Thanks KJ!

Our laboratory contributed to this work by resolving acidic patch-binding nanobodies developed by @epicypher.bsky.social. I’d like to thank our wonderful collaborators, and Emma Saccone (@emmasaccone.bsky.social)(data analysis, interprt., figs) and Heather Folkwein (@hjf3.bsky.social)(sample prep)

Figure showing nucleosomes bound by two different nanobodies. First row shows cryo-EM densities and models built based on them for each of two nanobodies. Second row informs about differential binding of each nanobody to nucleosomes. Third row describes contacts each nanobody makes with the surface of the nucleosome.

This work highlights a delicate balance between nucleosome acidic patch interactors critical for normal cellular functions and dysregulated in disease

A wonderful collaboration between Jess Tyler lab, @epicypher.bsky.social, @gcloner.bsky.social, James Kadonaga and our lab at PSU. In this article, we provide the significance of the nucleosome acidic patch. 

doi.org/10.64898/202...

Honored to be named the Laura and Isaac Perlmutter Endowed Chair in Biochemistry at NYU Grossman School of Medicine. Grateful to my mentors, lab members, and the NYU community—and most of all, to my amazing family for their constant support!

Thanks Aidan! Very on-point comment, I appreciate it - and you are right. Often, one tries to simplify the system by limiting the number of variables for a single study - but in reality it seems more like an elaborate albeit stochastic ballet dance :)

We coupled in depth mutagenesis and biochemistry with high-resolution cryoEM, obtaining maps ranging from 2.37 to 2.9 Å.

This work was done by Ilana Nodelman (Bowman lab) and Heather Folkwein (Armache lab)

Thank you to everyone involved. It was a fun joint-venture!

Thank you🙏

A competitive regulatory mechanism of the Chd1 remodeler is integral to distorting nucleosomal DNA - Nature Structural & Molecular Biology Nodelman, Folkwein et al. define a regulatory region in Chd1 containing adjacent inhibitor and activator elements that compete for binding to the remodeler ATPase. The competition between these elemen...

New collaborative paper between JPArmache and Bowman (@bowmanlab-jhu.bsky.social) labs show how the yeast CHD1 chromatin remodeler depends on activator elements to distort nucleosomal DNA. This explains how the NegC inhibitor blocks activity. www.nature.com/articles/s41...

This was a highly collaborative work between multiple labs, performed by a talented grad student in the Armache and Murakami labs, Natalie Smith. Thank you to all the authors for their incredible work!

We did a thing... We purified native transcriptional complexes from Drosophila embryos, obtaining +/- stalk Pol II elongation complexes, native nucleosomes, as well as a nucleosome elongation complex. For more details please check out: doi.org/10.1101/2025...

Read–write mechanisms of H2A ubiquitination by Polycomb repressive complex 1 Nature - Cryo-electron microscopy and biochemical studies elucidate the read–write mechanisms of non-canonical PRC1-containing RYBP in histone H2A lysine 119 monoubiquitination and their...

Please see our paper in Nature on read-write mechanisms of H2AK119 ubiquitination by Polycomb repressive complex I. Congrats to the whole team, especially Victoria and huge thanks to our collaborator JP Armache! Also big thanks to Mark Foundation for Cancer Research for the support! rdcu.be/dZ5HZ