The third EM structure of a Bfu type bifurcating complex coming out of the Adams - Li collaboration. Wor-Bfu is a cool W containing complex that can detoxify aldehydes and found in a number of gut organisms. It is our third article in PNAS on W in the gut microbiome🧪
www.pnas.org/doi/10.1073/...

After a long time of trying, finally a structure of the NADP(H) dependent NiFe hydrogenase from Pyrococcus furious. 🧪
kwnsfk27.r.eu-west-1.awstrack.me/L0/https:%2F...

Characterization of a W storage protein. Illustrating the importance of W in the gut 🧪
Shao N, Storage of the vital metal tungsten in a dominant SCFA-producing human gut microbe Eubacterium limosum and implications for other gut microbes. mBio0:e02605-24.https://doi.org/10.1128/mbio.02605-24

The resolution of blue sucks. It is a good second column if your protein cooperates.

Blue can be the best purification ever or you never see your protein again. You can do a salt gradient to 2 M followed by a nad(p) block elution.

Cryo-EM Structures Define the Electron Bifurcating Flavobicluster and Ferredoxin Binding Site in an Archaeal Nfn-Bfu Transhydrogenase Flavin-based electron bifurcation couples exergonic and endergonic redox reactions in one enzyme complex to circumvent thermodynamic barriers and minimize free energy loss. Two unrelated enzymes desig...

#2 out of 3 bfu family bifurcating structure with our CryoEM collaborators.
Cryo-EM Structures Define the Electron Bifurcating Flavobicluster and Ferredoxin Binding Site in an Archaeal Nfn-Bfu Transhydrogenase - Journal of Biological Chemistry www.jbc.org/article/S002...

Cryo-EM reveals a composite flavobicluster electron bifurcation site in the Bfu family member NfnABC - Communications Biology Structural analysis of a thermophilic bacterial NfnABC complex reveals for the first time that the minimum electron bifurcating site of the Bfu family is a flavobicluster consisting of FMN, a [4Fe-4S]...

The first of a series of 3 cryoEM structures from our collaborative efforts is shedding some light in the Bfu type electron bifurcating complexes. The Bfu type bifurcating complexes utilize a combination of flavin and iron sulfur clusters to bifurcate electron pairs.
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A simple Tungsten and molybdenum assay for biological samples. Excellent work by an UGA undergraduate student. 🧪
bio-protocol.org/en/bpdetail?...

Thx Jennifer

Tungsten is utilized for lactate consumption and SCFA production by a dominant human gut microbe Eubacterium limosum | PNAS Eubacterium limosum is a dominant member of the human gut microbiome and produces short-chain fatty acids (SCFAs). These promote immune system func...

Tungsten enzymes and lactate metabolism in the human gut microbiome. 🧪
www.pnas.org/doi/10.1073/...

Tungsten enzymes play a role in detoxifying food and antimicrobial aldehydes in the human gut microbiome | PNAS Tungsten (W) is a metal that is generally thought to be seldom used in biology. We show here that a W-containing oxidoreductase (WOR) family is div...

Tungsten as a trace element for the human microbiome 🧪
www.pnas.org/doi/full/10....

I would like to join the science feed.
Thx Gerrit
Gerrit Jan Schut (orcid.org/0000-0002-36...) 🧪

Tungsten is utilized for lactate consumption and SCFA production by a dominant human gut microbe Eubacterium limosum | PNAS Eubacterium limosum is a dominant member of the human gut microbiome and produces short-chain fatty acids (SCFAs). These promote immune system func...

www.pnas.org/doi/10.1073/...

Pissed off Christmas cats 😄

That is awesome congrats