Evaluating the Volta phase plate for improved tomogram alignment in cryo-electron tomography We use sub-2 Å single-particle analysis and analytical contrast transfer function modeling to show that the Volta phase plate (VPP) boosts low-frequency signal relevant for cryo-electron tomography (c...

People interested in the VPP aspects should also see a recent paper (from the people at Biohub) showing that tilt-series alignments can be improved with phase plate usage.

journals.iucr.org/m/issues/202...

Figure 4 from the preprint

We @embl.org wrote a short preprint about optimizing for maximum completeness in #cryoet analysis. We looked at template matching, VPP imaging, and tilt-series refinement parameters, and why averaging ribosomes helps even if you aren’t interested in their biology 🏭

www.biorxiv.org/content/10.6...

Really excellent work by the bluesky-less Marie and Sara +coauthors in our lab @embl.org! Check it out for cool combinations of light microscopy and #cryoET, and if you're interested in how yeasts reorganize structurally in response to stressors. #teamtomo

Congratulations, Julia Mahamid!

Julia Mahamid receives the @dfg.de Leibniz Prize for her work on structural cell biology!

#LeibnizPreis

I was also going to point out it being a bit slow, but was waiting to see my job complete first! Thanks for figuring it out. I also noticed that the tutorial config.yaml may have the wrong names for fields e.g. "tomo1" needs to be "tomo1_files" for it to run

Thanks!

Thanks!

Thanks! According to their stats page (www.ebi.ac.uk/emdb/statist...) I should be at around 37% right now, but we'll have to see how the rest of the year goes! 📈

Thanks!

Thanks! I didn't see h44 repositioning despite directly looking for it. Could be the P-tRNA, but I also suspect IF3 might be involved in clamping it down in some way (>90% of 30S had IF3).

Thanks! Although my wrists might disagree :)

Sadly not :( I got most of the model IDs sequential, but the maps were a different story

In-cell discovery and characterization of a non-canonical bacterial protein translocation-folding complex Cryo-electron tomography has emerged as powerful technology for in-cell structural biology, and in combination with breakthroughs in protein structure prediction, offers a unique opportunity for illum...

Thanks! The dome is definitely intriguing, and happily I can point you to this nice recent preprint by my colleague @rasmusjensen.bsky.social about that exactly www.biorxiv.org/content/10.1...

Thanks!

Thanks!! Very true, and things are getting more and more doable- I think it'll be quite a different place in a few years.

Figure 1 from the preprint

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️

www.biorxiv.org/content/10.1...
#teamtomo

The small GTPase Ran defines nuclear pore complex asymmetry Nuclear pore complexes (NPCs) bridge across the nuclear envelope and mediate nucleocytoplasmic exchange. They consist of hundreds of nucleoporin build…

My PhD paper is in press @cellpress.bsky.social and now online: www.sciencedirect.com/science/arti...!

Contents: weird #NPCs in yeast and flies, #CLEM, in situ #cryoET and more!

Made possible by the amazing @becklab.bsky.social team at @mpibp.bsky.social and collaborators! #TeamTomo

New paper from my colleague Soheil in the Mahamid and @jonasries.bsky.social groups looking at factors influencing laser devitrification on frozen #cryoem samples. With a far-red fluorophore, all-gold support grids will be especially good for correlative cryo-SMLM and EM!

doi.org/10.1016/j.js...

Are you into cryo-ET, in-cell discovery, protein translocation and folding or just cool integration of structural biology, proteomics and bioinformatics? Then see our new work:

www.biorxiv.org/content/10.1...

Thank you to all coauthors for your amazing work!