Pov you're a deer

Love that the political strategist take away is that pesky nonreligious people ask too many questions and cost more to persuade

Characterizing the Content and Structure of AAV Capsids by Size Exclusion Chromatography and Orbitrap-Based Charge Detection-Mass Spectrometry Adeno-associated virus (AAV) is currently the most widely used vector in gene therapy applications. However, a significant challenge in the manufacturing process of recombinant AAV (rAAV) is the presence of empty capsids, oligomers, aggregates, and partially filled capsids. These components do not provide any therapeutic benefit but add to the overall viral load, which could increase immunogenicity and reduce transduction efficiency. Here, we present a strategy that utilizes size exclusion chromatography (SEC) equipped with multiangle light scattering (MALS) and a diode-array detector (DAD), followed by orbitrap-based charge detection-mass spectrometry (CD-MS). The SEC step was used to separate AAV capsids (non-aggregates) from oligomers, aggregates, and low molecular weight contaminants. In the second step, we employed direct CD-MS infusion using capillary electrophoresis with a sheath liquid (MS) interface. This approach facilitated automated, reproducible, sensitive, and robust CD-MS determination of empty-filled capsids, capsid oligomers, and encapsidated genomes. Importantly, the empty-to-filled capsid ratio was inaccurate without the SEC step. Together, our analytical platform offers a reliable and comprehensive approach for assessing the rAAV purity and characterizing key quality attributes, including capsid aggregation, capsid oligomerization, and genome packaging.

Characterizing the Content and Structure of AAV Capsids by Size Exclusion Chromatography and Orbitrap-Based Charge Detection-Mass Spectrometry #JASMS pubs.acs.org/doi/10.1021/...

Characterizing the content and structure of AAV capsids by size exclusion chromatography and charge detection mass-spectrometry

Authors: Kanchan Pathak, Gustavo Perrotti, Stephen Rosa, Graham Robinett, Lance Kasper, James Xia, Carlos Escalante, ...
DOI: 10.26434/chemrxiv-2025-ddxpm

Did you know Leaded gas is still used in some applications. Specifically aviation gas (avgas), used in old internal combustion planes. So everytime a prop plane flies over you, it's raining lead

what's your favorite crystal?
Me: Hexagonal close packed

Nothing is more exciting than seeing the results of an orderly atomic packing structure! I love crystals

I always felt like the lolcow community was very sad. It just seems like mass bullying.

Not really, it was hard to stay in contact with friends and family. I did enjoy leaving the house and feeling untethered. Driving to new places with a map and written directions was fun.

Its hard, I gave up my smart phone for a flip. I lasted about 6 months.

LLMs do not have lived experience in any scientific field. I would rather read a review written by human authors.

I wish the transparent blue was a little lighter but I like the look