It is an opportunity to discuss these methods with those experienced in this field and to develop a peer network for those embarking spatial proteomics programs.

It will provide an opportunity for scientists interested in applying this tool to tailor an approach their protist(s) systems of interest, or those who are simply curious.

This provides a huge window of opportunity in protistology that is being embraced by many labs. This workshop is aimed at a broad protistology audience to present the ways in which spatial proteomes can be generated and interpreted.

But it has presented the challenge of interpreting this molecular diversity and, in particular, vast cellular novelty. Spatial proteomics methods (aka 'LOPIT’) enables thousands of proteins to be simultaneously assigned subcellular locations (organelles, complexes, substructures and symbionts).

The genomics field has driven much of ‘modern’ protistology's advances in recent years providing a phylogenetic frameworks for understanding protist evolution, and an appreciation of the molecular novelty of this wide snapshot of eukaryotic diversity.

Spatial Proteomics Workshop for Protists

(see more in thread below)

Registration
Early Bird Monday 22nd June 2026.
Regular Monday 10th August 2026.
There will be a conference dinner on day 1. To register visit protistology.org.uk/autumn-meeti...

This has been a great collaboration with Julian Rayner and Kathryn Lilley’s groups. A pleasure working with this team: Scott A. Chisholm, Victor Flores, Alison Kemp, Lisa M. Breckels, Ludek Koreny, Nicolas Dos Santos Pacheco, Konstantin Barylyuk, Anna Kuroshchenkova.
@nicolas3474.bsky.social

These data can be interactively explored with two ShinyApps (thanks Lisa Breckels)
proteome.shinyapps.io/plasmolopitsz/
proteome.shinyapps.io/plasmolopits...
because there is much more evidence of protein association and interaction awaiting discovery here.

These data provide a view of how Plasmodium parasites have, and continue to, evolve their compartments through protein gains, losses, and adaptations.
@vflorelo.bsky.social

Excited to release the Herculean efforts of @scottchisholm.bsky.social &Co. defining the subcellular #hyperLOPIT spatial proteome of #Plasmodium schizonts. Proteomes defining 24 subcellular niches, including exported compartments in the blood cell, are identified.
www.biorxiv.org/content/10.1...

Bath city centre

Pulteney weir in Bath

Bath city centre

Bath cathedral

🎉 There are currently 4 opportunities to join the Holt lab in beautiful Bath! 🎉

If you're interested in protists, parasites, and/or biodiversity then please check out our website:
theholtlab.com/posts/ln5-21...

🚨 Deadlines vary between project.
✉️ Please reach out if you have any questions!

I’m very excited to be working with this great team. If the kickoff meeting in Bern was any measure, it’s going to be a brilliant project.