Thanks and congrats to lead authors Irfana Saleem and Thomas Miller and the other co-authors for their incredible work on this project.

We are very excited about the long-term potential of msDMS-MaP to improve understanding of RNA biology and enable applications such as RNA targeted drug discovery. The experiments are remarkably easy, so if you are using DMS, I encourage you try out msDMS-MaP yourself!

This additional data enables direct discovery and annotation of tertiary motifs and protein binding sites. In two examples in our paper, we use msDMS-MaP uncover new relationships between rRNA tertiary folding and ribosome assembly, and new protein binding sites on the human 7SK RNA in cells.

msDMS-MaP is a simple extension of standard DMS probing experiments that helps solve this limitation. We developed a new strategy that can simultaneously measure multiple types of DMS modifications that report on RNA secondary structure (N1/N3) AND higher-order structure (N7-G).

As many know, DMS probing experiments are amazing at mapping RNA secondary structure, but are less capable at identifying higher-order tertiary structures and protein binding sites. Because higher-order structures are often the most functionally interesting, this represents a significant limitation.

Multi-site DMS probing reveals higher-order structure of RNA-protein complexes in living cells Saleem et al. introduce multi-site DMS-MaP (msDMS-MaP), a chemical probing strategy that permits simultaneous mapping of RNA secondary structures and typically concealed tertiary structures and protei...

Pleased to share our latest paper, out today in Molecular Cell: “Multi-site DMS probing reveals higher-order structure of RNA-protein complexes in living cells”

www.cell.com/molecular-ce...

Check out the new method that reveals structures of #RNA-protein complexes in living cells. @amustoe.bsky.social @bcmhouston.bsky.social @cp-molcell.bsky.social www.eurekalert.org/news-release...