SPEx: Compartment-Resolved Proteomics via Expansion Microscopy-Guided Microdissection www.biorxiv.org/content/10.64898/2026.03...

Cell-type resolved protein atlas of brain lysosomes identifies SLC45A1-associated disease as a lysosomal disorder This study describes the cell-type-specific lysosomal proteome across the major brain cell types and identifies SLC45A1 as a neuron-specific lysosomal sugar exporter linked to lysosomal storage disease.

Now online! Cell-type resolved protein atlas of brain lysosomes identifies SLC45A1-associated disease as a lysosomal disorder

Membrane editing with proximity labeling reveals regulators of lipid homeostasis - Nature Chemical Biology Coupling an optogenetic lipid-modifying enzyme with proximity labeling reveals protein networks and mechanisms regulating lipid homeostasis in the membranes of target organelles.

Thrilled to share our latest study, led by @reikatei.bsky.social, in @natchembio.nature.com! We began by asking a simple question—how do cells know if they have too much of a lipid in a particular membrane, and how do they respond to rectify this imbalance?
www.nature.com/articles/s41...
More info 👇

A super detailed protocol + video on Cryo-ExM - Cryo-Expansion Microscopy, led by the labs of our former postdocs @marinelap.bsky.social & @ebertiaux.bsky.social.
Clear, practical, and very useful for anyone doing nanoscale imaging 🚀 app.jove.com/t/68595/expa...

Latest preprint from our lab reports that the distinct pH of anterograde (less acidic) and retrograde (more acidic) lysosomal vesicles in the axon depends on assembly of the V1 and V0 domains of the vacuolar H+ ATPase, mediated by the metazoan RAVE complex www.biorxiv.org/content/10.6...

🚀 Our new paper is out @natmethods.nature.com!

Kuffer & Marzilli engineered conditionally stable MS2 & PP7 coat proteins (dMCP & dPCP) that degrade unless bound to RNA, enabling ultra–low-background, single-mRNA imaging in live cells.

🔗 www.nature.com/articles/s41...
🧬 www.addgene.org/John_Ngo/

The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development Proper neuronal development and function rely on the polarized sorting of transmembrane proteins (TMPs) to their distinct somatodendritic or axonal domains. Most TMPs follow a conventional secretory p...

The axonal ER couples translation and secretion machineries for local delivery of axonal transmembrane proteins to promote axonal development www.biorxiv.org/content/10.1...

Wow, this paper provides crucial understanding of local translation and processing of transmembrane proteins in axons, one of the big mysteries that have haunted the field for a long time!!!!!!!! Fantastic!!!!!!!! Nguyen et al., 2025, bioRxiv 2025.09.09.674816ff. -- doi.org/10.1101/2025...

Excited to share our preprint, now available online on BioRxiv! biorxiv.org/content/10.1.... We uncovered the coupling of translation and secretion machineries at the #axonalER to promote local delivery of axonal transmembrane proteins (TMPs) to the plasma membrane.

The involvement of the ER-phagy receptor FAM134B in membrane contact sites between ER and endolysosomes promotes ERLAD www.biorxiv.org/content/10.1101/2025.07....

Multi-organelle-mediated mRNA localization in neurons and links to disease Brain function requires precise spatiotemporal regulation of the neuronal proteome. To allow adaptation of the proteome in distal outposts of neurons,…

The first review paper from our lab is out!! @viviennebauer.bsky.social discussed the rapidly emerging knowledge on (the interplay between multiple) organelles and neuronal mRNA localization. Hope you enjoy the read! www.sciencedirect.com/science/arti...

Constructs are available on @addgene.bsky.social. Analysed data are available as Source data. Raw proteomics data are available on PRIDE.

Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neurons - Nature Communications It is unclear how new lysosomal proteins are properly delivered in polarized neurons. Here, the authors developed a method combining the synchronization of protein release from the endoplasmic reticul...

www.nature.com/articles/s41...

POTATOMap is officially out today! (Even tho it says 2024Dec30… yeah long story😂)

Thank you to all the authors and everyone involved! I hope we have provided something useful and maybe leave a little (just a little) impact in your research.