View of Stockholm's Old Town (Gamla Stan) from Södermalm
Was great to be back in Stockholm for #FOM2026 together with others from the @lasergroup.bsky.social. Enjoyed presenting recent work on lighsheet/OPM for cleared and expanded samples and learning about the latest developments in microscopy! 🔬
🙋♀️
🔬Nearly 20 years ago, @imperialphysics.bsky.social's Professor Christopher Dunsby at @imperialcollegeldn.bsky.social invented a new microscopy technique. This week, @leicamicrosystems.bsky.social launched a commercial product built around it: www.imperial.ac.uk/news/article...
We hope this will open up further applications for fast 3D imaging of tissues and complex structures with sub-cellular resolution, using conventional sample mounting techniques.
Thank you to everyone who made this work possible.
Full details 👉 doi.org/10.1364/BOE.... (🧵 6/6)
We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and > 1 cm long mouse bone 🐭🦴 (🧵 5/6).
Using RI-tuned bead phantoms, we show that correction collar adjustment can partially compensate for RI mismatch, improving the imaging depth for a range of clearing protocols 🧪 (🧵 4/6).
We demonstrate imaging of higher refractive indices (RI), maintaining a sub-1.5 µm axial resolution over hundreds of microns in depth, with multi-view image fusion providing more isotropic resolution (🧵 3/6).
We present cleared-tissue optimized single-objective dual-view light-sheet fluorescence microscopy using:
🔹Folded remote-refocusing OPM
🔹1.25 NA silicone-immersion primary objective
(🧵2/6).
✨ Now out in Biomedical Optics Express: Cleared tissue dual-view oblique plane microscopy 🔬: doi.org/10.1364/BOE.... (🧵1/6)
We hope this will open up further applications for sub-cellular resolution 3D imaging of tissues and complex structures, using conventional sample preparation and mounting techniques 🔬.
Thank you to everyone who made this work possible.
Full details 👉 doi.org/10.1364/BOE.... (🧵6/6)
Animation of fluorescence microscopy data of a fly brain
We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and >1 cm-long mouse bone 🐭🦴 (🧵5/6).
Figure showing bead images for a range of sample refractive indices and correction collar positions
Using RI-tuned bead phantoms, we show that correction collar adjustment can partially compensate for RI mismatch, improving the imaging depth for a range of clearing protocols 🧪 (🧵4/6).
Two figures, one showing orthoslices through a bead volume, the second showing the X,Y,Z FWHM variation with imaging depth
We demonstrate imaging of higher refractive indices (RI), maintaining a sub-1.5 µm axial resolution over hundreds of microns in depth, with multi-view image fusion providing more isotropic resolution (🧵3/6).
Diagram of a dual-view OPM system showing the light path rays for the two views
We present cleared-tissue optimized single-objective dual-view light-sheet fluorescence microscopy with a folded remote-refocusing OPM system configured with a 1.25 NA silicone-immersion primary objective (🧵2/6).
A diverse group of people smiling, gathered in a modern lounge with red carpet and wooden walls, suggesting a casual, friendly event.
Last week, our Early Career Researchers (ECRs) and Postdocs Committee hosted their first-ever departmental social.
These are the events where ideas spark and collaborations take root.
🧪⚙️🌱🔋
#ChemicalEngineering #Biotechnology #CambridgeUniversity
@rogerrubiosanchez.bsky.social
🔬Our preprint list is now up on FocalPlane.
This week we focus on new tools and techniques in imaging. Let us know if we are missing any recent preprints that you've enjoyed reading.
focalplane.biologists.com/2025/06/13/m...
Hello Bluesky! 🦋
New preprint from my postdoctoral work back at @imperialcollegeldn.bsky.social - design, characterization and application of a dual-view oblique plane microscope 🔬(dOPM) for cleared tissue imaging over a range of refractive indices.
www.biorxiv.org/content/10.1...
