What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~
www.biorxiv.org/content/10.6...
Out in @natmethods.nature.com: More dyes. They work. Quite well. And blink. Pick the one that fits your target, your technique, and your labeling density. With too many collaborators and institutes to list, but anchored at @hhmijanelia.bsky.social www.nature.com/articles/s41...
How do proteins find their way to the cell's leading edge? @jimgalbraith27.bsky.social & @cathygalbraith.bsky.social break down how bleach line, FLOP, single-particle tracking, FCS & iPALM reveal intracellular fluid flow and an actin-myosin compartment barrier. doi.org/10.1038/s414...
is there a direct crosstalk between actin and vimentin intermediate filaments? Our work shows that vimentin promotes actin assembly by stabilizing ATP-subunits at the barbed end.
Fantastic work done by @lilianpaty.bsky.social with @romet-jegou-lab.bsky.social
www.biorxiv.org/content/10.1...
Presenting a talk the ASCB @ascbiology.bsky.social #CellBio2025 meant a lot to me, a new guy in the field. Thanks to everyone whom came for the session, and special thanks to the ones whom dropped me kind encouragements before my talk! 😊
Today, our animation synthesizing decades of research on actin-mediated endocytosis in budding yeast was published:
journals.biologists.com/jcs/article/...
The result of a fantastic Iwasa-Drubin lab collaboration.
@margotriggi.bsky.social @jiwasa.bsky.social
movie.biologists.com/video/10.124...
Hey everyone and fellow #actin people! Here's a preview video for my upcoming talk at #cellbio2025 @ascbiology.bsky.social next Wednesday at 10am! Because it's gonna happen on the last day, so I promise to be a little funny, and I might even throw in a song and dance! And some nice data too.
Time projection images of mast cells (RBL-2H3) expressing active mutant of formin mDia3, captured by TIRFM.
The softcopy version of my submission!
Realized this morning that my submission got selected for Yale's Beauty in Science 2025 exhibition! Seeing the live-assembly of filopodia projections by formin mDia3 in mast cells got me really stoked back then! #FluorescenceFriday
🧵5/6 — The Big Picture
EndoNB works on endogenous proteins. No rare antibodies. No overexpression.
It’s scalable and ready to explore the 1000+ surface proteins we’ve barely studied.
We’re done understanding vesicular trafficking by looking at a handful of model surface proteins.
🧵1/6 — Preprint Alert!
I am super proud to present the latest work of our lab. Earthlings and those just visiting, I introduce you…. EndoNB: A better, smarter and versatile way to study the dynamics of cell surface proteins. 🧪
www.biorxiv.org/content/10.1...
My first post on Bluesky! Very excited to share our work just published in @science.org. We find that “Interphase cell morphology defines the mode, symmetry, and outcome of mitosis” - in angiogenesis and other tissues! www.science.org/doi/abs/10.1... www.science.org/doi/abs/10.1...
We show that under low ahesion conditions Tensin 1 forms endogenous condensates to store inactive adhesion components. These can be released upon stress-mediated Tensin 1 phosphorylation to regulate adhesion dynamics.
Happy to share our Tensin 1 phase separation project is now on bioRxiv.
www.biorxiv.org/content/10.1...
This wouldn't be possible without
@johannaivaska.bsky.social
@ivaskalab.bsky.social
#VattulainenLab
#MSCA by @ec.europa.eu
@turkubioscience.bsky.social
@inflames-flagship.bsky.social
New preprint alert!📣 @midibu.bsky.social and team take a deep dive into Tensin1 liquid-liquid phase separation (LLPS) and the mechanisms of how it regulates adhesion dynamics!
Check out the preprint here: www.biorxiv.org/content/10.1...
@johannaivaska.bsky.social
Unlike eggs & mitotic cells, in adherent interphasic cells the centrosome is NOT positioned by a balance of forces along #microtubules, but by cell shape organization around the #centrosome: rupress.org/jcb/article/...
@lblanchoin.bsky.social
@manuelthery.bsky.social
@gelinmatthieu.bsky.social
Progress on the quest for a red mStayGold!
Just out: mScarlet3-S2, a new photostable red FP derived from mScarlet3: www.researchsquare.com/article/rs-5...
Incredible 4Pi and pan-expansion imaging of the Golgi 👇
Thanks for selecting my entry. This is one of my favorite movies captured. I’m very flattered 🙂
Hi everyone, because it's my first time winning any contest, I ain't gonna lose this wave of opportunity to talk about it 👀. I got tweeted, but I feel the need to publicize this here too. I won the @ascbiology.bsky.social ASCB 2024 Image Contest! Thanks for selecting my entry!
Jerry with his prop to showcase waves
Jerry laughing at all the good questions and grilling
It was a humbling experience being able to talk to people about our works at the ASCB. I’m flattered that I had plenty audience for me to keep going without break for ~6 h straight and going overtime! Thanks, everyone 🥹 #CellBio2024 @ascbiology.bsky.social
#FluorescenceFriday An old video of a cute mast cell (RBL-2H3) exhibiting cortical waves of active Cdc42 after getting treated with cytochalasin-D overnight. Sorry not sorry to kill #actin hehe. #TIRF 4 s per frame acquisition, 8 fps, 32× real time.
I’ve been using cloudconvert.com/avi-to-mp4 to convert video formats heh. It allows you to adjust the resolution, codecs etc, if this is something you are looking for?
I will be representing @wumin-lab.bsky.social group to present our recent work at ASCB, on Monday Dec 16th, 11:15 AM! Board Number B19, P1714. Come have a chat! I'd probably also be dropping teasers on where our follow-up study is heading 😎
Look at these distinct clusters exhibited by N-WASP and Arp2/3 ! Is branched #actin polymerization the sole driving force for nucleating cortical actin waves? Cross-talks within the actin network can be messy, so how can we study such complexities? #CellBio2024 @ascbiology.bsky.social
Mix two motors of opposite polarity with microtubules and they will partition space !
Microtubules will get organized in polar and active barriers, sorting the two motors in separated domains, leading to the emergence of a new type of patterns.
#morphogenesis
doi.org/10.1073/pnas...
(1/n)
Before I start posting about newer results on this platform, here is a video from our most recent paper -- www.nature.com/articles/s41...
If you're searching for the Mechanobiology community and starter pack, here it is:
Thought this would be a great opportunity to gather the #mechanobiology community. Please feel free to spread the word.
go.bsky.app/G5cQqYT
here's the starter pack for cytoskeleton
go.bsky.app/TF83cpi
A still from a cine film down a microscope showing dark patches in two cells that are recognisable to a cell biologist nowadays as focal adhesions. The view is through the eyepieces i.e. circular, as though we are spying on the cells.
Who needs immunofluorescence?
Focal adhesions (or cell feet as they were known then) were first observed in this 1975 paper by Abercombie and Dunn
doi.org/10.1016/0014...
This gem comes from the obituary of Graham Dunn published in J Cell Sci today.
doi.org/10.1242/jcs.... 🧪
