Graphical abstract for pre-print showing a) theoretical model for thermostability, b) a GUI based analysis platform, c) protein denaturation data of mini-binders assessed by nanoDSF and d) a correlation plot that shows the de novo designed mini-binders have a much lower deltaCp than expected for a protein of their size.

Why are de novo designed proteins so extremely thermostable?

We try to answer this question by dissecting the thermodynamics of unfolding of mini-binders emerging from our protein design pipeline – and establish a convenient GUI for data analysis in the process.

doi.org/10.64898/202...

@cssbhamburg.bsky.social

New preprint out 🚀

De novo designed proteins are often too stable — but why?

Using CheMelt, we show that their exceptional thermostability is largely driven by unusually low heat capacity changes (ΔCp).

👉 www.biorxiv.org/content/10.6...

@embl.org @osvalb.bsky.social @proteinmagnus.bsky.social

The analysis tool is part of the eSPC package developed by @osvalb.bsky.social & @spcembl.bsky.social from EMBL Hamburg.

User-friendly analysis software for many biophysical techniques. Typically the eSPC software is better than that provided with the instrument:

spc.embl-hamburg.de

@cssbhamburg.bsky.social @lucasdefelipe.bsky.social

New on bioRxiv 🧪 📄

A fresh look at how domain-specific ligand binding controls TRPM2 activation ⛓️‍💥

This work disentangles the roles of distinct nucleotide-binding domains and their contribution to gating — a long-standing question in the field. 

👉 www.biorxiv.org/content/10.6...

@embl.org

Very exciting demo on smFRET &FCCS measurements in our SPC facility this week with Exciting Instruments @ei-science.bsky.social ✨🧮

Thank you for allowing us to measure single molecule resolution using your technology.

It has been again, very exciting 🔍🤩

@embl.org @cssbhamburg.bsky.social

@osvalb.bsky.social @cssbhamburg.bsky.social @embl.org

New protocol out now! 📕🖊️🔍

A practical workflow for analyzing CD thermal unfolding of short RNAs — from SVD decomposition to two-state fitting to extract melting temp and enthalpy with ChiraKit.

A must-see if you’re working with RNA structure and CD data.

DOI: 10.1007/978-1-0716-5084-4_12 

Clathrin adaptors drive phase separation in endocytosis and trafficking Liquid-liquid phase separation (LLPS) underlies the formation of biomolecular condensates that organize cellular processes, including endocytosis and membrane trafficking. Although LLPS has been impli...

New preprint out!📃

This work uncovers how the clathrin adaptors Ent5 & Sla2
undergo LLPS, revealing distinct structural drivers that shape endocytic and trafficking dynamics.

@lucasdefelipe.bsky.social @katharinchen.bsky.social @embl.org @cssbhamburg.bsky.social

👉 www.biorxiv.org/content/10.1...

@mpimarinemicrobio.bsky.social @cssbhamburg.bsky.social

New paper from our postdoc @fedevignale.bsky.social, et.al!

Studying microbialites in Argentina 🇦🇷, they show how extreme, early Earth-like conditions shape microbes, minerals & biosignatures- insights for Earth’s past and life beyond 🌎 🧬.

👉 www.nature.com/articles/s43...

@embl.org @ebi.embl.org

We’re looking for a GL!

Join us at EMBL Hamburg! 🧫🦠🧬

The position will be based at CSSB @cssbhamburg.bsky.social , an interdisciplinary research centre focused on infection biology, and will be integrated into EMBL's research programme. @embl.org

Find out more and apply here: lnkd.in/dJU9pee7

At EMBL Grenoble, lead an independent research group using bold, original approaches to integrate complementary structural biology methods with emerging nano X-ray imaging technologies, bridging scales and modalities to unravel molecular mechanisms.

embl.wd103.myworkdayjobs.com/en-US/EMBL/d...

Check it out the latest open position to join EMBL in Heidelberg!
@embl.org

Congrats to the Grünewald, Topf groups & et. al @cssbhamburg.bsky.social on their Nature paper! 📃 🍾

Trapping and resolving the elusive prefusion form of HSV gB - neutralizing HSV-1 & HSV-2 with a single nanobody is no small feat!🦙

Glad that our SPC facility have contributed to this work!@embl.org

Excited to be part of the Instruct Centre Germany!

@jankosinski.bsky.social @landaulabcssb.bsky.social @topf-lab.bsky.social @carobarisch.bsky.social @bosse-lab.bsky.social @spcembl.bsky.social

Exciting to be with part of my group at the 15th European Biophysics Congress (EBSA) in beautiful Rome, Italy 🇮🇹

If you are around, join my talk today about tools for the analysis of biophysical data 💡🧩

Check our posters for open discussion and networking !📃🖇️

@embl.org
@cssbhamburg.bsky.social

KinGenie, an online tool for the analysis of kinetics data, has been introduced on the 4th MOSBRI Scientific Conference in Paris, France 🇫🇷

A great addition to our eSPC web server💡🚀

Reach out to us for further input!

@osvalb.bsky.social
@mosbri.bsky.social
@embl.org
@cssbhamburg.bsky.social

4th MOSBRI Scientific Conference starts today 📍in beautiful Paris, France 🇫🇷

Excited to see familiar and new faces from our TNA site, network and continue collaborating on research infrastructures projects in molecular biophysics 🧬🧫 🤝

@mosbri.bsky.social @embl.org @cssbhamburg.bsky.social

Happy to co-host a workshop on lipid nanoparticles together with @embl.org Hamburg this week. The workshop is a part of the @eugloh.bsky.social ‘Entrepreneurship meets Health Innovation at Large-Scale Facilities’ conference.

@landaulabcssb.bsky.social @spcembl.bsky.social

Our latest research is out 📄 !

This study provides an interaction map of Sla2 & the regulatory proteins Sla1 & Pan1, predicted by AI modeling & confirmed by molecular biophysics.

@lucasdefelipe.bsky.social @meytallandau.bsky.social
@emilgustavsson.bsky.social
@embl.org
@cssbhamburg.bsky.social

ChiraKit: an online tool for the analysis of circular dichroism spectroscopy data Abstract. Circular dichroism (CD) spectroscopy is an established biophysical technique to study chiral molecules. CD allows investigating conformational ch

Now publication NAR is online:

doi.org/10.1093/nar/...

This project was conceived as a collaboration in the frame of MOSBRI @mosbri.bsky.social

🔊 Thrilled to share our latest work, just published in NAR! 📃

We present ChiraKit, open-source tool to process raw CD data and perform advanced analysis!

Access via our eSPC web-server!

➡️ spc.embl-hamburg.de
@osvalb.bsky.social @lucasdefelipe.bsky.social @embl.org @cssbhamburg.bsky.social

Tanks for the nice feedback! Peer-reviewed publication coming soon 😉👍

ChiraKit, an online tool for the analysis of circular dichroism spectroscopy data Circular dichroism (CD) spectroscopy is an established biophysical technique to study chiral molecules. CD allows investigating conformational changes under varying experimental conditions and has bee...

Just want to say thanks to @spcembl.bsky.social for developing ChiraKit and allowing quick and informative analysis of CD data - more here www.biorxiv.org/content/10.1...
best news of the day today as we were stuck with beautiful but hard to analyze data 🙂 #scienceisawesome

We're hosting an Ask Me Anything about Biophysics on Bluesky today with BPS Ambassador Alumnus Gonzalo de Prat Gay! Tweet your questions with #BiophysicsAMA and tune in at 11am USA Eastern time.

🚨The registration for the Functional #Amyloid Meeting is finally open, check it out on landau-cssb.de

🧪🧬🧶

Congratulations to George who had his PhD defense today! 🥂🍾🥳🎉 @uni-hamburg.de @embl.org @cssbhamburg.bsky.social