Do you know how far phototoxicity can hinder your experiments?🤔 @mariodelr.bsky.social and @gomez-mariscal.bsky.social have just published their work on #PhotoFiTT in @natcomms.nature.com where they explain how to quantitatively measure it in live-cell imaging.⚡🧫🔬
Read more at: shorturl.at/ly9Zn
🚨Amazing news for a cloudy Monday!🌩️😎 I'm extremely happy to share that our last adventure is finally out!🚀🎉Phototoxicity fitness time trial (#PhotoFiTT) - a quantitative framework for assessing phototoxicity in live-cell microscopy experiments 🔬❤️🔥⚡️ doi.org/10.1038/s414...
🎉 Last but not least, a huge thanks to @gomez-mariscal.bsky.social Leonor Morgado, Raquel Portela, @guijacquemet.bsky.social @microbematos.bsky.social @henriqueslab.bsky.social
We have been an amazing team, and you made this work possible! 🥳🚀
🛠️Why this matters: PhotoFiTT empowers labs to fine-tune imaging conditions, improve reproducibility, reduce cell stress, and strengthen biological conclusions. All code, data & tools are open on GitHub & BioArchive 🔓📦 #OpenScience 👉 github.com/HenriquesLab...
💡 Key insights we uncovered: Near-UV light delays mitosis at doses as low as 0.6 J/cm². Longer wavelengths need higher doses, and surprisingly, continuous low-intensity illumination (slow scan) can be more damaging than brief high-intensity bursts (fast scan). Data-driven phototoxicity thresholds! ✨
🚀 Enter PhotoFiTT: a standardise experimental protocol combined with AI-powered image analysis to track cell behaviour (mitosis, size, activity) in response to light exposure. What’s even better: label-free and fully quantitative! 🔍🤖
The challenge: live-cell fluorescence microscopy can unintentionally stress or harm cells, changing timing, size and activity. If left unchecked, this can skew biology and standard qualitative checks just don’t cut it ⚡️ #Microscopy #CellBiology
It’s FINALLY out! 🎉🚀 We have been working on PhotoFiTT for a long time, and it’s now published, introducing a quantitative framework to measure phototoxicity and optimise live-cell microscopy experiments. So excited to finally share the last version! 🔬✨ #PhotoFiTT doi.org/10.1038/s414...
Fantastic work! Elegant experimental evolution with big implications for how cell size can evolve without compromising fitness. Congrats to Ana and collaborators, definitely worth a read for anyone into cell cycle, size control, or evolution 🧬🔄
Our Researcher @mariodelr.bsky.social and Raquel Portela from @microbematos.bsky.social were invited by @healthnewspt.bsky.social to talk about their new collaboration project awarded by Early Career Research Award, 𝗜𝗻𝗳𝗲𝗰𝘁𝗼𝗙𝗶𝗧𝗧.
Read the full interview at: tinyurl.com/infectofitt
Workshop attendees grouped together. In the background there are trees and mountains. The Workshop title is Democratising Microscopy in Latin America: Imaging Across Scales and Regions, and it took place in Banos, Ecuador on 12-15 October 2025.
Thank you to the organisers Constadina Arvanitis, @mariodelr.bsky.social, Helena De la Torre, @marianadeniz.bsky.social, María Fernanda Latorre Barragán and Licyel Lenny Paulas Condori.
Find out more about this week's Workshop: biologists.com/workshops/gl...
#BiologistsWorkshops
🔬 Community Workshop #2: "Stretching the Limits: Exploring Biology with Expansion Microscopy"
Learn how to unlock nanoscale #imaging with everyday microscopes using Expansion #Microscopy — #ExM.
🧑🔬Led by experts from @itqbnova.bsky.social: Carolina Feliciano and @mariodelr.bsky.social.
#SPAOM2025
Baños welcomed us with sunny skies!
One of our Ecuadorian organisers @mariodelr.bsky.social has been a key driving force behind this Workshop.
#BiologistsWorkshops
Learn more about Mario’s career path: focalplane.biologists.com/2023/08/22/a...
Last two days to apply for our extended deadline. Funded positions available!
Últimos dos días para aplicar. Posiciones financiadas disponibles!
Join us!
🌟 Join the UNESCO webinar on Remote Access to Imaging Facilities!
Discover real-world user cases and how imaging scientists, core facilities, and networks are shaping research worldwide.
Don't miss this chance to connect with experts!
🔗 Register now: tinyurl.com/UNESCO-GBI-w...
@unesco.org
A group of four COS cells - one seems to pull the other up like we all should do
The Nikon AX-NSPARC demo is gearing up at the
@ncis-marseille.bsky.social! Starting for #FluorescenceFriday with a large field-of-view confocal #cellfie of COS cells labeled for actin ⚫ endoplasmic reticulum 🟡 mitochondria 🔴 and nucleus 🔵. Fluorescence microscopy is also nice in inverted colors 🌈🧪
Apply for a funded early-career researcher place at @biologists.bsky.social Workshop on Democratising Microscopy in Latin America.
The Workshop will be in Baños de Agua Santa, Ecuador from 12 – 15 October 2025
Application deadline: 14 February
focalplane.biologists.com/2025/02/11/a...
Last day to apply! Come join us with a lineup of fantastic speakers!!
It was about time 😊
