It is all implemented as a Pyton-based open source control widget that seamlessly interfaces with a commercial @abberior.rocks MINFLUX microscope and its control software Imspector. Try it for yourself, and let me know if you have any questions! github.com/jonatanalvel...
etMINFLUX applications measuring 2D lipid diffusion at caveolae, and 3D membrane topography at dynamin-mediated endocytosis and virus budding sites.
etMINFLUX uses confocal monitoring and triggers MINFLUX acquisitions at rapid and rare events in living cells previously unreachable with MINFLUX, exemplified by tracking the 3D membrane topography in dynamin-mediated endocytosis (10 s) and virus budding (min) in action.
Excited to share my postdoc work from the Eggeling lab in Jena (@leibnizipht.bsky.social, #FSUJena, #KTHuniversity) on bringing smart microscopy to super-resolution MINFLUX: event-triggered MINFLUX microscopy.