Replication program of a single-chromosome budding yeast strain Abstract. Nuclear architecture and chromosome folding are often speculated to influence genome replication. In yeasts, centromeres cluster close to the spi

New paper! Loss of the Rabl configuration in single-chromosome yeast yields only local replication changes, measured by nanopore sequencing with NanoForkSpeed and Nanotiming. academic.oup.com/nar/article/...

G-quadruplex–stalled eukaryotic replisome structure reveals helical inchworm DNA translocation DNA G-quadruplexes (G4s) are non–B-form DNA secondary structures that threaten genome stability by impeding DNA replication. To elucidate how G4s induce replication fork arrest, we characterized fork ...

I am so proud to share a major part of my graduate school research, published today in Science, where we use cell-free replication assays and cryo-EM to describe a central mechanism of eukaryotic DNA replication:

www.science.org/doi/10.1126/...

Telomere-to-telomere DNA replication timing profiling using single-molecule sequencing with Nanotiming - Nature Communications Current temporal studies of DNA replication are either complex or low-resolution. Here, the authors introduce Nanotiming, a simple and inexpensive method producing high-resolution, telomere-to-telomer...

NanoTiming is now in Nature Communications! High-resolution DNA replication timing using nanopore sequencing shows that Rif1 regulates only specific telomeres: www.nature.com/articles/s41...