SPRINGER NATURE: Open Science | Step-by-step guides. Share your research code and protocols openly. Best practices for transparency and reuse. Read the blogs. Abstract blue and purple design element on right.

Strengthening transparency and reproducibility starts with sharing research work openly.

Our latest blogs offer guidance for:
> How to share research code openly:
spklr.io/63329Eynzz
> How to share research protocols and methods openly:
spklr.io/63322EynzO

Attending ABRF 2026 (@abrf.bsky.social)? Connect with protocols.io at Booth 710. We're engaging with core facilities about common priorities and challenges, including how to demonstrate impact and receive credit. If this resonates, schedule a short discussion with our team: abrf26.youcanbook.me

Three-dimensional genome reorganization foreshadows zygotic genome activation in Drosophila - Nature Genetics Pico-C, a low-input Micro-C approach, reveals that dynamic three-dimensional genome folding precedes zygotic genome activation in Drosophila.

Excited to share my PhD work!

The question: How is genome organization established? Here, we developed a low-input Micro-C method, Pico-C, and used it to map chromatin architecture across early stages of fly development.

Here’s a quick Blue-torial 🙂 (1/n)

www.nature.com/articles/s41...

Webinar poster: “Enhancing Impact in Core Facilities,” April 9, 11 AM EST / 5 PM CET.

Join our webinar to hear from researchers at the University of Rochester and City of Hope on practical strategies for improving documentation, supporting training, and increasing visibility of core facility work.

April 9 | 11 AM EST | 5 PM CET
Register here: go.springernature.com/enhancing-im...

Protocol.io logo

Last month, we highlighted Protocols.io as a new resource available at @bodleian.ox.ac.uk. Next week, as part OxFOS, you can sign up and join the Springer Nature team for a training session introducing the platform to researchers either in person here at the RSL or online

go.glam.ox.ac.uk/proto

Started this project years ago, but now everything is deposited on @addgene.bsky.social and a short @protocolsio.bsky.social is online. Try it out to make your own (cheap) homemade nuclease/Benzonase!

dx.doi.org/10.17504/protocols.io.261ged7xov47/v2

Thank you @jcoker10.bsky.social and Michael Lim!

We've updated our protocols.io page to highlight a couple of the protocols published by Crick authors recently.

If you're at the Crick, don't forget you can get your flat pdfs converted to the protocols.io format at no cost! 😀

www.protocols.io/organization...

Labeling of nascent RNA in the C. elegans intestine Transcriptional regulation in C. elegans has been difficult to study at the level of nascent RNA because nucleotide analogs do not readily penetrate the cuticle. Here, we establish an ex vivo…

A Lab Protocol by Omid Gholamalamdari and Stephanie C. Weber details an optimized method for labeling of nascent RNA transcripts in the C. elegans intestine, enabling spatial analysis of transcription dynamics.

plos.io/3Ox4rnO

@protocolsio.bsky.social link: plos.io/3O9Jv6g

Generation of Stickleback intestinal organoid Intestinal organoids offer significant advantages over conventional 2D cultures, providing a physiologically relevant 3D architecture that enables more realistic disease modelin...

New @protocolsio.bsky.social post describing our new protocol for generating intestinal organoids for #stickleback: www.protocols.io/view/generat...
With work by postdoc Arshad Padhiar, funded by @moorefound.bsky.social

Given the recent #Nipah outbreak in India, here are two #Nanopore protocols for NiV genomic sequencing in support of rapid outbreak response:

IPC: www.protocols.io/view/sequenc...
icddr,b/CDC: journals.asm.org/doi/10.1128/...

Promotional graphic for Springer Nature and protocols.io featuring an event announcement. The left side has a light blue background with the Springer Nature logo at the top and text reading: “Join experts for interactive sessions. Jan 21: protocols.io Hackathon. Jan 22: Methods Mythbusting Panel.” A dark blue button below says “Register today.” The right side shows the protocols.io logo and a large text design that says “I ♥ Methods” with a red heart symbol, followed by “Love Methods Week” and the hashtag #LoveMethods26.

💡 Did you know? Methods are among the most cited research outputs.
For #LoveMethods26, join experts for interactive sessions:
👉 Jan 21: @protocolsio.bsky.social Hackathon
👉 Jan 22: Methods Mythbusting Panel
spklr.io/633288eAVM

#OpenScience

We will import and format your protocol Paper protocols floating on your benchtops? We can digitize them for you!

Happy International Love Methods week!

The Crick partners with @protocolsio.bsky.social to share open methods, and if you happen to be a Crick researcher, you can use their protocol writing up service for free (while codes last!)

www.protocols.io/we-enter-pro...

Join protocols.io and Springer Nature editors at Love Methods Week 2026 Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...

Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26!

💻Transform your protocols at our Hackathon on Jan 21

🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22

👉FREE registration & more details:

Join protocols.io and Springer Nature editors at Love Methods Week 2026 Love Methods Week 2026 will take place January 19-23, 2026 and is organized by University of Coimbra and the QUEST center. Online workshops focused on open and reproducible meth...

Join Springer Nature editors and the protocols.io team for two exciting events as part of #LoveMethods26!

💻Transform your protocols at our Hackathon on Jan 21

🔮Gain insight on methods publishing at our Methods Mythbusting on Jan 22

👉FREE registration & more details:

Graphic representing our monthly New Resource promotion featuring the name of the Library and this months chosen product - Protocols.io

Our promoted new resource for January is Protocols.io. Using Protocols.io researchers can create and share detailed experimental protocols with colleagues or the wider scientific community

www.protocols.io

Join us for “Methods Mythbusting: Insights from Journal Editors and protocols.io”protocols.io/webinars/met...rs

at Love Methods Week 2026excelscior.uc.pt/love-methods...6/

#LoveMethods26

🎉 We are thrilled to announce that our protocol “Library construction for ONT long read cDNA”, developed at our GenomiqueENS core facility, is available on protocol.io. dx.doi.org/10.17504/protocols.io.4r3l21oepg1y/v1

🧬Searching for a Nanopore RNA-seq protocol that generates longer reads than the manufacturer’s protocol, and applicable for low-input material ? 🤔

Really liking the idea and output of GAQETv2 - such a cool tool for structural genome annotation QC!!
www.protocols.io/view/structu...

Exciting news: Premium protocols.io is now available via Research4Life to researchers in low- & middle-income countries—at no or low cost.

🌟 Full Springer Nature press release: group.springernature.com/gp/group/med...

#OpenScience #Reproducibility #GlobalCollaboration #Research4Life #protocolsio

The image promotes a new collaboration empowering researchers to share methods, improve reproducibility, and drive global scientific progress. It includes a close-up of a computer chip with a brain illustration and the Springer Nature logo.

Springer Nature has extended its partnership with @r4lpartnership.bsky.social to provide researchers in more than 90 low-middle-income countries with no-cost or low-fee access to premium features on @protocolsio.bsky.social.

Learn more: group.springernature.com/gp/group/med...

#Research 🧪

The Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub) ...

Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub librehub.github.io) is an amazing initiative from @makertobey.bsky.social and colleagues!

We have done our first @protocolsio.bsky.social. Are you interested about #genome_annotation quality evaluation? We have developed a tool called #GAQET2 (github.com/victorgcb198...) which runs several tools like #AGAT, #BUSCO, and many other tools. You can find it at www.protocols.io/view/structu...

Construyendo protocolos científicos reproducibles y abiertos con Protocols.io YouTube video by LIBRE hub

It was great to hear from @lteytelman.bsky.social about Protocols.io today for our LIBRE hub channel on open science! We already have a tutorial in Spanish: youtu.be/RuBKWouEyL8?...

With the full CRISPR 7-11 protocol here :)

Graphic with a dark teal background featuring three illustrated circular icons representing different research teams. On the left, Team Kordower icon shows colorful antibody shapes labeled “Neuro-Immune Interactions.” In the center, Team Liddle icon depicts a stylized brain with interconnected circuitry labeled “Circuitry and Brain-Body Interactions.” On the right, Team Rio icon shows a human head outline with a DNA strand inside, labeled “PD Functional Genomics.” A thin gradient line runs along the bottom, shifting from purple to blue to green, with the ASAP logo in the lower right corner.

👏 This month’s Network Spotlight goes to Teams Kordower, Liddle, & Rio!

Their open-access protocols ranked among the top 100 most viewed on @protocolsio.bsky.social in August 🎉

➡️ Mouse Perfusions (bit.ly/4qH6rIY)
➡️ Y-Maze (bit.ly/47aITTC)
➡️ Culture/transfection of HEK293T cells (bit.ly/4o4ytfR)

Optimisation of whole cell human depletion provides increased sensitivity and microbial genome coverage from respiratory metagenomic assays Metagenomics is being adopted worldwide as a laboratory developed test. We have previously reported a unified metagenomic method for the direct detection of microorganisms from respiratory samples, de...

Our new preprint covering the improvements we made to our respiratory metagenomic assay is out now:

www.medrxiv.org/content/10.1...

✅ Now implemented in 6 NHS hospitals
✅ Increased sensitivity
✅ Increased pathogen genome coverage

(1/3)

‘REF needs dedicated section for research methods’ - Research Professional News Hidden REF Festival: Conference hears plea to fill gap in Research Excellence Framework

Here’s a write-up that includes my lightning talk and reflections on why technicians must be recognised in the REF:
🔗 www.researchprofessionalnews.com/rr-news-uk-r...
Hope to work with others to get methods formally recognised as reportable research outputs in a category of their own.

The Spatial Thinking Ability Test (STAT-24):Classroom administration, scoring, and data management This protocol standardizes the classroom administration, scoring, and data management of the Spatial Thinking Ability Test (STAT-24), a category-balanced instrument for secondar...

3. The Spatial Thinking Ability Test (STAT-24):Classroom administration, scoring, and data management
www.protocols.io/view/the-spa...

Water and sediment sampling in the sea to discover marine bacteria This protocol aims to show the strategy for obtaining water and sediment samples on board an oceanographic vessel at sea. The samples obtained can be used to identify marine bac...

2. Water and sediment sampling in the sea to discover marine bacteria
www.protocols.io/view/water-a...