Love it! Can I e-mail you to ask for the vectors? I am enbarking on some endogenous tagging and this could be a very nice approach.

BLOC-1 and BORC help move and position lysosomes inside cells. New work from the Haselbach and Clausen labs shows they are not uniform complexes, but families of related protein assemblies. Published in PNAS: https://www.pnas.org/doi/10.1073/pnas.2515691123

I tried an even harder example on Gemini Pro image generation and this is quite scary/amazing. I asked for a microscopy image of around 20 HeLa cells, GFP tagged 20% nuclear, 10% membrane, +1 nuclear staining, + overlap. Image below and prompt in the following post.

Would such a probe work for other organelles, eg mitochondria or endosomes, if tagged properly? Or does it need to be a continual network like ER has?

Hlasovaci listek si tisknem sami, prijde pry jen identifikacni listek.

Sléz velkokvětý nebo neco tomu podobneho. Kakosty maji trochu jiny tvar kvetu. Jiná čeleď :)

Sléz,ne?

In our cover, Waich et al. record progress for early-onset O2HE Syndrome–their analysis of the UNC45A c.710T>C 37 missense variant uncovered its pathogenic mechanisms & recessive 46 characteristics and may aid in the development of targeted therapies: buff.ly/sU0qlNx

Practical recommendations for developing software for life science applications ABSTRACT. Developing user-friendly image analysis software is essential for advancing biological and life science research. However, the interdisciplinary gap between software developers and life scie...

Excellent set of guidelines for anyone developing software for life scientists, from @jwpylvanainen.bsky.social, @lankylaste.bsky.social and @guijacquemet.bsky.social

journals.biologists.com/jcs/article/...

Oh, I just add a few grams of RNase to every meal and let it incubate for a while.

An old timelapse I dug up... U937-derived macrophages bathed in green and red dextran... the green dextran is pH sensitive, leading to the appearance of mostly red lysosomes.

NEW: Russia Has Failed to Break Ukraine: isw.pub/RussiaRetros...

Russia dedicated staggering amounts of manpower and equipment to several major offensive efforts in Ukraine in 2024, intending to degrade Ukrainian defenses and seize the remainder of Donetsk and Luhansk oblasts. (1/3)

I might have not used exactly the same images for all 3 tools, sorry for that - I did this from two different laptops and picked slightly different images somewhere.

Difficult to generalize but Cellpose was probably simplest to install even though I ran the remaining two from Google Colab. Low-res images were easier to segment with all 3 tools (cell boundaries are clearly visible). MicroSam surprisingly good even with the confocal but still not reliable.

CellPose 3

MicroSam

SamCell results

For testing, I used these three DIC images (which I so far never segmented automatically and had to rely on manual ROI selection) - a tissue culture room microscope, an Incucyte image and a high-end confocal DIC channel

I actually tested these two and also SamCell (recently on Biorxiv). Here's the quick and dirty results - might still be finetuned, and I might have not always picked the best settings.

Mapy.cz: maps & navigation - Apps on Google Play Your guide to hiking, cycling, skiing and ski alps, by car and public transport

play.google.com/store/apps/d... Try this it's superior to Google maps in terms of map layout

Very nice! During our practical course we once used the ColorfulCell vector for a similar purpose www.addgene.org/62449/

Very nice! During our practical course we once used the ColorfulCell vector for a similar purpose www.addgene.org/62449/

Vimentin-mSG FRAP

You an even go as far as to color-code each cell-level datapoint by the experimental run it came from, so the reader can see if there if the cell variability is clustered or dispersed.

ER-lysosome contact sites (green) in MEFs on the background of an ER stain (grey, GFP-VAPA)