AboutScience (@aboutscience) Bsky

AboutScience <p><strong>About. Open. Science.</strong><br />Open science is what we are about. We publish open access journals fostering science in clinical and experimental medicine, HTA, digital health, patient outcomes and everything else that matters in healthcare.</p>

@aboutscience.bsky.social is an #OpenAccess multidisciplinary publisher focused on #ClinicalMedicine and #Healthcare in its broadest term, including #DigitalInnovation, health technology assessment, #NarrativeMedicine, and other topics of growing impact: www.scienceopen.com/collection/b...

1 year ago 1 1 0 0

Characterization and enhanced antibiofilm activity of <i>Annona muricata</i> extract in combination with fluconazole against <i>Candida albicans</i> <div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="st1">  </a> <h5 class="section-title" id="d8978410e212">Introduction:</h5> <p dir="auto" id="d8978410e214"> <i>Candida albicans</i> biofilm formation is a significant contributor to antifungal resistance, necessitating new treatment strategies. <i>Annona muricata</i> Lin., a traditional herbal remedy, has shown promise in combating microbial infections. The purpose of this study was to assess the antibiofilm activity of the methanol extract of <i>A. muricata</i> leaves alone or with the addition of fluconazole against <i>C. albicans</i>. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="st2">  </a> <h5 class="section-title" id="d8978410e228">Methods:</h5> <p dir="auto" id="d8978410e230">Phytochemicals from the methanol extract were analyzed by LC-MS, the XTT assay was used for metabolic activity, and morphological characteristics were examined using scanning electron microscopy (SEM). Molecular docking screening of identified compounds in <i>A. muricata</i> methanol leaves extract against a Sap3 receptor (PDB: 2H6T) was also performed. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="st3">  </a> <h5 class="section-title" id="d8978410e236">Results:</h5> <p dir="auto" id="d8978410e238">The LC-MS analysis detected 17 possible phytochemicals. The methanol extract showed a dose-dependent inhibition of biofilm formation, with maximum inhibition of ~60% observed at 240 μg/ml, and inhibition by fluconazole increased from 32% to 76% as the concentration increased from 15 to 240 μg/ml. The combination of <i>A. muricata</i> and fluconazole increased the inhibition significantly, from 74% to 78% at 15 μg/ml to 240 μg/mL, respectively. SEM of control and treated <i>C. albicans</i> biofilms showed an altered morphology and loss of cell integrity by the combination, corroborating the findings. Plant phytochemicals also possess high binding affinity (−9.7 to 8.0 kcal/mol, respectively) for the Sap3 enzyme and may therefore have therapeutic potential against <i>C. albicans</i>. </p> </div><div xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="section"> <a class="named-anchor" id="st4">  </a> <h5 class="section-title" id="d8978410e250">Conclusion:</h5> <p dir="auto" id="d8978410e252">Consequently, the findings indicate that compounds in the <i>A. muricata</i> methanol extract may function in concert with fluconazole at sub-inhibitory concentrations to suppress <i>C. albicans</i> biofilm formation. This finding paves the way for the formulation and development of antifungal treatment regimens that may limit the development of fluconazole resistance employing this plant part. </p> </div>

'Characterization and enhanced antibiofilm activity of Annona muricata extract in combination with fluconazole against Candida albicans' - an @aboutscience.bsky.social article on #ScienceOpen: www.scienceopen.com/document?vid...

1 year ago 0 1 0 0

This study examined rock climbing’s effects on #Parkinson’sdisease. After 12 weeks, 28 participants improved mobility, agility, dexterity, and reaction time, suggesting climbing benefits functional movement | journals.aboutscience.eu/index.php/ao...

1 year ago 1 0 0 0

The XXII National Conference on Pharmaceuticals focused on medicine assessment, new regulations, collaboration, #orphandrug evaluation, and AIFA’s reorganization to optimize pricing and reimbursement. | journals.aboutscience.eu/index.php/gr...

1 year ago 1 0 0 0

📣Call for Section Editors: Drug Repurposing

Drug Target Insights is launching a #CallForEditor for a new section dedicated to #DrugRepurposing.

📌 Full details are available here journals.aboutscience.eu/index.php/dt...

1 year ago 1 0 0 0

This study explores #bioethicalissues in #cancerrehab, highlighting dilemmas in disclosure, balancing hope, treatment safety, and withdrawal decisions, stressing the need for a patient-centered approach.

journals.aboutscience.eu/index.php/ao...

1 year ago 3 0 0 0

This study assessed #RSV impact in #Italy and the benefits of #nirsevimab. Current strategies protect only high-risk infants, but nirsevimab could cut cases, complications, and costs in their first season.

journals.aboutscience.eu/index.php/gr...

1 year ago 2 1 0 0

This study used MCDA for Orphan Drugs to compare #leniolisib vs. standard of care in APDS. A panel found leniolisib superior in efficacy, safety, QoL, and cost, making it a valuable treatment.
journals.aboutscience.eu/index.php/gr...

1 year ago 2 0 0 0

This study evaluated #Annonamuricata leaf extract alone and with fluconazole against #Candidaalbicans. It inhibited biofilms (60%), enhanced with fluconazole (78%). Docking suggested Sap3 binding for antifungal potential.

journals.aboutscience.eu/index.php/dt...

1 year ago 2 0 0 0

Posts by AboutScience