Please also check out another exciting work on AI-based protein design, which was a fantastic team effort with Michael Hölzel, @gha14.bsky.social, Tobias Bald and many colleagues at @unibonn.bsky.social
www.biorxiv.org/content/10.1...
We are grateful to the Hans und Ria Messer Stiftung and the cluster of excellence @immunosens.bsky.social for supporting our research at @unibonn.bsky.social and Uniklinikum Bonn
This work was led by the phenomenal team of Alexander Hoch, Caroline Fandrey, Thomas Ebert in collaboration with Brian Coventry and David Baker at @uwproteindesign.bsky.social, Tim Kempchen at the Hölzel lab, and Sebastian Virreira Winter and Philipp Geyer from ions.bio
Not yet in the preprint: Mass spectrometry confirmed the highly specific degradation of one target protein out of 9,677 human proteins that were quantified in cell lysates
Fusing AI-binders to a mutant FKBP12 domain enabled drug-controlled degradation of the endogenous target protein in human macrophages
Coupling AI-binders to EGFP enabled the antibody-free staining of an endogenous protein in primary human macrophages
Using Transposon-Display, we identified binders for four intracellular human proteins and further improved them by saturation mutagenesis
We developed a technology called Transposon-Display, which couples AI binders to their own DNA for pooled screening