Dankjewel!

Thank you Emilie!!

Thirteen early-career researchers set to cross borders thanks to Rubicon grants | NWO No fewer than thirteen talented researchers who have recently completed their PhDs are being given the opportunity to gain valuable research experience abroad. The Rubicon round, organised by NWO, had...

I am very happy to have been awarded a Rubicon Postdoctoral fellowship from the NWO (Dutch Science Council) (Science/Life Sciences domain) for my postdoc in the @tothovalab.bsky.social at @danafarber.bsky.social / @broadinstitute.org!
www.nwo.nl/en/news/thir...

🧵 CTCF is essential for embryonic development, but why has remained unclear. By combining gastruloids with a temporal degron system, we uncovered a surprising dual function — and it changes how we think about CTCF's role in development. 1/8 www.biorxiv.org/content/10.6...

wow! thanks for sharing! this looks so useful!

Yes!! In september. Im now in the lab of zuzana tothova. I know... it was a revelation such a community existed though

@timtriche.bsky.social @paralkarlab.bsky.social the blood-themed valentine cards remind me a little of our initial struggles with trying to get out of the vampire vibes VS hematology community by using bloodsky instead of hemesky... Haha

having a look at my HemeSky feed for the first time in a while (have been on an off bluesky a bit) but this looks so interesting!
my HemeSky feed picks up interesting papers as well podcasts such as this one, and also blood-themed valentine cards 👀 (@ash.hematology.org ) :
bsky.app/profile/dpas...

Woww 🤯🤯 mind blown on saturday morning!! Must-read thread ⬇️

Haha yes it is - we were concerned about this being an issue but we had beautiful signal even though we used the blue laser. It smells a bit different as well 🤔 more floral or sweeter than dreft I think

Preprint alert: Jiangyuan Liu developed a new workflow for chromatin loop calling across Hi-C datasets, e.g., during differentiation. Most loops are shared between datasets/cell states. Important work for all interested in chromatin loops and how to identify them!

www.biorxiv.org/content/10.6...

Rotation PhD student @emiliedevet.bsky.social in our lab made the Dish soap protocol work (on the first attempt) for a nuclear target with Dawn (the US version of dreft/fairy ) and thereby extended the dish soap protocol to a version for across the atlantic 🤩 @tothovalab.bsky.social #hemesky

Progress and challenges in profiling protein-RNA and protein-associated RNA-RNA interactions - PubMed RNA binding proteins (RBPs) play essential roles in post-transcriptional gene regulation by interacting with a wide range of RNA targets. In addition to regulating RNA processing via individual RBP-RNA interactions, there is a growing appreciation of the regulatory impact of protein-associated RNA-R …

Happy to share Joey (Zhuoyi)'s review on technologies to map RBP:RNA and (especially) RBP-associated RNA:RNA interactions is now up at RNA! pubmed.ncbi.nlm.nih.gov/41535089

We are very intrigued - @emiliedevet.bsky.social is going to give Dawn a try this week! (The US version of fairy/dreft)

Thank you!

@bloodgenes.bsky.social I wanted to wait until the physical copy, sorry for my late reply haha

Thanks Vijay!! Congratulations to your manuscript too - really cool to see that it's out!

This was the first time I got to hold my own physical article this morning!! Very exciting

CEBPA repression by MECOM blocks differentiation to drive aggressive leukemias Key PointsMECOM promotes malignant stem cell–like states in aggressive AMLs by directly repressing prodifferentiation gene regulatory programs.A MECOM-boun

Thanks to all the co-authors - @delwelruud.bsky.social ! This paper was accompanied by a paper from @tjflemin.bsky.social and @bloodgenes.bsky.social with similar conclusions and very cool dCas9 experiments: ashpublications.org/blood/articl...

In this paper we show how dose-control of CEBPA is essential to maintain the phenotype of MECOM-rearranged AML. Notably, differentiation by MECOM degradation is able to switch of the enhancers driving MECOM expression over time. However, we don't know if differentiation alone will do the same.

In patient samples, CEBPA is reduced in patients that express MECOM through a 3q26-rearrangment or unknown mechanism of overexpression.

The upregulation of CEBPA is partially CTBP1/2 dependent, as overexpression of our earlier MECOM/CTBP interfering peptide PLDLS also increases CEBPA levels (but not the negative control PLASS).

Only upon MECOM depletion, the enhancer becomes truly active to upregulate CEBPA. This also leads to the appearance of a CEBPA footprint in ATAC-signal.

MECOM regulates CEBPA through binding to its +42kb enhancer; an enhancer that is bound by many TFs at baseline in our cells despite being largely inactive (in fact, it had been our positive control in ChIP-seq because of this).

We show that CEBPA is among the first genes to be upregulated upon acute MECOM loss.

Although this is a seemingly simple mechanism as noted in the commentary, it took a long time to find CEBPA as a target because of the huge effect MECOM on differentiation status. We solved this by using a degron model.

How do single transcription factors prevent lineage differentiation?
In this issue of Blood, the second paper of my PhD is published. We show that MECOM, a key HSC TF, maintains stem cell state by preventing change through repression of CEBPA. #hemesky ashpublications.org/blood/articl...

For our #flowcytometry peeps, would you like to have a single fix/perm protocol that is optimised for everything? One that preserves fluorophores while allowing simultaneous TF and cytokine staining? How about 100-fold cheaper?

You got it:
currentprotocols.onlinelibrary.wiley.com/doi/10.1002/...

Thanks cansu!!

Excited to share that I started as a Postdoctoral Fellow in @tothovalab.bsky.social at @danafarber.bsky.social in Boston! Looking forward to the next years of molecular & myeloid biology in Boston 🤩🌇