5. Many thanks to @stoplab.bsky.social, @dpulimamidi.bsky.social , Martyna Plens-Galaska, Maxime Lalonde, @mgkopczynska.bsky.social, Shazia Irshad, Hiroshi Kimura, @michal-gdula.bsky.social, and Stephan Hamperl for a great collaboration on this project. 6/6

4. Shortening of the distance between pre-mRNA cleavage and RNAPII terminal pausing is associated with higher gene expression❗️
Shortened spacing = higher expression BUT also higher addiction to PCF11 and CPSF73. 5/6

3. Comparing metastatic with primary tumor CRC cells, we again observed that changes in PAS selection and RNAPII terminal pausing are not unidirectional (proximal PAS & distal RNAPII terminal pausing). 4/6

2. In our model, primary tumor cells display a tendency for selection of distal polyadenylation site (PAS), and at the same time, proximal (!) shift of RNAPII terminal pausing as compared to normal colon cells (= #uncoupling & shortened spacing between PAS and termination). 3/6

1. Colorectal cancer (CRC) cell clonogenicity critically depends on #PCF11 and #CPSF73, with primary tumor cells showing much higher addiction to those factors than metastatic cells. Why??? 🤔2/6

Did you know that changes in pre-mRNA 3' cleavage and RNAPII termination can go in different directions? Even more, the spacing between these two events is linked to the gene expression level❗️Check out our latest pre-print to learn more tiny.cc/330w001 1/6