropMed24 in New Orleans do add our Systems Immunology of Tropical Diseases: Harnessing Omics & AI for Global Health symposium (138) to your schedule for Sat Nov 16th. Exciting talks across AI/ML, Malaria, TB & NTDs including Schistosomiasis.

High throughput electronic detection of biomarkers using enzymatically amplified metallization on nanostructured surfaces Enzyme-linked immunosorbent assays are commonly used for clinical biomarker detection. However, they remain resource-intensive and difficult to scale globally. Here we present a miniaturized direct el...

New paper from our lab: A high-throughput microchip & a handheld 'plate reader' for direct electronic biomarker detection. Led by Spencer Zhang & Neda Rafat.
pubs.rsc.org/en/content/a...