That's good to know! Would be interesting to see how widespread adoption already is, and what the limits are.

It's a great story. Lowering a FAIMS electrode temperature compromises resolving power and greatly increases transmitted peptide ions, while still filtering out troublesome singly charged background.

www.nature.com/articles/s41...

Speculation, but TIMS devices empty as they scan out; perhaps populations with lower average mobility thus suffer less from global space charge.

For local effects, M+ ions occupy a smaller spatial distribution as mentioned.

Maybe dividing precursors into m/z or intensity bins will be revealing.

Good observation and not all that intuitive. Presume that saturation of both is still dominated by Tims process rather than ToF.

Could it simply be lower charge density of 1+ ions on average during elution (or emptier trap by the time they emerge on average)?

I just had one last dinner with John Syka, before he moves back to Virginia for retirement later this week. I am indescribably grateful for the opportunity to work with him over the last eight years at Thermo. #TeamMassSpec (1/N)

Poster for the Analytical Research Forum (ARF26) by the Royal Society of Chemistry. Event on 15 June 2026 in London, UK. Oral abstract extension deadline: 23 March 2026. "Submit now" button is visible. Background features a stylized abstract design.

There’s still time to share your analytical chemistry research at the Analytical research forum (ARF26).
Oral abstract deadline is now 23 March. 📆 Submit your abstract today: rsc.li/arf2026 #ChemSky

Hardest part is that she doesn't know what a scam these cards are.

Were there many reasons given not to fill one up like a foie gras goose?

Wouldn't say any of it is really dumb, and they're all questions one might ask oneself.

The next step for it might be how to discard an idea. For example, computational burden of real time analysis (and rescoring?), dynamic range on mega-multiplexing, electrodynamic signals on ToF mass range etc.

That may be too much to ask! The really advanced algorithms are a bit too severe for millisecond response times.

But maybe room for more general predictions...

Indeed the automated analysis is very detached from both the priorities and even (what I would consider) the biggest problems.

Get ready for the bombardment of crazy requests and ideas..!

Good news!

I like the thought that an LLM might come up with, and highlight, the sort of niche connections a serious expert might occasionally make in the shower.

Can it though. How does one ask?

Any interest in taking multiple scans per precursor at different mass ranges, or are losses more than acceptable? 😀

Works on multiple levels! 😄

Aye, it all derives from the 2022 application. There's some mechanism behind claims in these additional documents.

Number of ions imposes a limit, but things can always be worse.

For context, label free quan peptide-level CV is ~10% typically, if I recall correctly.

The mass range of tandem mass spectra can be categorized into three main regions. For glycopeptides, the lower mass range contains mainly oxonium ions, the middle range contains mainly peptide fragments, and the high range contains charge-reduced fragments. All regions contain valuable fragment ion information, but they can only be simultaneously accessed by breaking the 5–10–15 rule.

MS/MS scan range can be an afterthought that doesn't receive attention in method design, but improper settings can affect experimental outcomes. This is especially true in glycoproteomics.

A #glycotime thread about a new #JASMS paper on this idea from @riley-research.bsky.social

1/10

Alas no! We have a regime of industrial design guidance to make every product harmonised, user friendly, intuitive... and a white box with a black front and a blue pulsing light.

The box looks much better, but the lighting is a devastating loss.

Finally released the preprint covering TMT HR mode for the Orbitrap Astral Zoom mass spectrometer. Here, multi-pass MS/MS measurements grant us sufficient resolution to quantify TMT reporter ions even to 35-plex, greatly increasing analytical throughput.

www.biorxiv.org/content/10.6...

Sounds reasonable to me, and this need to reduce co-isolation pulls us to long gradients, good chromatography etc.

Idle speculation: Perhaps in Astral data, as many PSMs are filtered out from quantification (but there are a lot of PSMs), strong interferences may be preferentially filtered.

I would expect 15 ions in Astral gives better reproducibility, if only because it's much further above the noise line. I think it shouldn't be hard to dig up CVs.

But indeed, on the higher load (non degraded HeLa sample), it averages >100 ions per peak.

In defense of MS2 TMT, use of FAIMS and advances in quadrupoles allow ~90% interference free index, similar to MS3 prior to real time search no?

I'd show RTS MS3 if we'd kept the original plan and built the Orbitrap Astral MS with an ion trap, but we'd never have finished the project.

Timing here is that this study combines data from multiple groups, a lot of it presented last ASMS, but the task of aggregating results and writing up wasn't owned by anyone. Took me a little while to confirm.

Curious! I've seen comparisons to mass spec (albeit not in agreement, while MS vs MS is ok), but it should be no problem to assess via controlled samples.

I know people for whom such investigation, with mass spectrometers, is much of their jobs.

Are there not standard-based quantitative, multi-proteome or matrix matched serial dilution experiments?

The sort of thing that's very standard in assessing mass spectrometers?