The first ever cover art from my lab! And an awesome story about how chromatin changes in response to the N/C ratio from my first grad student, Anusha Bhatt!

Just a few days left to apply! If you do cool molecular/cell biology things and want to start a lab in a supportive department in New England please check out our ad.

My Department (Biology) at @dartmouthartsci.bsky.social is hiring this year! We have an opening for a Tenure Track Cell and/or Molecular biologist (broadly defined).

Please share with your trainees/labmates/friends and reach out if you have questions.

apply.interfolio.com/171625

FlyBase needs your help! We ask that European labs continue to contribute to Cambridge, UK FlyBase, whereas US and other non-European labs can contribute to US FlyBase. For more information and how to donate: wiki.flybase.org/wiki/FlyBase...

Please reach out if you/your trainees have any questions. And please apply!

As someone who started here in 2020, I feel lucky that we will be able to hire this cycle since I know how nerve-wracking the faculty job search can be, especially in years where external factors make the process even less predictable than usual.

At Dartmouth Biology we have a great graduate program in molecular and cell biology (@mcbdartmouth.bsky.social), a kind and supportive departmental culture, and solid core support all set in the bucolic Connecticut river valley in Hanover, NH.

My Department (Biology) at @dartmouthartsci.bsky.social is hiring this year! We have an opening for a Tenure Track Cell and/or Molecular biologist (broadly defined).

Please share with your trainees/labmates/friends and reach out if you have questions.

apply.interfolio.com/171625

Oh man, you guys. This is so important. Drosophila have led to so much in terms of what we understand about how genes work.

Photo of 5 fly biologists in funny costumes in front of the Dros25 banner.

Dartmouth at #Dros25

#Dros25 is off to a great start!

If you're interested in zygotic genome activation & chromatin my lab has 2 great folks here:

Anusha Bhatt has a talk on histone H3/H3.3 replacement in the chromatin session on Fri morning

Grace Carey has a poster on N/C ratio sensing in ZGA on Sat afternoon

U.S. Senate: Contacting U.S. Senators Please include your return postal mailing address when corresponding with a Senate office.

Stop the halt of NIH!
I am reaching out to ask that you call your US Senators/Reps. Especially those of you in red states! Tell them how important the NIH is!

www.senate.gov/senators/sen...

Society for Developmental Biology Logo - Choose Development Paid Summer Undergraduate Research Training Mission To broaden the scientific workforce by preparing undergraduate students to enter doctoral programs in developmental biology with enriched mentored research experiences for two summers. Overview Two 10-week summer research internships in the lab of an SDB member Receive $5500 stipend each summer Receive guidance and support from a team of mentors Network with the developmental biology community Present summer research at the next SDB annual meeting Eligibility Undergraduate student from underrepresented group(s), first generation college student, veteran, or undergraduate student with a disability U.S. citizen or permanent resident Interest to pursue a doctoral degree Photo of woman in lab coat pipetting a solution QR Code to Choose Development website Application Deadline: February 1, 2025 www.sdbonline.org/choose_development

There are two weeks left to apply for the #ChooseDevelopment! Summer Undergraduate Research Program. Undergrads from underrepresented racial or ethnic group, first-generation college students, veterans & students with a disability are eligible. bit.ly/3FwHqYp Deadline: February 1, 2025 #devbio

Research Assistant I To set up and conduct laboratory tests and experiments in support of Dr. Amodeo’s research studies by applying established methods, procedures, and techniques in genetics, biochemistry, and quantitati...

Don't hesitate to contact me with any questions.

Apply here: searchjobs.dartmouth.edu/postings/772...

Lab website: sites.dartmouth.edu/amodeolab/

My lab is looking to hire a research technician!

Techs in my group run their own projects and participate fully in the scientific discussions in the lab (this is not a wash glassware, make media position). Ideal for someone looking to get a bit more research experience before applying for a PhD.

Link to core director position:
apply.interfolio.com/154316

Link to Protein Biochemist – Research Scientist position:
apply.interfolio.com/161499

Our Institute for Biomolecular Targeting (bioMT) is looking for a *new core director* and protein purification specialist. If you're interested in working on a wide array of projects and living in the peaceful Upper Valley this is an excellent place to work. Links below.

sites.dartmouth.edu/biomt/

These results surprised us and have us thinking about zygotic chromatin composition in totally new ways! As always, biology is way more interesting and complicated than out overly simplistic initial explanations.

Finally we used Chk1 to prevent cell cycle slowing and that did decrease H3.3 chromatin incorporation. BUT, it wasn't due to the cell cycle since H3.3 decrease precedes the change in cell cycle length!

Next we thought that maybe H3.3 incorporation was downstream of transcription, but when she knocked down Zelda H3.3 was still incorporated just fine.

In the first version of this paper we proposed that this was due to exhaustion of the (prefered) maternal H3 supply and that H3.3 was incorporated as a "second choice" H3. But when Anusha actually reduced the level of H3... H3.3 incorporation was unaffected!

We made made H3/H3.3 chimeras and saw that chaperone binding sites were key. Also, H3/H3.3 chromatin levels depend on the local Nuclear to cytoplasmic ratio. But how do histones know the N/C ratio.

We started out trying to explain why H3 goes up and H3.3 goes down on chromatin leading up to ZGA in the fly embryo. This is interesting because H3.3 is associated with both active transcription and heterochromatin which appear at ZGA.

Local nuclear to cytoplasmic ratio regulates H3.3 incorporation via cell cycle state during zygotic genome activation Early embryos often have unique chromatin states prior to zygotic genome activation (ZGA). In Drosophila, ZGA occurs after 13 reductive nuclear divisions during which the nuclear to cytoplasmic (N/C) ...

First post on bsky. We've updated our preprint about the change in the histone H3 variant on chromatin during Zygotic Genome Activation. Thanks to new experiments by Anusha, it seems like our initial, simplistic idea that the embryo "ran out" of H3 was not right.

www.biorxiv.org/content/10.1...