Thank you!!

Finally, I want to thank my mentor @mkrupovic.bsky.social , as well as @sgribaldo.bsky.social , Guillaume, and all the other colleagues from Institut Pasteur @institutpasteur.bsky.social for their support in this wonderful project!

Biogenesis of DNA-carrying extracellular vesicles by the dominant human gut methanogenic archaeon - Nature Communications Baquero et al. show that extracellular vesicles produced by the dominant human-gut archaeon are likely formed by budding from the cell membrane and released through disruptions in the cell wall, and c...

Here are the link to both studies:

Weinberger et al., Nat Comms: www.nature.com/articles/s41...

Baquero et al., Nat Comms: www.nature.com/articles/s41...

On the other hand, our colleagues (Weinberger et al., Nat Comms) used proteomics and metabolomics to study EVs from four human gut archaeal strains, revealing interesting cargos like adhesins, aspartic and glutamic acid, as well as notorious immune-stimulating properties.

Our Cryo-ET data also sheds light on the biogenesis of M. smithii EVs. The results suggest they form by budding from the cell membrane and get trapped under the peptidoglycan layer before being released through disruptions in the cell wall.

Characterization of M. smithii EVs

In our study, we show that EVs from Methanobrevibacter smithii — the predominant archaeon in the human gut — harbor proviral DNA and circular extrachromosomal DNA (eccDNA) that encodes key enzymes for methanogenesis, suggesting a potential impact on methane production in the gut.

Kicking off my account with great news🎉: our latest paper on extracellular vesicles from human gut archaea is out in @natcomms.nature.com with
@mkrupovic.bsky.social and @sgribaldo.bsky.social!
Published in parallel with the work of our colleagues from the Moissl-Eichinger Lab @chmoei.bsky.social🧵