Very intriguing finding made possible by recent advances in mono-ADPr detection

The paper describing our sensitive poly-ADPr antibodies and a few other things is now out in Nature Communications. Congrats to all the authors!!
www.nature.com/articles/s41...

ADP-ribosylation is far more diverse than the usual mono vs poly view.
New sensitive tools from the Ivan Matic lab to resolve this complexity are a big step forward — expanding what more labs can now study.

www.nature.com/articles/s41...

At low dilution (~0.1–1 µg/mL), AbD64138 SpyTag HRP enables detection of otherwise elusive poly-ADPr. At high dilution (~0.01–0.1 µg/mL), it can reduce costs ~10× compared to other tools when poly-ADPr levels are abundant.

While also available in synthetic rabbit/mouse IgG and biotinylated formats, the community will particularly benefit from the SpyTag HRP format. As with the widely used mono-ADPr antibody AbD43647 (Longarini et al. Mol Cell 2023), the high sensitivity of SpyTag HRP has two important advantages.⬇️

Obtained by extending our programmable Ser-ADPr technology (Bonfiglio et al. Cell 2020) through a new phage display strategy, this first modular poly-ADPr antibody detects species from di-ADPr to long chains with no cross-reactivity toward mono-ADPr (preprint below; manuscript under revision).

We’ve made the poly-ADPr antibody AbD64138 available in a SpyTag HRP-conjugated format (TZA0117P):
www.bio-rad-antibodies.com/monoclonal/h...

This sophisticated format enables detection of very low poly-ADPr levels while simplifying and reducing the cost of detecting abundant poly-ADPr.

🎉 Celebrating our Amazing Women in Ageing Research! 🎉
Meet Xinping, scientific staff in the proteomics core facility 👩‍🔬
#WomenInScience #AgeingResearch

Our third PhD student, and third summa cum laude. Congrats, Andreas!

🎉 Celebrating our Amazing Women in Ageing Research! 🎉
Meet Maria, Postdoc in the Matic Lab 👩‍🔬 Maria studies how our cells respond to damage in their DNA — the instruction manual of life. 🧬
@mariapalumbieri.bsky.social

We were delighted to have Nobel laureate Venki Ramakrishnan with us to discuss his exciting new book, "Why We Die." Thanks so much, Venki, for your fascinating and thought-provoking talk - it was truly a pleasure to have you join us!

Apply now for a fully funded #PhD position at the Cologne Graduate School of Ageing Research and become part of our vibrant #AgingResearch commmuny.
🗓️ Deadline: November 3, 2025.
ℹ️ More info: www.ageing-grad-school.de
📣 Spread the news!

ADPr gets a ubiquitin upgrade - Nature Chemical Biology MARUbylation is a hybrid post-translational protein modification in which mono-ADP-ribosylation acts as a scaffold for sequential mono- and polyubiquitylation. Recent studies illuminate its substrates...

Superb News&Views on our recent paper reporting identification of ADP-ribosyl-linked serine ubiquitylation
www.nature.com/articles/s41...

• Together with a series of papers and preprints published this year, it establishes RNF114 as a reader of ADPrUb

• Observation that serine mono-ADPrUb increases in ARH3 KO cells, raising the intriguing question of its potential role in ARH3 mutation–associated neurodegeneration

• First reagent specific for ADPrUb, enabling both immunoblotting and immunoprecipitation

• First mass spectrometric evidence of ADPrUb, regardless of the target residue

• Serine mono-ADPrUb as a new type of DNA damage-induced histone modification

• First evidence that, in cells, serine mono-ADPr by the PARP1/HPF1 writer complex is targeted by this unconventional ubiquitylation

At its core, it’s a technological paper—necessary to overcome some of the many challenges of working with ADPrUb. But beyond the technical details, below are the main highlights:

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. A proteomics approach is developed using these domains to map ADP-ribosyl-ubiquitylation sites

www.nature.com/articles/s41...

Thrilled to share my first first-author postdoc publication — a team effort with @aklvnbch.bsky.social. We present the first MS-based evidence of ADP-ribosylation–linked ubiquitin (ADPrUb) in cells after DNA damage.
Grateful for all I’ve learned and looking forward to uncovering more about ADPrUb

Really happy that our paper is finally released. We show that PARP1 and histones are targets of ADP-ribosyl-linked ubiquitylation during DNA damage and describe how to detect the dual modification. Great teamwork with @mariapalumbieri.bsky.social & thanks to everyone involved @maticlab.bsky.social !

Serine ADPr on histones and PARP1 is a cellular target of ester-linked ubiquitylation - Nature Chemical Biology RNF114 is an E3 ligase that can recognize ADP-ribose (ADPr) and ubiquitin with separate domains. Using these domains, Kolvenbach and Palumbieri et al. developed a proteomics approach to map ADP-ribosy...

Our latest study on ADP-ribosyl-linked serine ubiquitylation in the context of PARP1 signaling and the DNA damage response is out today in Nature Chemical Biology www.nature.com/articles/s41...

Edoardo Longarini receives the Peter Hans Hofschneider Prize Edoardo Longarini receives the prestigious Peter Hans Hofschneider Prize for his exceptional work in molecular medicine.

Huge Congratulations to Edoardo José Longarini on receiving the prestigious Peter Hans Hofschneider Prize! 🎉

www.age.mpg.de/424030/Edoar...

Discover the CGA film Get to know our people & programme

🎥We're excited to share the new film about our graduate programme. Get a glimpse of our faculty & PhD students and explore your research opportunities. Join us as we dive into the world of ageing research and discover what makes the CGA truly unique.👩‍🔬🧑‍🎓 www.ageing-grad-school.de/about-us/cal...

dELTA-MS: A Mass Spectrometry-Based Proteomics Approach for Identifying ADP-Ribosylation Sites and Forms ADP-ribosylation, characterized by the addition of adenosine diphosphate ribose, can occur in both monomeric (MARylation) and polymeric (PARylation) forms. Little is known about the specific contribut...

New Paper💡PARP enzymes regulate ADP-ribosylation, a protein modification linked to cancer, infections & neurodegeneration. But it comes with two forms—MARylation (single) & PARylation (multiple). Meet dELTA-MS, a mass spec method that identifies both in one experiment!
pubs.acs.org/doi/full/10....

Ubiquitin is directly linked via an ester to protein-conjugated mono-ADP-ribose | The EMBO Journal imageimageCertain E3 ligases have been found to ubiquitylate hydroxyl groups on free NAD+ and ADP-ribose in vitro, but the in vivo occurrence of this dual post-translational modification has remained ...

I'm super excited to share our study in collaboration with @jnpruneda.bsky.social, published today in @embojournal.org! We show that PARPs are modified with a non-canonical ester-linked mono-ADP-ribosylated ubiquitin species for the first time in cells: www.embopress.org/doi/full/10....

These new antibody development efforts align with our vision that the fundamental discovery of Serine ADP-ribosylation can be converted into a technology that transforms the study of ADP-ribosylation and related PTMs.

This preprint is part of a larger manuscript on additional antibodies and their applications to biological questions. The goal of this preprint is to share these poly-ADPr with the community without delay, as we believe they wil benefit many laboratories.