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Posts by Pete Chuckran

The relationship between gene traits and transcription in soil microbial communities varies by environmental stimulus Codon and nucleotide frequencies are known to relate to the rate of gene transcription, yet how these traits shape transcriptional profiles of soil microbial communities remains unclear. Here we test ...

Our recent publication where the central question was: in soils, are there trademark characteristics of rapidly transcribed genes? And it was a resounding “it depends”. We found gene function, environmental stimulus, and phylogeny all impacted this relationship. More here:
peerj.com/articles/206...

1 month ago 2 1 0 0
RNeasy PowerSoil Total RNA Kit | Soil RNA Isolation | QIAGEN RNeasy PowerSoil Total RNA Kit is designed for the isolation of high-quality total RNA from all soil types

I've used Qiagen kits in the past which allows you to co-isolate DNA on the same spin-column.

RNA kit: www.qiagen.com/us/products/...

DNA co-isolation kit:
www.qiagen.com/us/products/...

1 year ago 3 0 0 0

Big thanks to @jeffinerca.bsky.social, @sieravirus.bsky.social, and other coauthors!

1 year ago 3 0 0 0
PNAS Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Wetting dry soil rapidly stimulates microbial activity and has important consequences for biogeochemical cycling. In our paper out today, we show that part of that response can be explained by how bacterial genomes are written (i.e. codon usage, nucleotide freq., genome size)
doi.org/10.1073/pnas...

1 year ago 35 10 1 1