And here it is! Our study in clonal hematopoiesis and Alzheimer’s disease — product of 5 years’ gestation. urldefense.proofpoint.com/v2/url?u=htt...

A lot of fun teaming up with Hsin and Howard on this, with key contributions from @ruochiz.bsky.social, @zchiang.bsky.social, and @jbuenrostro.bsky.social on the tech dev and @mattjones.bsky.social, Natasha Weiser, and Paul Mischel on the ecDNA analysis. (5/5)

We also apply SPACE-seq to an archived glioblastoma sample, showing that SPACE-seq works well with clinical samples and can detect extrachromosomal DNA amplifications and somatic mutations. (4/5)

Mitochondrial DNA is captured as well, enabling mtscATAC (a lineage tracing technique previously developed by @caleblareau.bsky.social, @leifludwig.bsky.social, and @bloodgenes.bsky.social) to be applied spatially. (3/5)

The main idea here is that by tagmenting tissue sections with polyA-tailed adapters, instead of the standard ATAC-seq adapters, we can use spatial transcriptomics slides from @10xgenomics.bsky.social to capture both ATAC and RNA data with spatial resolution. (2/5)

Happy to share our paper describing SPACE-seq - a new approach to spatial multiomics and lineage tracing - out today in @pnas.org! 🧵(1/5)

Today we're thrilled to announce our 2024 #HannaGrayFellows! Please join us in welcoming and celebrating these outstanding early career scientists!