Color merged microscopy image: magenta is bulk protein, green bulk DNA, and orange nuclear pore complex (NPC).

Bulk DNA staining, displayed as single color channel.

Nuclear pore complex, displayed as single color channel.

Bulk protein, displayed as single color channel.

For #FluorescenceFriday, I am sharing a first glimpse at Mona Wellhäusser's @mofrawe.bsky.social new nuclei expansion microscopy.

Stained are bulk protein, bulk DNA, and nuclear pore complexes (NPC). Expansion is supposed to be 16x, in all three dimensions. Imaged with @visitech.bsky.social iSIM.

Most of the current Hilbert lab members and Master student interns in the weekly lab meeting.

Almost all hands on deck today in Hilbert lab meeting. Postdoc Ewa very small on Zoom, PhD student Nils substituted for a colleague for a talk; associated fellow Simon and Master student Georg not in the photo. I just felt super happy I can work with this group, so I am sharing selfie greetings 👋 😃

First hours of embryonic zebrafish development. Credit to Mona Wellhäusser, Timo Schreiber, & @lennarthilbert.bsky.social. #ZebrafishZunday 🧪

Great post to start the new year, the first hours of a new life :-)

Also tagging @mofrawe.bsky.social here, her handle is a tad difficult to find, but worth to watch and follow ;-)

Last night Science Slam with a full house at Tollhaus, as part of @kit.edu Science Week. As one of four slammers, our PhD student Mona Wellhäusser @mofrawe.bsky.social entertained a huge audience, putting fish in jello for microscopy, (optically) slicing a pumpkin. Great show, fantastic atmosphere!

If you are at GBM compact "Focus on Condensate Biology", we will be happy to talk to you at our lab's posters:

Mona Wellhäuser, MC15 @mofrawe.bsky.social
Yuzhi Bao, CA03 @yuzhibao.bsky.social
Lennart Hilbert, MC06

Conference abstracts:

gbm-compact.org/abstracts.html

*sketch added as click bait

Um das Verhalten der DNS nachvollziehbar und vorhersagbar zu machen, kombinieren die Forschenden Laborexperimente mit Computersimulationen.

Molekulare #Bionik: Der Zellkern als Vorbild für DNS-basierte #Computerchips. Forschende des #KITKarlsruhe (u. a. @lennarthilbert.bsky.social) nutzen zelluläre Informationsverarbeitung als Inspiration für die #Biotechnologie. www.kit.edu/kit/pi_2025_...

An analogy between the architectures of electronic computers and transcription factories. (A) Von Neumann computer architecture, in which a CPU is connected to different blocks in a working memory. (B) A transcription factory centrally stores the molecular transcription machinery and can selectively access genes. (C) The operation principle of a CPU-memory architecture based on synthetic DNA nanostructures.

"Chromatin-associated condensates as an inspiration for the system architecture of future DNA computers"

Highly conceptual Perspective article, supported by fresh data & simulations from our group. Containing my now 40 years of accumulated "wisdom".

nyaspubs.onlinelibrary.wiley.com/doi/10.1111/...

Our first expansion microscopy results. Imaged today on "normal" Zeiss LSM 900, revealing chromatin and RNA polymerase II clusters at an unreal level of detail.

Our PhD student @mofrawe.bsky.social learned the protocol from amazing @mpownall.bsky.social 🙏

Months of preparation are paying off now!

Posters & pizza workshop event between @kreysinglab.bsky.social , Levkin lab, and ourselves Hilbert lab, also joined by Miha Modic @paraspeckle.bsky.social

Now already the second annual repeat, third will make it a tradition 😀

It also happened to be Pavel’s bday, hence cake and champagne 🥂

Genetik: Wie springende DNA das Erbgut umkrempelt | Lennart Hilbert KIT Pressemitteilung zu unserer kürzlich veröffentlichten Studie im Biophysical Journal. Mit Roshan Prizak und Aaron Gadzekpo als Erstautoren, super Arbeit! https://lnkd.in/esdBphJ5

Guten Morgen an unsere deutschsprachigen Lesenden 🌞

Hier die @kit.edu Pressemitteilung zu unserer kürzlich erschienenen Arbeit im Biophysical Journal.

Wir untersuchen, wie bewegliche Gensequenzen das Genom “umkrempeln“

www.linkedin.com/posts/lennar...

#FluorescenceFriday fixed cell data over the cell cycle in zebrafish embryo, Hoechst & IG against active enhancers and recruited Pol II. Recorded by @mofrawe.bsky.social on @visitech.bsky.social iSIM, prepared during Masters practical, credit also to @yuzhibao.bsky.social & TA Alicia Günthel

One-cell-stage to 21-somite-stage development of a zebrafish embryo The video shows the development of a zebrafish embryo from one-cell-stage to 21-somite-stage. Unedited videos, as well as a sped-up version are available. Videos were recorded and edited by Mona Wellhäusser and Timo Schreiber in the research group headed by Lennart Hilbert at the Institute of Biological and Chemical Systems, Karlsruhe Institute of Technology. Full data and citable version on Zenodo: https://doi.org/10.5281/zenodo.12547356

A developing @zebrafishrock.bsky.social egg for the weekend ☺️

youtu.be/8Jm-CrbtXYY?...

Recorded and prepared by PhD student @mofrawe & Bachelor student Timo Schreiber.

For full data and description: doi.org/10.5281/zeno...

📌