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Posts by Krishna Mudumbi

8 people sitting in a conference room watching The Princess Bride on a projector screen

8 people sitting in a conference room watching The Princess Bride on a projector screen

Pizza, popcorn, and The Princess Bride.

Some Tuesdays just need to be a lab movie night!

1 week ago 1 0 0 0

Couldn't think of a better candidate to be on the ASCB Council!

1 week ago 4 1 1 0
A mocha frappuchino with a drawing of a cell on purple

A mocha frappuchino with a drawing of a cell on purple

I chat with the barista's at Starbucks about our research and they've taken to drawing cell related things on my coffee (one even visited the lab)! I present to you all a cilliated cell on my Frap!

Who says science communication can't be fun or tasty?!

3 weeks ago 6 0 0 0

Some of my favorite Vandy people highlighted here!

3 weeks ago 1 0 0 0

Is PI life modifying a grant proposal as you're finaling last minute things for your brother's wedding in T-minus 16 hours?!

Just have to make sure I don't bring up signal transduction in my speech or childhood stupidity in my grant!

1 month ago 1 0 0 0

Even then, it makes more sense to have it spread out over a few years so we can spend it on personnel, which makes more sense.

Also, I'm not hiring 8 people at once, even if I could

1 month ago 1 0 0 0

I would much rather have early-career researchers like myself have a higher chance to actually get a grant than get a grant that's frontloaded. I likely cannot spend it in a year anyway, unless we can now just buy expensive capital equipment like microscopes and mass specs on them, which I doubt.

1 month ago 6 2 1 0

The FOMO

1 month ago 1 0 0 0
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The Starbird lab has been doing such a great job of sharing excitement for our ongoing work with our Biophysics community!! #BPS2026

1 month ago 40 7 1 1

Ok, so this is pretty awesome actually!

1 month ago 1 0 0 0
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How X-rays and crystals revealed the true nature of things - NobelPrize.org The story behind the 100-year-old discovery that continues to render Nobel Prizes.

Very excited to share my love of crystallography in this article celebrating over 100 years of X-ray methods and the Nobel Prize for BBC and the Nobel Foundation:

www.nobelprize.org/stories/x-ra...

2 months ago 48 10 0 1

One Western is worth 7 meetings! Hopefully you got lunch

2 months ago 1 0 0 0

Thanks Tig, definitely looking forward to seeing how this line of research develops further!

2 months ago 1 0 0 0

Fellow new PI success PSA! @krishnamudumbi.bsky.social et al have an awesome preprint out! Super psyched to connect with @jasonzxzhang.bsky.social as well, I see collaborations in our future 😀

2 months ago 5 1 2 0

It was such a fun journey working with Krishna’s lab and @kinasekid.bsky.social! Really excited to see where this phospho-binder technology goes!

2 months ago 7 2 0 0
A graduate student in a pink hoodie and blue jeans pinning up her first publication on a cork board.

A graduate student in a pink hoodie and blue jeans pinning up her first publication on a cork board.

The inaugural pinning of a paper on our publications board!

Proud #NewPI moment!

2 months ago 1 0 0 0

Thanks Chrystal, so proud of my team!

2 months ago 1 0 1 0
De novo design of phosphotyrosine peptide binders Phosphorylation on tyrosine is a key step in many signaling pathways. Despite recent progress in de novo design of protein binders, there are no current methods for designing binders that recognize phosphorylated proteins and peptides; this is a challenging problem as phosphate groups are highly charged, and phosphorylation often occurs within unstructured regions. Here we introduce RoseTTAFold Diffusion 2 for Molecular Interfaces (RFD2-MI), a deep generative framework for the design of binders for protein, ligand, and covalently modified protein targets. We demonstrate the power and versatility of this method by designing binders for four critical phosphotyrosine sites on three clinically relevant targets: Cluster of Differentiation 3 (CD3ε), Epidermal Growth Factor Receptor (EGFR), Insulin Receptor (INSR) and Signal Transducer and Activator of Transcription 5 (STAT5). Experimental characterization shows that the designs bind their phosphotyrosine containing targets with affinities comparable to native binding sites and have negligible binding to non-phosphorylated targets or phosphopeptides with different sequences. X-ray crystal structures of generated binders to CD3ε and EGFR are very close to the design models, demonstrating the accuracy of the design approach. A designed binder to an EGFR intracellular region phosphorylated upon EGF activation co-localizes with the receptor following EGF stimulation in single-particle tracking (SPT) experiments, demonstrating pY specific recognition in living cells. RFD2-MI provides a generalizable all-atom diffusion framework for probing and modulating phosphorylation-dependent signaling, and more generally, for developing research tools and targeted therapeutics against post-translationally modified proteins. ### Competing Interest Statement The authors have declared no competing interest. NIH NCI, 1K99CA293001

Not new, but a new to us update:

The first preprint out of my lab! We joined forces with @kinasekid.bsky.social @jasonzxzhang.bsky.social and David Baker to study protein phosphorylation! Congrats to Isabella from my lab on her first first author paper! tinyurl.com/43jwwfua

2 months ago 23 4 2 3
Three people sitting around the board game Carcassonne with cookies and coffee

Three people sitting around the board game Carcassonne with cookies and coffee

Impromptu board games and cookies for those of us that lost power doing Snowmageddon!

2 months ago 2 0 0 0
8 people sitting at a long table with drinks and menus

8 people sitting at a long table with drinks and menus

Mini golf with @phdgprotein86.bsky.social and Jeeyeon Cha's labs, followed by pizza!

Great way to kick off the first lab outing of the year!

2 months ago 2 1 0 0
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A collage of two pictures. On the left is a picture of 6 people in a tissue culture room doing work. On the right is a picture of 4 people stacking two incubators on top of each other.

A collage of two pictures. On the left is a picture of 6 people in a tissue culture room doing work. On the right is a picture of 4 people stacking two incubators on top of each other.

At the one year mark, and we went from stacking incubators to a packed TC room!

#NewPI

3 months ago 8 0 0 0
A 35 year old letter to the editor, written by a very ballsy 15 year old, about the Rodney King verdict.

A 35 year old letter to the editor, written by a very ballsy 15 year old, about the Rodney King verdict.

Remembering today that having your heart broken is a necessary step on the path to becoming fully human. Whichever heartbreak is your first, it’s probably critical that a state break your heart so that you can develop a political imagination. If this is your first, I’m sorry and also welcome.

3 months ago 12158 2488 85 116
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Burnette Lab paper: "Pharmacological Inhibition of β Myosin II Disrupts Sarcomere Assembly in Human iPSC-Derived Cardiac Myocytes" is out! This image is of beta myosin II filaments in an iPSC-derived cardiac myocyte. #CellBiology
onlinelibrary.wiley.com/doi/10.1002/...

3 months ago 10 3 3 0

Anything from Tool really, but Ænima is a classic from the mid 90s

3 months ago 4 0 0 0
8 people sitting around a poker table with cards and poker chips on the table.

8 people sitting around a poker table with cards and poker chips on the table.

High stakes poker game at the CDB holiday party with my office at stake!

Rest assured that I managed to keep my office - the PI poker face is a real thing!

4 months ago 3 0 0 0
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What an amazing #CellBio2025! What I took away was that the cell bio community was just as vibrant as ever. Despite the challenges of the past year, I heard so much exciting science and celebrated many accomplishments of my friends and colleagues! Thank you! 🙏🏾

4 months ago 18 3 1 0

Happy Nobel Prize Day to those that celebrate! Thanks for highlighting our work its connection to Stanley Cohen!

4 months ago 8 1 0 0

Happening tomorrow! Bring your own coffee 😁

4 months ago 4 1 0 0
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Excited to host this session on Wednesday with these lovely ladies and some new friends that we made as well! Come check out the amazing talks that were selected!

4 months ago 6 2 0 0
5 people in front of the ASCB Cell Bio 25 sign

5 people in front of the ASCB Cell Bio 25 sign

Just two new PIs and our labs! @adelitadmendoza.bsky.social @ascbiology.bsky.social #CellBio25

4 months ago 24 3 0 0