An accessible highlight of our recent article:
How Single-Cell Proteomics Could Unlock the Rules That Govern Cells
azolifesciences.com/news/2026041...
Article:
Single-Cell Proteomic Technologies: Tools in the Quest for Principles. www.annualreviews.org/content/jour...
Judging from the abstracts submitted to the single-cell proteomics conference, SCP2026 is shaping to be an exceptional meeting.
A convergence of new technologies, deep questions, and inventive experimental designs is poised to unlock answers to long standing puzzles.
Abstract deadline: May 1st
More details in this review article: Single-Cell Proteomic Technologies: Tools in the Quest for Principles
www.annualreviews.org/content/jour...
Open access link: slavovlab.net/Slavov-Lab-P...
Single-cell proteomics by mass spectrometry: Technological overview and recent progress
From uncertain possibility to robust and rapidly advancing technologies:
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youtu.be/tibkXV6NblI?...
A corner stone of cell models:
mRNA abundance scales with cell size & growth rate.
Changes in growth rate shift thousands of mRNA levels coordinately.
Thus, the transcriptional responses to perturbations often reflect the associated growth rate changes.
www.molbiolcell.org/doi/full/10....
A powerful example of protein abundance regulation.
The degradation rate of p53 determines its abundance.
p53 is special, but regulation by protein degradation is not a special case. It's common:
doi.org/10.1101/2025...
This July 14 - 15, the 9th Single-Cell Proteomics Conference (single-cell.net) brings together a community that is redefining whatโs possible: Robust methods enable scalable proteoform measurements from single cells without sacrificing depth or quantitative accuracy.
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Six years on from this perspective: science.org/doi/10.1126/...
The technology has rapidly advanced and spread beyond a few pioneering labs.
I am excited for the next phase, when the technology will increasingly enable mechanistic investigations & discoveries.
๐
Here is a robust biochemical & functional difference between young and old mammalian cells:
- The fraction of new amino acids incorporated into proteins.
The difference is consistent, quantitative, cell-type specific, and deeply intriguing.
What aging mechanisms cause it ?
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At the same time, some proteins are very long-lived. Some persist as long as the organism itself.
Protein lifetimes range from minutes to decades:
that's a dynamic range of ~6,000,000-fold.
Lifetimes vary across cell types & states, and these dynamics are essential to life!
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hashFrag aims to partition data with minimal leakage and thus improve estimates of model performance.
www.biorxiv.org/content/10.1...
A recurrent theme in ML:
โผ๏ธ Poorly trained models appear to excel at predictions.
"Because the sequence and its function are inexorably linked, even a maximally overfit model with no understanding of gene regulation can predict the expression of sequences that are similar to its training data."
Molecular features of human pathological tau distinguish tauopathy-associated dementias
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www.cell.com/cell/fulltex...
Post-translational protein modifications underpin protein functions and dysfunctions.
=> Naturally, proteoforms enable molecular disease stratification.
This resource article maps 145 PTMs and 195 cleavage sites on tau and uses them as biomarkers for tauopathies.
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Iโm grateful to be named a COE Distinguished Professor by Northeastern University.
This recognition reflects the support, collaboration, and creativity of many students, and colleagues over the years. Iโm thankful to be part of a community that values rigorous science, and mentorship.
Discussing research with the innovators beats scrolling a feed.
If youโre in single-cell biology, proteomics, mass spec tech, or computational biology, join our next meeting this July.
#SCP2026
Single-cell proteomics is moving past descriptive snapshots to quantitative models of cellular regulation and biological mechanisms.
Come to #Boston this July to shape the next chapter and have your voice heard.
Abstract deadline: Apr 1, 2026.
single-cell.net/proteomics/s...
Protein degradation is often considered a final step in gene expression regulation, but it also regulates the first step: Transcription
Many TFs, including p53, HIF-1ฮฑ and c-Myc, are regulated by ๐๐๐ ๐ซ๐๐๐๐ญ๐ข๐จ๐ง-๐ ๐๐ญ๐๐ ๐๐จ๐ง๐ญ๐ซ๐จ๐ฅ:
โฌ๏ธ It has a key advantage.
blog.slavovlab.net/2025/08/16/p...
Transformative discoveries, such as restriction enzymes, yeast cell-cycle mutants and CRISPRโCas9, were once considered simply oddities of nature.
Letโs adopt more humility about predicting impact, which can truly be known only in retrospect.
Happy and successful 2026 !
It will be a productive one for single-cell proteomics.
single-cell.net/proteomics/s...
We should create a culture that encourages and stimulates creativity, mentoring and supporting the growth of young researchers towards creative intellectuals.
Links to the code, data and more supporting information are available at the supporting website:
scp.slavovlab.net/Iwamoto-Stoh...
Our latest article is out in Cell !
Together with the Zernicka-Goetz lab, we show that fertilization triggers proteomic asymmetry in mammalian zygotes, giving rise to alpha vs. beta 2-cell blastomeres with distinct developmental potential.
cell.com/cell/fulltex...
The interview covers the trajectory from early, high-risk ideas to robust, scalable workflows.
Much has changed, and progress continues unabated.
๐๐ฏ๐๐๐ฟ๐ฎ๐ฐ๐ ๐๐๐ฏ๐บ๐ถ๐๐๐ถ๐ผ๐ป ๐ถ๐ ๐ผ๐ฝ๐ฒ๐ป !
The 9th Single Cell Proteomics Conference (#SCP2026) will take place in #Boston, July 2026.
The Single Cell Proteomics Conference focuses on providing opportunities for scientific discussions, interactions and community development.
single-cell.net/proteomics/s...
This feature highlights early advances from our group in liquid-chromatography mass spectrometry (LC-MS) for single-cell proteomics.
center.single-cell.net/single-cell-...
he multiplexing capabilities introduced by Parallel Squared Technology Institute (@parallelsq.bsky.social) can substantially increase the scalability of these methods.
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www.parallelsq.org/publications
We are exited about the experimental opportunities enabled by these approaches and the potential to increasingly bring dynamics to omics scale analyses of tissues at single-cell resolution.
Mass spectrometry enables omics scale quantification of the rates of macromolecule synthesis and degradation in the single cells of a living mammalian tissue.
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The majority of isoform pairs (of alternatively spliced transcripts) share less than 50% of their interactions.
In the context of interactome networks, alternative isoforms tend to behave like distinct proteins rather than minor variants of each other.